ID A0A3A9JX26_9PROT Unreviewed; 387 AA.
AC A0A3A9JX26;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE SubName: Full=Mandelate racemase {ECO:0000313|EMBL:RKK03609.1};
GN ORFNames=D6Z83_13760 {ECO:0000313|EMBL:RKK03609.1};
OS Pseudoroseomonas wenyumeiae.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Pseudoroseomonas.
OX NCBI_TaxID=2478470 {ECO:0000313|EMBL:RKK03609.1, ECO:0000313|Proteomes:UP000278036};
RN [1] {ECO:0000313|EMBL:RKK03609.1, ECO:0000313|Proteomes:UP000278036}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Z24 {ECO:0000313|EMBL:RKK03609.1,
RC ECO:0000313|Proteomes:UP000278036};
RA Tian Z.;
RT "Roseomonas sp. nov., isolated from feces of Tibetan antelopes in the
RT Qinghai-Tibet plateau, China.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR634611-3};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR634611-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKK03609.1}.
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DR EMBL; RAQU01000078; RKK03609.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3A9JX26; -.
DR InParanoid; A0A3A9JX26; -.
DR OrthoDB; 9802699at2; -.
DR Proteomes; UP000278036; Unassembled WGS sequence.
DR GO; GO:0047808; F:D(-)-tartrate dehydratase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd03326; MR_like_1; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR034611; D-tartrate_dehydratase.
DR InterPro; IPR034593; DgoD-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR PANTHER; PTHR48080:SF5; D(-)-TARTRATE DEHYDRATASE; 1.
DR PANTHER; PTHR48080; D-GALACTONATE DEHYDRATASE-RELATED; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR SFLD; SFLDF00118; D-tartrate_dehydratase; 1.
DR SFLD; SFLDS00001; Enolase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 4: Predicted;
KW Magnesium {ECO:0000256|PIRSR:PIRSR634611-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR634611-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000278036}.
FT DOMAIN 161..258
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
FT ACT_SITE 182
FT /note="acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT ACT_SITE 320
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT BINDING 19
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 53
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 100
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 154
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 180
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 211
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 237
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 237
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 263
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 263
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 320
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT SITE 53
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 180
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 290
FT /note="Increases basicity of active site His"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 339
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
SQ SEQUENCE 387 AA; 42946 MW; C822D0F6622D5059 CRC64;
MRIVDVCEIT QPIASPIRNA YIDFTKMTTS LVAVVTDVVR DGRRVVGYGF NSNGRYGQGG
LIRERFRDRI LEAAPESLRD DARDNLDPGK VWAAMMRNEK PGGHGERSVA VGTLDMAVWD
AVAKIEGKPL FRLLAERHGR EADPRVFVYA AGGYYYPGKE NGALRAEMRG YLDRGYNVVK
MKIGGASVAE DRARIEAVLA EIGSEARLAV DANGRFDLET AIAHAKMLRD YPLFWYEEAG
DPLDYALQAA LAEFYPNPMA TGENLFSHQD ARNLLRYGGM RPDRDWLQFD CALSYGLVEY
LRTLEVLRQF GWSPSRCIPH GGHQMSLNIA AGLGLGGNES YPDLFQPYGG FPDGVRVEDG
HITMPDLPGI GFEGKSDLIK VMRELAE
//
