ID A0A3B3C211_ORYME Unreviewed; 2616 AA.
AC A0A3B3C211;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE SubName: Full=DNA polymerase theta {ECO:0000313|Ensembl:ENSOMEP00000011619.1};
GN Name=POLQ {ECO:0000313|Ensembl:ENSOMEP00000011619.1};
OS Oryzias melastigma (Marine medaka).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=30732 {ECO:0000313|Ensembl:ENSOMEP00000011619.1, ECO:0000313|Proteomes:UP000261560};
RN [1] {ECO:0000313|Ensembl:ENSOMEP00000011619.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
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DR STRING; 30732.ENSOMEP00000011619; -.
DR PaxDb; 30732-ENSOMEP00000011619; -.
DR Ensembl; ENSOMET00000032387.1; ENSOMEP00000011619.1; ENSOMEG00000012947.1.
DR GeneTree; ENSGT00940000158694; -.
DR OMA; HNMCQQF; -.
DR OrthoDB; 208079at2759; -.
DR Proteomes; UP000261560; Unplaced.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:UniProt.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR CDD; cd18026; DEXHc_POLQ-like; 1.
DR CDD; cd08638; DNA_pol_A_theta; 1.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR Gene3D; 1.10.3380.20; -; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR046931; HTH_61.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR048960; POLQ-like_helical.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF20470; HTH_61; 1.
DR Pfam; PF21099; POLQ_helical; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF158702; Sec63 N-terminal domain-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}.
FT DOMAIN 322..501
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 540..746
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 20..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 164..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1115..1289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1354..1402
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1425..1514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1532..1556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1694..1867
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1925..1951
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2314..2341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..198
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..225
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1117..1131
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1141..1180
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1199..1216
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1241..1255
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1256..1272
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1373..1402
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1434..1467
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1468..1492
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1699..1725
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1764..1812
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1822..1859
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1925..1939
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2616 AA; 289214 MW; 50E12099359E82F0 CRC64;
MTSGPLKKKN YLGQHQIKKK ISININDEPP NRDRLSEKAS HLPDRSIQLP NNAKQEQVLG
ESTIALDEEI LQVFGRLHPE KDTINKHVQE EVQSSDASAP NFAPAACTKK DMDSTQVRVR
ASFAESGPHS ALSPQEEDSR RKRDCKRPGW RAECKELAHK LLFSEDSGNK ENPQKNHSDP
SSDYVKAQEI QSSPDAHNSC KDASRRSRSP LRESSQTSSK PLNASNDYIL FSPRRLAAAM
KRSKLQRSVQ NQSASVLTVP SGLELSTLSD TLPKPGIALC APVEQAEKLL LSSWGLPKPV
LEQYQKYGVT QMFEWQAQCL TVGQVLQGGN LVYSAPTSAG KTLVSELLML KRVLETKRKA
LFILPFVSVA KEKMQYLQSV FQEVGVRVEG YMGSTSAAGG FSKLDVAVCT IEKANSLVNR
LIEEDDMALL GMVVVDELHM VGDSGRGYLL ELLLTKILYI SRKQNTSGSL SEGVQIIGMS
ATLPNLSLLA AWLGAELYQT DYRPVPLEEY LKVDSNIYDK SLSVVRQFSP VLHVKGDDDH
IVSLCYETVR EGHSVLLFCP SKNWCEKLAD SIGREFYNIR GADGKAEPQP VCLDREGLMD
VVAQLRRTPA GLDPILQRTV QWGVGFHHAG LTFDERDVLE GAFRQGLVRV LSATSTLSSG
VNLPARRVII RTPTFNGRLL DPLTYKQMAG RAGRKGVDTK GESLLVCKKS EEKKGATLLR
GSLQPISSCL VRREGEGVTT SMLRAILEII VGGVASTPQD VRLYASCSLL AASMKSDDKK
SSDEESNRGA IEACVEWLME NEFINIQKDE QDERYCPTQL GAATLASSLS PPEALGIFAD
LQRAMKGFVL ENDLHIMYLI TPLYAEWTTI DWYQFFCLWE QLSSSMKRVA ELVGVQESFL
ARSVSGKLVA KTEKQRRQMA IHKRFFTTLV LQDLVSEVPL GTVASKYNCN RGQLQSLQQS
ASTYAGMVTV FCKRLGWHNM ELLLSQYQTR LSFGVQRELV DLVRVSLLNA TRARALYAQG
LCTVAELARG TVADVEKALR NSVPFKSSKR AVDESEVEAA ERRSLRCVWV AGGRALTEQE
AAVEIVSEAR LLLQEDLARL GVQWDPAALP AESCIPDGHS SSDSDTSPAS CLSPPVVKAH
SSKGHHKEMD NRVTEDRKGV KRMVKGEDSN ELDVREKNNK KMRAKTNKPI QNYSENPDKT
VGNKHEEERE KAEQGETGST TGKPEGLHSK PPAPERSLTQ ELADIVSSLS PHLLQPPQPS
FSPSPAPPPR FRAPISRGDG SIAEEEGLTK LSTSPLQLER LKHSKALSKV LQSIQCIHDS
VNPSEKAGSK PTVISNLCNS EPPVEEIKAA QTFGHAQEDA SVPSAFPGSV DRCSPEAKRR
KIDAEDAEKF SSPELYAGDD RDVDVQASFG DSFELDTQTE RIIVQQTHRN SDERSGGTNQ
HIREEERVEA AGKQVQTVDE ERNDRETPGC NISLTDSQME HILNTSHPKS PSPAAGDDTE
KSDNPASESA NRSSSLLFDS LYDSPLIAAL RAEQSDEDDS APAEAECPLP TTQQRRRSEL
LANQEAEEQE AVRWGESSFN LSEWGDSLLV GEHFLERQNV LRFVEQTGQQ VIKNHDSCDA
LELCQKQLLL KAQNEPGEDQ SRNMMQLNNE EQRDDQERKS DEKEINVFLT NNENCKHPHQ
QDLYCSPGLQ EIFDRWPSMS DQPGSSTSGI QAGTANAAMH PQTDAAKTET QKERLSGCES
ENVMQRPGSA GDLIPPTQER APVTPRVKLT TSSIQSQGST PRPLKQSTPS NPSEATLGNG
HFLSAVASSS KKQEKPSPKK ELKSNPQQSS KTKAKQQTDQ NVQLPAQSTS PSSPGPRSPS
DAESPEIPEG FTLQLSQDET LCSSNSGTFS IIDVASDRRL FETFTKEWKT KERFALALAL
EKREHGQQQG EEIGGKHRRA STANQKLNRP DGFPVRNDEG LVLIGLSVCW GARDAYYISL
QEEQSKGLSS SLAPPPLDDD LAVTERLEQV KVCLGGQPAG NKVHDVVAYD IIRVYKRLVL
SCGISLEGNC EDPMVACWLL DPSSEERTIS NMVTVYCPEE LPLLEGLGNS HSYCPRVRAA
TSSVLIHAVM NHLTGLLEED GTLDLFRNVE MPSQVCLALL ELNGLGFSVE ECERQKHVMQ
AKLSALENEA YSLAGHSFSL TSVDDIAQVL FLELHLPPNG EIGEPRTKKT LGYTRRGGGK
GRLGKQFSTT KDVLEKLRPL HPLPGVILEW RRITNALTKV VFPLQREKQY HPKLKMDRIY
PTAQTHTATG RVSFTEPNIQ NVPKDFDIEM PTLVKESPPS QNAPHRPGKK RRSVTAAAAR
GPAEQGPAFS VSMRHAFIPF SGGMILAADY SQLELRVLAH LSKDQRLLQV LNGGADVFRC
IAAEWKNVDP ESVQDDLRQQ AKQICYGIIY GMGAKSLGEQ MGVEENDAAC YIESFKDRYK
GISAFLKQTV KNCIKDGFVQ TLMSRRRYLP GIISSNPHIR AHAERQAVNT TVQGSAADIV
KLATVNIQKR LRETYPGVPP SHQHLHTARN QRRAVRGAYF ILQLHDELIY ETTEQDLIQV
AQIVKREMES AVKLYVKLKV KVKMGSSWGD LQDIDI
//
