ID A0A3B3DH15_ORYME Unreviewed; 938 AA.
AC A0A3B3DH15;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Aminopeptidase {ECO:0000256|RuleBase:RU364040};
DE EC=3.4.11.- {ECO:0000256|RuleBase:RU364040};
OS Oryzias melastigma (Marine medaka).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=30732 {ECO:0000313|Ensembl:ENSOMEP00000029398.1, ECO:0000313|Proteomes:UP000261560};
RN [1] {ECO:0000313|Ensembl:ENSOMEP00000029398.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3,
CC ECO:0000256|RuleBase:RU364040};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3,
CC ECO:0000256|RuleBase:RU364040};
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136, ECO:0000256|RuleBase:RU364040}.
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DR AlphaFoldDB; A0A3B3DH15; -.
DR Ensembl; ENSOMET00000018840.1; ENSOMEP00000029398.1; ENSOMEG00000013036.1.
DR GeneTree; ENSGT00940000159086; -.
DR OMA; MENWGVV; -.
DR Proteomes; UP000261560; Unplaced.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 1.
DR Gene3D; 1.25.50.20; -; 1.
DR Gene3D; 2.60.40.1910; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR11533:SF156; ENDOPLASMIC RETICULUM AMINOPEPTIDASE 1; 1.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000256|RuleBase:RU364040};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU364040};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU364040};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU364040};
KW Signal {ECO:0000256|SAM:SignalP};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT SIGNAL 1..17
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 18..938
FT /note="Aminopeptidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5017206633"
FT DOMAIN 51..233
FT /note="Aminopeptidase N-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF17900"
FT DOMAIN 268..474
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 589..909
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT ACT_SITE 341
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 340
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 344
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 363
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 425
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 938 AA; 107630 MW; 62C718AD81C22B51 CRC64;
MFPLLLLIFT LFPSSPGAQI PNQDQTKEIP IATNGQLFPW DRMRLPKIIS PLHYDLSLHP
NLTTLDFTGV VRIDLDVHED TSVVILHAKK MHISGALLLA PEGVRPLQVL EYPRFHQLAL
MSDLVLTKGR KYEVQLEFAA NLSDSYHGFY KGSYRTSSGE VRVLASTQFE ATFARGAFPC
FDEPAFKANF TIRIIREPRH IAISNMPKVK TVELPGDLLQ DHFDTTVKMS TYLVAYIVSD
FLSVSKTTQH GVKISIYAVP DKINQTAFAL DAAVKLLDFY DDYFDIPYPL PKQDLAAIPD
FQSGAMENWG LTTYRETGLL FDPDKSSASD KLGITKVIAH ELAHQWFGNL VTMEWWNDLW
LNEGFAKFME FVSLDITYPE LQVDDFFLGK CFEAMEVDSL SSSHPVSTPV ENPTQIQEMF
DDVSYDKGAC ILNMLRDFLT PEAFEIGIIR YLKRFSYQNT VNSHLWESLT NICSTDDLDE
GRMKHTEFCS KRKLNSGSSK WYSGDELDVK AIMDTWTLQE GFPLVTVEVK GREVRLSQER
FLRTDNPSLT EGFLWQIPLT YMTSASSSVH RFLLKTKSDV LYLPEEVDWV KFNVNMSGYY
MVHYGAEGWN SIISLLHHNH TALSGNDRAS LIHNVFQLVS VEKVRLDTAL ELSLYLSKET
AIMAVWQGFG ELVPLYKLME KRDMAELENQ MKSFIVDLFR GLIDQQEWSD SGSVSQRVLR
SYLLLFACVR NYSPCVTRAT QLFNQWRDSD GTMSLPVDIT MAVFVVGART RDGWDFMYEK
YRTSLQMSVK SRMKSAMAVT TMKDKLQWMM EQSLNGEVMK TQDLPDVIVS VARNPHGFKL
AWDFFRAHWH TLIQKFDLGS STLSYLVGGV TNQYSTREKL HEIRSFFDSL TEETGSKMRC
IQQSYETIED NIRWMDRNLP LLQAWLNKHG SRERHEEL
//
