ID A0A3B3CAG7_ORYME Unreviewed; 862 AA.
AC A0A3B3CAG7;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=E3 ubiquitin-protein ligase {ECO:0000256|PIRNR:PIRNR001569};
DE EC=2.3.2.26 {ECO:0000256|PIRNR:PIRNR001569};
OS Oryzias melastigma (Marine medaka).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=30732 {ECO:0000313|Ensembl:ENSOMEP00000014800.1, ECO:0000313|Proteomes:UP000261560};
RN [1] {ECO:0000313|Ensembl:ENSOMEP00000014800.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885,
CC ECO:0000256|PIRNR:PIRNR001569};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|PIRNR:PIRNR001569}.
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DR AlphaFoldDB; A0A3B3CAG7; -.
DR STRING; 30732.ENSOMEP00000014800; -.
DR PaxDb; 30732-ENSOMEP00000014800; -.
DR Ensembl; ENSOMET00000022796.1; ENSOMEP00000014800.1; ENSOMEG00000016326.1.
DR GeneTree; ENSGT00940000157014; -.
DR OMA; KRTDING; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000261560; Unplaced.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR CDD; cd04021; C2_E3_ubiquitin_ligase; 1.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd00201; WW; 4.
DR Gene3D; 2.20.70.10; -; 3.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR024928; E3_ub_ligase_SMURF1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR11254:SF445; E3 UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00397; WW; 4.
DR PIRSF; PIRSF001569; E3_ub_ligase_SMURF1; 1.
DR SMART; SM00239; C2; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00456; WW; 4.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF51045; WW domain; 4.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS01159; WW_DOMAIN_1; 4.
DR PROSITE; PS50020; WW_DOMAIN_2; 4.
PE 4: Predicted;
KW Transferase {ECO:0000256|PIRNR:PIRNR001569};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PIRNR:PIRNR001569}.
FT DOMAIN 1..100
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 284..317
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 316..349
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 396..429
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 436..469
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 528..862
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 144..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..217
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 224..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 830
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001569-1,
FT ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 862 AA; 98847 MW; 5D563B718D1A7DE0 CRC64;
MRAQLQVTIL SAKLKENKKN WFGPSPYVEV TVDGQSKRTE KCNNTHSPKW KQSLTVIVSP
VSELFFRVWS HQTLKADILL GMATLDICQI LKANDFKLCE VVQSLQLSTG RNSLDVVGEL
SVCLDGMQVH PEALAQAESE LASLPNGTSR QNGNTINRSS RDSSPSSDSD DWVIVSNDHT
ANGTDSLSTT SRPPPSMPHR SAASASSSSN SSPSELGEAP ASDGFSQTVP SQEESGGGAG
TTVSSQRANP RPQNTATNPS PKPGAATTTA STAATPATSS VNNCPLPPGW EQRVDQNGRV
YYVDHIEKRT TWDRPEPLPS GWERRVDPMG RVYYVDHITR TTTWQRPTQE SVRNYEEWQH
QRSQLQGAMQ QFNQRFIYGL QDQLAAIANK EFDPLGPLPH GWEKRTDTNG RMYFVHHPTR
TTQWEDPRRQ GLLNDKPLPE GWEMRFTVDG IPYFVDHNRR TTTYIDPRTG KSSSLENGPQ
ITYVRDFKAK VQYFRFWCQQ LSMPQHIKIT VSRKTLFEDS FQQIMSFHPQ DLRRRLWIIF
PGEEGLDYGG VAREWFFLLS HEVLNPMYCL FEYAGKDNYR LQINPASYIN PDHLKYFKFI
GRFIAMALFH GKFIDTGFSL PFYKRILNKP LTLKDLESID PEFYNSLIWI KDNNIEECGL
EMFFSVDKEI LGEVTTHELK PDGGNIQVTE ENKEEYIRLV AEWRLSRGVE EQTQAFFEGF
NEVLPQQYLQ YFDAKELEVM LCGMQEIDLA DWQRNTIYRH YARNSKQIMW FWQFVKEMDN
EKRMRLLQFV TGTCRLPMGG FGDLMGSNGP QKFCIEKVGK ENWLPRSHTC FNRLDLPPYK
SYEQLKEKLM FAIEETEGFG QE
//
