ID A0A3B0B307_9MICO Unreviewed; 546 AA.
AC A0A3B0B307;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN ORFNames=D7252_00425 {ECO:0000313|EMBL:RKN66218.1};
OS Microbacterium sp. CGR2.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=1805820 {ECO:0000313|EMBL:RKN66218.1, ECO:0000313|Proteomes:UP000278458};
RN [1] {ECO:0000313|EMBL:RKN66218.1, ECO:0000313|Proteomes:UP000278458}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGR2 {ECO:0000313|EMBL:RKN66218.1,
RC ECO:0000313|Proteomes:UP000278458};
RA Mandakovic D., Cintolesi A., Maldonado J., Cortes M.P., Mendoza S.,
RA Aite M., Gaete A., Saitua F., Cambiazo V., Agosin E., Siegel A., Maass A.,
RA Gonzalez M., Latorre M.;
RT "Comparative genome-scale metabolic modeling of two Microbacterium species
RT isolated from Atacama Desert.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC {ECO:0000256|ARBA:ARBA00006637}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKN66218.1}.
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DR EMBL; RBHX01000001; RKN66218.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B0B307; -.
DR OrthoDB; 3713880at2; -.
DR Proteomes; UP000278458; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR CDD; cd18789; SF2_C_XPB; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR032438; ERCC3_RAD25_C.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR032830; XPB/Ssl2_N.
DR PANTHER; PTHR11274:SF0; GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB; 1.
DR PANTHER; PTHR11274; RAD25/XP-B DNA REPAIR HELICASE; 1.
DR Pfam; PF16203; ERCC3_RAD25_C; 1.
DR Pfam; PF13625; Helicase_C_3; 1.
DR Pfam; PF04851; ResIII; 1.
DR PRINTS; PR00851; XRODRMPGMNTB.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000313|EMBL:RKN66218.1};
KW Helicase {ECO:0000313|EMBL:RKN66218.1};
KW Hydrolase {ECO:0000313|EMBL:RKN66218.1};
KW Nucleotide-binding {ECO:0000313|EMBL:RKN66218.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000278458}.
FT DOMAIN 190..344
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 399..544
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 546 AA; 60284 MW; EBEEEAB933D2608B CRC64;
MSDGPLIVQS DRTVLLEVAH ADAESARHEL AIFAELERAP EHIHTYRITR LGLWNARAAG
HTAEDMLETL NRWSRFPVPP SVAVDLRETV TRYGRLVIER DDEGTLILRS SDPAVLAQVA
HNKRIQPLLI GHPSPDTFVV DAWARGQIKQ ELLKIGWPAE DLAGYTPGTP HEIDLAEDGW
HIRPYQQDAV DAFSKDGSGV VVLPCGAGKT IVGAGAMAAT KTTTLILVTN TVSARQWRDE
LLKRTSLTAE EIGEYSGQAK EVKPVTIATY QILTAKRKGE YAHLALLDAL DWGLIVYDEV
HLLPAPVFKL TADLQARRRI GLTATLVRED GREGDVFSLI GPKRFDAPWK QIEAQGFISP
AACYEVRVDL PPSDRLEYAA ATDDERYRLA ASAPAKVQAV RELIAKHEGE RILVIGQYLD
QLETLSTALD APQITGATPI DEREELYRAF REGDISLLVV SKVANFSIDL PEASVAIQVS
GSFGSRQEEA QRLGRLLRPK ESGHTASFYT LVARDTIDQD YAQNRQRFLA EQGYSYTIMD
ADAIAA
//
