ID A0A3A9JEA5_9PROT Unreviewed; 402 AA.
AC A0A3A9JEA5;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=3-oxoadipyl-CoA thiolase {ECO:0000256|ARBA:ARBA00012233};
DE EC=2.3.1.174 {ECO:0000256|ARBA:ARBA00012233};
GN Name=pcaF {ECO:0000313|EMBL:RKK04882.1};
GN ORFNames=D6Z83_07110 {ECO:0000313|EMBL:RKK04882.1};
OS Pseudoroseomonas wenyumeiae.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Pseudoroseomonas.
OX NCBI_TaxID=2478470 {ECO:0000313|EMBL:RKK04882.1, ECO:0000313|Proteomes:UP000278036};
RN [1] {ECO:0000313|EMBL:RKK04882.1, ECO:0000313|Proteomes:UP000278036}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Z24 {ECO:0000313|EMBL:RKK04882.1,
RC ECO:0000313|Proteomes:UP000278036};
RA Tian Z.;
RT "Roseomonas sp. nov., isolated from feces of Tibetan antelopes in the
RT Qinghai-Tibet plateau, China.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + succinyl-CoA = 3-oxoadipyl-CoA + CoA;
CC Xref=Rhea:RHEA:19481, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:57292, ChEBI:CHEBI:57348; EC=2.3.1.174;
CC Evidence={ECO:0000256|ARBA:ARBA00000708};
CC -!- PATHWAY: Aromatic compound metabolism. {ECO:0000256|ARBA:ARBA00005211}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000256|ARBA:ARBA00010982, ECO:0000256|RuleBase:RU003557}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKK04882.1}.
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DR EMBL; RAQU01000029; RKK04882.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3A9JEA5; -.
DR InParanoid; A0A3A9JEA5; -.
DR OrthoDB; 9764638at2; -.
DR Proteomes; UP000278036; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0033812; F:3-oxoadipyl-CoA thiolase activity; IEA:UniProtKB-EC.
DR GO; GO:0019619; P:3,4-dihydroxybenzoate catabolic process; IEA:InterPro.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012793; PcaF.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020610; Thiolase_AS.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR NCBIfam; TIGR02430; pcaF; 1.
DR PANTHER; PTHR43853; 3-KETOACYL-COA THIOLASE, PEROXISOMAL; 1.
DR PANTHER; PTHR43853:SF2; 3-OXOADIPYL-COA_3-OXO-5,6-DEHYDROSUBERYL-COA THIOLASE; 1.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00737; THIOLASE_2; 1.
DR PROSITE; PS00099; THIOLASE_3; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003557,
KW ECO:0000313|EMBL:RKK04882.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000278036};
KW Transferase {ECO:0000256|RuleBase:RU003557, ECO:0000313|EMBL:RKK04882.1}.
FT DOMAIN 5..269
FT /note="Thiolase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00108"
FT DOMAIN 278..401
FT /note="Thiolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02803"
FT ACT_SITE 90
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 358
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 388
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
SQ SEQUENCE 402 AA; 42324 MW; 2D27551D6E821161 CRC64;
MTEAFICDAI RTPIGRYAGS LAPVRADDLG AVPIKALMAR NPGLDWSALD DVIFGCANQA
GEDNRNVARM SSLLAGLPDT VAGSTINRLC GSGMDATGTA ARAIKSGEAE IMLAGGVESM
TRAPFVQGKS TEAFSRAAEI YDTTIGWRFV NPLMKAQYGV DSMPETGENV AQDFQISRQD
QDLFAFRSQQ RAKAAMESGR FDEEIVPVEI KRRKGDPIIF SKDEHPRPET TLEALAKLGT
PFRKEGGTVT AGNASGVNDG AAAILVASEA AAGRYGLTPR ARVVAMATAG VPPRIMGFGP
APATKKVLEK AGLTLADIDV IELNEAFASQ ALAVMRDLGL PDDAEHVNPN GGAIALGHPL
GMSGARIVMT AVAELHRRKA RYALATMCIG VGQGIAMVLE RV
//
