ID A0A3B0B807_9MICO Unreviewed; 337 AA.
AC A0A3B0B807;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
GN ORFNames=D7252_13180 {ECO:0000313|EMBL:RKN68441.1};
OS Microbacterium sp. CGR2.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=1805820 {ECO:0000313|EMBL:RKN68441.1, ECO:0000313|Proteomes:UP000278458};
RN [1] {ECO:0000313|EMBL:RKN68441.1, ECO:0000313|Proteomes:UP000278458}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGR2 {ECO:0000313|EMBL:RKN68441.1,
RC ECO:0000313|Proteomes:UP000278458};
RA Mandakovic D., Cintolesi A., Maldonado J., Cortes M.P., Mendoza S.,
RA Aite M., Gaete A., Saitua F., Cambiazo V., Agosin E., Siegel A., Maass A.,
RA Gonzalez M., Latorre M.;
RT "Comparative genome-scale metabolic modeling of two Microbacterium species
RT isolated from Atacama Desert.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IB topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00006645}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKN68441.1}.
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DR EMBL; RBHX01000001; RKN68441.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B0B807; -.
DR OrthoDB; 9778962at2; -.
DR Proteomes; UP000278458; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:InterPro.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR Gene3D; 1.10.132.120; -; 1.
DR Gene3D; 3.30.66.10; DNA topoisomerase I domain; 1.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR035447; DNA_topo_I_N_sf.
DR InterPro; IPR049331; Top1B_N_bact.
DR InterPro; IPR001631; TopoI.
DR InterPro; IPR014711; TopoI_cat_a-hlx-sub_euk.
DR InterPro; IPR013500; TopoI_cat_euk.
DR Pfam; PF21338; Top1B_N_bact; 1.
DR Pfam; PF01028; Topoisom_I; 1.
DR PRINTS; PR00416; EUTPISMRASEI.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR SUPFAM; SSF55869; DNA topoisomerase I domain; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:RKN68441.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000278458};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 23..70
FT /note="DNA topoisomerase IB N-terminal"
FT /evidence="ECO:0000259|Pfam:PF21338"
FT DOMAIN 86..285
FT /note="DNA topoisomerase I catalytic core eukaryotic-type"
FT /evidence="ECO:0000259|Pfam:PF01028"
SQ SEQUENCE 337 AA; 37019 MW; 27E89F631618489A CRC64;
MAALTRVVPG EDPGFRRRRS GSGFRYLDHQ DVPAPEADRE RIRDLAIPPA WTDVWIATDP
LAHIQAVGVD GAGRKQYLYH PLWSVSRDRR KFARALDLAA ALPSARAQVT RTLREEGQTK
DRALAIAFRL LDDAALRIGS DRYLVRHGSR GLTTLRRRDV RVEGSDLALS FPAKSGRTAS
IEITDESLAE ALAEFAVGAP RAFLLAYRKG RRRVRITPAE VNTYIRDITG ASFSAKDFRT
LHGTILAADA LARIGRLDSR GERNQAERLA VQAAAAALGN TTAVARGSYI DPRVFRLYSR
GKTMDLTVTP ETAIRRLLGG PEKIGKVDGN SATRRSG
//
