ID A0A3B1JVF8_ASTMX Unreviewed; 1563 AA.
AC A0A3B1JVF8;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=F-actin monooxygenase {ECO:0000256|ARBA:ARBA00012709};
DE EC=1.14.13.225 {ECO:0000256|ARBA:ARBA00012709};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Characidae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000045711.1, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000045711.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + L-methionyl-[F-actin] + NADPH + O2 = H2O + L-methionyl-
CC (R)-S-oxide-[F-actin] + NADP(+); Xref=Rhea:RHEA:51308, Rhea:RHEA-
CC COMP:12953, Rhea:RHEA-COMP:12956, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16044,
CC ChEBI:CHEBI:45764, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.14.13.225; Evidence={ECO:0000256|ARBA:ARBA00001591};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the Mical family.
CC {ECO:0000256|ARBA:ARBA00008223}.
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DR Ensembl; ENSAMXT00000036152.1; ENSAMXP00000045711.1; ENSAMXG00000013076.2.
DR GeneTree; ENSGT00940000158780; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000013076; Expressed in muscle tissue and 13 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR CDD; cd09439; LIM_Mical; 1.
DR Gene3D; 1.10.418.10; Calponin-like domain; 1.
DR Gene3D; 2.10.110.10; Cysteine Rich Protein; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR022735; bMERB_dom.
DR InterPro; IPR001715; CH_dom.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR001781; Znf_LIM.
DR PANTHER; PTHR23167:SF39; [F-ACTIN]-MONOOXYGENASE MICAL2; 1.
DR PANTHER; PTHR23167; CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED; 1.
DR Pfam; PF12130; bMERB_dom; 1.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF01494; FAD_binding_3; 1.
DR Pfam; PF00412; LIM; 1.
DR PRINTS; PR00420; RNGMNOXGNASE.
DR SMART; SM00033; CH; 1.
DR SMART; SM01203; DUF3585; 1.
DR SMART; SM00132; LIM; 1.
DR SUPFAM; SSF47576; Calponin-homology domain, CH-domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF57716; Glucocorticoid receptor-like (DNA-binding domain); 2.
DR PROSITE; PS51848; BMERB; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 1.
DR PROSITE; PS50023; LIM_DOMAIN_2; 1.
PE 3: Inferred from homology;
KW Actin-binding {ECO:0000256|ARBA:ARBA00023203};
KW Coiled coil {ECO:0000256|SAM:Coils}; FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW LIM domain {ECO:0000256|ARBA:ARBA00023038, ECO:0000256|PROSITE-
KW ProRule:PRU00125};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|PROSITE-
KW ProRule:PRU00125}; Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000018467};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PROSITE-ProRule:PRU00125}.
FT DOMAIN 516..619
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 868..930
FT /note="LIM zinc-binding"
FT /evidence="ECO:0000259|PROSITE:PS50023"
FT DOMAIN 1402..1551
FT /note="BMERB"
FT /evidence="ECO:0000259|PROSITE:PS51848"
FT REGION 626..684
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 728..813
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1006..1032
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1072..1207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1226..1341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1353..1393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1412..1439
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 627..656
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 660..684
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 737..751
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 780..805
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1006..1028
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1082..1114
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1123..1207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1243..1257
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1262..1276
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1277..1305
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1563 AA; 175977 MW; 85C3AAA673CE3703 CRC64;
MGETEDERNS QASQLFENFV QASTCKGTLQ AFSVLCRQLE LDPADHQDFY SSLKAAVTSW
KAKALWAKLD KRASHKEYKK GNACPDTRCL IIGGGPCGLR TAIELALLGA KVVVIEKRDT
FSRNNVLHLW PFTIHDLRNL GAKKFYGKFC AGAIDHISIR QLQLILLKVS LIMGVEVHVN
VEFVNLLEPP EDQGNDGIGW RAEIRPDGHP LADYEFDVLI GADGRRSTLE GFKRKEFRGK
LAIAITANFV NRNTTAEAKV EEISGVAFIF NQKFFLELKE ETGVDLENIV YYKDNTHYFV
MTAKKQSLLD KGVIIHDYIE TERLLHSDNV NQEALLSYAR EAADFGTNYQ LPSLDYAINH
YGQPDVAMFD FTCMYASENA ALIREKNGHQ LLVALVGDSL LEPFWPMGTG CARGFLAAFD
TVWMVQGWAQ GKSPLEVLCE RESIYRLLPQ TTAENITKNF EQYTIDPATR YPNLNSSCVR
PHQVRHLFIN GEQNSCMLEH GGPTRRSVNI SRRESEVRPS RLLLWCQKQT QGYRGVDVTD
LTTSWRSGLA LCALIHRQRP DLIDFDSLNE ADCAKNNQLA FEVAERAFGI QPLITGKEMV
AEQEPDKLVM VLYLSKFYEK FRNSSLPVTG APRQTDENNE DYSSKSTNSV YNLMNVSQPR
KRVPKDDKKL DDNDPNKKRR KGYNYLEELS GRDALTAGEG LEQKENKVRS MATQLLAKFE
ENAPSCALRR QVDGSSPAPP APSPPPKPLS VHSLPACSAL SCLLSPPPPP PPQKQCNEDS
ESQQNDHQRN TDRSEIKRIE NLDPSKQRTV GKVSSAIGVK AAALAILYET DHRPNNPVTL
SLTEARRAQD AGSANVRREF APGVGGSDIC HFCKKRVYVM ERLSAEGYFF HRECFRCDVC
SATLRLGGHA FDSDQGTFYC KLHFAQRKTS HRSRKKELPG GSVSLVDGSD GYSATSSLHS
QPSDMLGVRS STVTLRSPVQ NSLQNPSIAT EAPAEERLVT ARIVDSTVQD SPAESESLKP
NSPQLNKVER SYAKGGITNR NISWRKKIRN TLPLILVKTL DRSRIGGQDD ALIEEDSDFE
EIHAPDSPAK SSKHPEDPHC PLQDKQDQAA KSEIPHYRTH GISSPPKPST SSVEQQNEIS
YNSTNQQSPK KKLTLSSSER EKLLNWELAN PTQFSQAAVT TDRTEKNGQV CTEDQPKPQN
DTERSPPLFQ IWANVLRRSF SSSVNNPKVI KRNRPPRARP LSEGSFSFSS LFGASTQFQE
EEEDDNERKR SRAATEGSQS RLKGRSEITT MLEQVSLTTT KPPGGTKDDM ASLPPRKLNF
FSSLRLKRNE GTGRGKTDNQ TKDILSILSK FRNKASTQQQ QHEEDDYSSS EEGPESGAPR
SISDDSERQR KKQEKILIQQ SKREQLKRLH RAQAIQRQLE EVEEKQRVLE EKGVTLEKVL
RGEMNDPSAD EAELLQTWFK LVLEKNKLAR YESELMIFAQ ELELEDRQSQ LQQELRRRMA
VEDCEKSAGE LVEEQALLVE VMKAVEERDR LVSLLEEQRL QEKAEDRDLE GLILSKGYLF
HWA
//
