ID A0A3B2WAS6_MOUSE Unreviewed; 629 AA.
AC A0A3B2WAS6;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Kinetochore protein NDC80 {ECO:0000256|RuleBase:RU368072};
GN Name=Ndc80 {ECO:0000313|Ensembl:ENSMUSP00000156492.2,
GN ECO:0000313|MGI:MGI:1914302};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000156492.2, ECO:0000313|Proteomes:UP000000589};
RN [1] {ECO:0000313|Ensembl:ENSMUSP00000156492.2, ECO:0000313|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000156492.2,
RC ECO:0000313|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0007829|PubMed:21183079}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3] {ECO:0000313|Ensembl:ENSMUSP00000156492.2}
RP IDENTIFICATION.
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000156492.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity. {ECO:0000256|RuleBase:RU368072}.
CC -!- SUBUNIT: Component of the NDC80 complex.
CC {ECO:0000256|RuleBase:RU368072}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore
CC {ECO:0000256|RuleBase:RU368072}. Nucleus
CC {ECO:0000256|RuleBase:RU368072}.
CC -!- SIMILARITY: Belongs to the NDC80/HEC1 family.
CC {ECO:0000256|ARBA:ARBA00007050, ECO:0000256|RuleBase:RU368072}.
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DR AlphaFoldDB; A0A3B2WAS6; -.
DR SMR; A0A3B2WAS6; -.
DR jPOST; A0A3B2WAS6; -.
DR Antibodypedia; 6039; 505 antibodies from 39 providers.
DR Ensembl; ENSMUST00000232863.2; ENSMUSP00000156492.2; ENSMUSG00000024056.11.
DR AGR; MGI:1914302; -.
DR MGI; MGI:1914302; Ndc80.
DR VEuPathDB; HostDB:ENSMUSG00000024056; -.
DR GeneTree; ENSGT00390000018386; -.
DR ChiTaRS; Ndc80; mouse.
DR Proteomes; UP000000589; Chromosome 17.
DR Bgee; ENSMUSG00000024056; Expressed in humerus cartilage element and 135 other cell types or tissues.
DR ExpressionAtlas; A0A3B2WAS6; baseline and differential.
DR GO; GO:0031262; C:Ndc80 complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IEA:UniProtKB-UniRule.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR Gene3D; 6.10.250.1950; -; 1.
DR Gene3D; 1.20.120.560; alix/aip1 in complex with the ypdl late domain; 1.
DR Gene3D; 1.10.418.30; Ncd80 complex, Ncd80 subunit; 1.
DR InterPro; IPR040967; DUF5595.
DR InterPro; IPR005550; Kinetochore_Ndc80.
DR InterPro; IPR038273; Ndc80_sf.
DR PANTHER; PTHR10643; KINETOCHORE PROTEIN NDC80; 1.
DR PANTHER; PTHR10643:SF2; KINETOCHORE PROTEIN NDC80 HOMOLOG; 1.
DR Pfam; PF18077; DUF5595; 1.
DR Pfam; PF03801; Ndc80_HEC; 1.
PE 1: Evidence at protein level;
KW Cell cycle {ECO:0000256|ARBA:ARBA00023306, ECO:0000256|RuleBase:RU368072};
KW Cell division {ECO:0000256|ARBA:ARBA00022618,
KW ECO:0000256|RuleBase:RU368072};
KW Centromere {ECO:0000256|ARBA:ARBA00023328, ECO:0000256|RuleBase:RU368072};
KW Chromosome {ECO:0000256|ARBA:ARBA00022454, ECO:0000256|RuleBase:RU368072};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Kinetochore {ECO:0000256|ARBA:ARBA00022838, ECO:0000256|RuleBase:RU368072};
KW Mitosis {ECO:0000256|ARBA:ARBA00022776, ECO:0000256|RuleBase:RU368072};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU368072};
KW Proteomics identification {ECO:0007829|EPD:A0A3B2WAS6,
KW ECO:0007829|MaxQB:A0A3B2WAS6};
KW Reference proteome {ECO:0000313|Proteomes:UP000000589}.
FT DOMAIN 213..285
FT /note="DUF5595"
FT /evidence="ECO:0000259|Pfam:PF18077"
FT COILED 240..400
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 458..538
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 629 AA; 72715 MW; 262ED0A76F39EBCC CRC64;
MKRSSVSTCG AGRLSMQELR TLDLNKPGLY TPQTKERSTF GKLSTHKPTS ERKVSIFGKR
TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLY EFLTENGYVY SVSMKSLQAP
STKEFLKIFA FLYGFLCPSY ELPGTKCEEE VPRIFKALGY PFTLSKSSMY TVGAPHTWPH
IVAALVWLID CIKIDTAMKE SSPLFDDGQL WGEETEDGIK HNKLFLEYTK KCYEKFMTGA
DSFEEEDAEL QAKLKDLYKV DASKLESLEA ENKALNEQIA RLEEEREREP NRLMSLKKLK
ASLQADVQNY KAYMSNLESH LAVLKQKSNS LDEEIGRVEQ ECETVKQENT RLQSIVDNQK
YSVADIERIN HEKNELQQTI NKLTKDLEAE QQQMWNEELK YARGKEAIEA QLAEYHKLAR
KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRTQVYAP LKELLNESEE EINKALNKKR
HLEDTLEQLN TMKTESKNTV RMLKEEIQKL DDLHQQAVKE AEEKDKKSAS ELESLEKHKH
LLESGVNDGL SEAMDELDAV QRENILKRRM LKPTESMKNS CLKISWKTSG RWQRSIREML
PNLRLPTNED KLIFHRYNIV FYPQIMIIW
//
