ID A0A3A9J693_9PROT Unreviewed; 378 AA.
AC A0A3A9J693;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Putative glutamate--cysteine ligase 2 {ECO:0000256|HAMAP-Rule:MF_01609};
DE EC=6.3.2.2 {ECO:0000256|HAMAP-Rule:MF_01609};
DE AltName: Full=Gamma-glutamylcysteine synthetase 2 {ECO:0000256|HAMAP-Rule:MF_01609};
DE Short=GCS 2 {ECO:0000256|HAMAP-Rule:MF_01609};
DE Short=Gamma-GCS 2 {ECO:0000256|HAMAP-Rule:MF_01609};
GN ORFNames=D6Z83_18135 {ECO:0000313|EMBL:RKK02747.1};
OS Pseudoroseomonas wenyumeiae.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Pseudoroseomonas.
OX NCBI_TaxID=2478470 {ECO:0000313|EMBL:RKK02747.1, ECO:0000313|Proteomes:UP000278036};
RN [1] {ECO:0000313|EMBL:RKK02747.1, ECO:0000313|Proteomes:UP000278036}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Z24 {ECO:0000313|EMBL:RKK02747.1,
RC ECO:0000313|Proteomes:UP000278036};
RA Tian Z.;
RT "Roseomonas sp. nov., isolated from feces of Tibetan antelopes in the
RT Qinghai-Tibet plateau, China.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: ATP-dependent carboxylate-amine ligase which exhibits weak
CC glutamate--cysteine ligase activity. {ECO:0000256|HAMAP-Rule:MF_01609}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01609};
CC -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 2 family.
CC YbdK subfamily. {ECO:0000256|HAMAP-Rule:MF_01609}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKK02747.1}.
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DR EMBL; RAQU01000129; RKK02747.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3A9J693; -.
DR InParanoid; A0A3A9J693; -.
DR OrthoDB; 9769628at2; -.
DR Proteomes; UP000278036; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0042398; P:cellular modified amino acid biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.590.20; -; 1.
DR HAMAP; MF_01609; Glu_cys_ligase_2; 1.
DR InterPro; IPR006336; GCS2.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR011793; YbdK.
DR NCBIfam; TIGR02050; gshA_cyan_rel; 1.
DR PANTHER; PTHR36510; GLUTAMATE--CYSTEINE LIGASE 2-RELATED; 1.
DR PANTHER; PTHR36510:SF1; GLUTAMATE--CYSTEINE LIGASE 2-RELATED; 1.
DR Pfam; PF04107; GCS2; 1.
DR SUPFAM; SSF55931; Glutamine synthetase/guanido kinase; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01609};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01609, ECO:0000313|EMBL:RKK02747.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01609};
KW Reference proteome {ECO:0000313|Proteomes:UP000278036}.
SQ SEQUENCE 378 AA; 41519 MW; 64E2A80D285150A1 CRC64;
MTASYRFGIE EEYFLADATT RGTPGPTVAG FHAAVRDRLP EAERELLQSQ VEVASPPSTD
FAEARGVLSG LRRGLADIGR EHGVLVLAAG THPLAQWSSQ DHTEAERYEG LMRDLRMLGR
RNVVCGMHVH VEVPRPEARI DLMNRLMPFM PLLLGLSASS PFWQGQETGL AAYRLSVFGE
MPRTGLPDLF HDAEDYARYV EIMTRAGAIK DASFLWWTLR PSIKFPTLEL RVADACTRLE
DTIAIAALYR CLVRCADRRP ELNRGMTGTS RALVAENLWR AQRDGARAML IDEAKGEAVP
FADALDTLLA LVAEDAEALG CAPDISALRR IASEGTSADR QATVFARARQ GGASEREALG
AVVDWLARTT RGEEKVGE
//
