ID A0A3B3CR57_ORYME Unreviewed; 1611 AA.
AC A0A3B3CR57;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=HECW1 {ECO:0000313|Ensembl:ENSOMEP00000020363.1};
OS Oryzias melastigma (Marine medaka).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=30732 {ECO:0000313|Ensembl:ENSOMEP00000020363.1, ECO:0000313|Proteomes:UP000261560};
RN [1] {ECO:0000313|Ensembl:ENSOMEP00000020363.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR STRING; 30732.ENSOMEP00000020363; -.
DR PaxDb; 30732-ENSOMEP00000020363; -.
DR Ensembl; ENSOMET00000036074.1; ENSOMEP00000020363.1; ENSOMEG00000022347.1.
DR GeneTree; ENSGT00940000158294; -.
DR OrthoDB; 5480520at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000261560; Unplaced.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd08691; C2_NEDL1-like; 1.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd00201; WW; 2.
DR Gene3D; 2.20.70.10; -; 2.
DR Gene3D; 2.60.40.2840; -; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037795; C2_HECW.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR040524; HECW1_helix.
DR InterPro; IPR032348; HECW_N.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR11254:SF79; E3 UBIQUITIN-PROTEIN LIGASE HECW1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF18436; HECW1_helix; 1.
DR Pfam; PF16562; HECW_N; 1.
DR Pfam; PF00397; WW; 2.
DR SMART; SM00239; C2; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00456; WW; 2.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF51045; WW domain; 2.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS01159; WW_DOMAIN_1; 2.
DR PROSITE; PS50020; WW_DOMAIN_2; 2.
PE 4: Predicted;
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 185..318
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 856..889
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 1023..1056
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 1276..1611
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 368..555
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 585..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 757..837
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 885..908
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 920..947
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1202..1224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 399..441
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 525..550
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 588..615
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 932..947
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1205..1219
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1579
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 1611 AA; 180028 MW; 47C1F233325EF297 CRC64;
MIHRQVLLPQ NLYQNRLLSL AAMASPTRSG PTRQRCKDVA RHSYGAETYN VNGLNQEAFL
LGLSRSTSDT DLVSPDARST LTVSSSHYTI GQSEDLVISW DIKEEVDAGD WIGMYQVDEV
LSENFLDYKN RGISGSHKGQ IVWKIESGAN FSDVETQVCF RYYHGVTGAL RATTPSVTIK
KSAPPVSKPV VSPEVNHGMG NRRLINFWLS DLQAVGLKKG MFFNPDPYLK LSIQPGKHSI
FPSLPHHGQE KRSRVVCNTI NPQWTTERFN FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK
LSVPVQRLLE KHAIGDRVVS YSLGRRVPTD HVCGQLLFRF ELTSSIHPDD EEVSLVIETA
CAEAANAADD AGGAADDDTL SVGPDMPNLP LDAPPEAENS PPTHEEETHP AEREEEEGGA
QVEQGGTEEE EDRREEPPAI QQQEENEEQQ EANGIQEEGG EARAEEEQRQ EVAPQSPAVE
SKDGDEEELG ASSGGAAAVE APSEESATGA GEGAPKGETE GGERSSPPCS CQPPSSNLNS
NASSRRKNRP CSLPVSELET VIASACGEPE TPRSHYIRIH HLLHSLPSAQ QRAPSQEGED
TSSTVDATSP TLKTSKEENG PEEEEEDDDT TQSPSQVPEC PGPCCRRSLP RSLSIERLSE
LNQLLEGDGG RGHPVMRRIS PSCLDSEEGD SSNGGRRVCG GTHRPLGENE CEFCDTSCYS
TSCYSTSCYS TSCYSNSGFE GRGRFCSHNR LSSVDSNRLS GSTVFSSQDE EDEEESAFES
VPDFAHPEGQ NLGGPEGGRT PPSGCKENRR GEQGEPAVAG PSDRNGIGSD SSPPVDHLPV
LRPSHDLNHF PAATDQALPP NWEARIDSHG RVFYVDHVNR TTTWQRPSHS TKCNHGIPRS
SSTQQMEQLN RRYQNIQRTM ATEEDGGSRS SDGDSDSPQT GPSSPVNQQK ITHLLQSPAV
KFVTHPEFFT VLHSNYAAYR MFTSSSCVKH MVLKVRRDAR NFERYQHNRD LVVFINKFAD
TQLELPRGWE IKTDPQGKSF FVDHNSRATT FIDPRIPLQN GRLPGHLAHK QHLQRLRSYS
AGEASEVSRS RGASLMVRPG NSLVAAIRSQ HQPDSQPLSA PSYNDKIVAF LRQPNIFDML
QERQPSLSRN HALREKIHYI RTEGTQGVEK LSCDADLVIL LSLFEEEIMS YAPPHPIHPG
FSFSPRCSPA SSPQNSPGLQ RARAPAPYRR DFEAKLRNFY RKLEAKGYGQ GPGKIKLLIR
REHLLEGTFN QVMAYSRKEL QRNKLYVTFL GEEGLDYSGP SREFFFLLSQ ELFNPYYGLF
EYSANDTYTV QISPMSAFVE NHLEWFRFSG RILGLALIHQ YLLDAFFTRP FYKALLRLPT
DLSDLEYLDE EFHQSLQWMK DNDITDILEL TFTVNEEVFG QVTERELKSG GSNLQVTEKN
KKDYIERMTK WRVERGVVQQ TEALVRGFYE VVDSRLVSVF DARELELVIA GTVEIDLNDW
RSNTEYRGGY HDGHIVMRWF WAAVERFNNE QRLRLLQFVT GTSSVPYEGF AALRGSNGLR
RFCIEKWGKV TSLPRAHTCF NRLDLPPYPS YTILYEKLLI AVEETSTFGL E
//
