ID A0A3B3DRJ1_ORYME Unreviewed; 605 AA.
AC A0A3B3DRJ1;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=t-plasminogen activator {ECO:0000256|ARBA:ARBA00013193};
DE EC=3.4.21.68 {ECO:0000256|ARBA:ARBA00013193};
GN Name=PLAT {ECO:0000313|Ensembl:ENSOMEP00000032049.1};
OS Oryzias melastigma (Marine medaka).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=30732 {ECO:0000313|Ensembl:ENSOMEP00000032049.1, ECO:0000313|Proteomes:UP000261560};
RN [1] {ECO:0000313|Ensembl:ENSOMEP00000032049.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Specific cleavage of Arg-|-Val bond in plasminogen to form
CC plasmin.; EC=3.4.21.68; Evidence={ECO:0000256|ARBA:ARBA00001538};
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00076}.
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DR AlphaFoldDB; A0A3B3DRJ1; -.
DR Ensembl; ENSOMET00000023883.1; ENSOMEP00000032049.1; ENSOMEG00000017298.1.
DR GeneTree; ENSGT00940000158930; -.
DR OMA; WCYIFKA; -.
DR Proteomes; UP000261560; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0048856; P:anatomical structure development; IEA:UniProt.
DR GO; GO:0007586; P:digestion; IEA:UniProtKB-KW.
DR GO; GO:0031639; P:plasminogen activation; IEA:InterPro.
DR CDD; cd00054; EGF_CA; 1.
DR CDD; cd00108; KR; 2.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.10.70.10; Complement Module, domain 1; 1.
DR Gene3D; 2.10.25.10; Laminin; 1.
DR Gene3D; 2.40.20.10; Plasminogen Kringle 4; 2.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 3.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR000001; Kringle.
DR InterPro; IPR013806; Kringle-like.
DR InterPro; IPR018056; Kringle_CS.
DR InterPro; IPR038178; Kringle_sf.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR026280; Tissue_plasm_act.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR PANTHER; PTHR24264:SF42; TISSUE-TYPE PLASMINOGEN ACTIVATOR; 1.
DR PANTHER; PTHR24264; TRYPSIN-RELATED; 1.
DR Pfam; PF00008; EGF; 1.
DR Pfam; PF00051; Kringle; 2.
DR Pfam; PF00089; Trypsin; 2.
DR PIRSF; PIRSF001145; Tissue_plasm_act; 2.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR PRINTS; PR00018; KRINGLE.
DR SMART; SM00181; EGF; 1.
DR SMART; SM00130; KR; 2.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF57603; FnI-like domain; 1.
DR SUPFAM; SSF57440; Kringle-like; 2.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS00022; EGF_1; 1.
DR PROSITE; PS01186; EGF_2; 1.
DR PROSITE; PS50026; EGF_3; 1.
DR PROSITE; PS00021; KRINGLE_1; 2.
DR PROSITE; PS50070; KRINGLE_2; 2.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
PE 4: Predicted;
KW Digestion {ECO:0000256|ARBA:ARBA00022757};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157,
KW ECO:0000256|PIRSR:PIRSR001145-3};
KW EGF-like domain {ECO:0000256|ARBA:ARBA00022536, ECO:0000256|PROSITE-
KW ProRule:PRU00076};
KW Kringle {ECO:0000256|ARBA:ARBA00022572, ECO:0000256|PROSITE-
KW ProRule:PRU00121}; Plasminogen activation {ECO:0000256|ARBA:ARBA00023202};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..605
FT /note="t-plasminogen activator"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5017238717"
FT DOMAIN 78..116
FT /note="EGF-like"
FT /evidence="ECO:0000259|PROSITE:PS50026"
FT DOMAIN 122..204
FT /note="Kringle"
FT /evidence="ECO:0000259|PROSITE:PS50070"
FT DOMAIN 211..293
FT /note="Kringle"
FT /evidence="ECO:0000259|PROSITE:PS50070"
FT DOMAIN 331..601
FT /note="Peptidase S1"
FT /evidence="ECO:0000259|PROSITE:PS50240"
FT ACT_SITE 377
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-1"
FT ACT_SITE 426
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-1"
FT ACT_SITE 553
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-1"
FT DISULFID 38..66
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 64..74
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 82..93
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 87..104
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3,
FT ECO:0000256|PROSITE-ProRule:PRU00076"
FT DISULFID 106..115
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3,
FT ECO:0000256|PROSITE-ProRule:PRU00076"
FT DISULFID 123..204
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 144..186
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 175..199
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 212..293
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 233..275
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 264..288
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 316..450
FT /note="Interchain (between A and B chains)"
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 362..378
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 370..439
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 464..559
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
FT DISULFID 496..512
FT /evidence="ECO:0000256|PIRSR:PIRSR001145-3"
SQ SEQUENCE 605 AA; 67945 MW; 1C78B33691BCF1CB CRC64;
MLRSLHLFLL LSALYCSLAE NVELLRSKRG VRFYRVLCVD SETSAVRSYG DTWLRWKGQR
VEYCHCALRG QERCHSVPVI NCYVSQCYNG GTCKESVYTS DYICQCPQGF KGTQCEINSN
EKCIVGKGEG YRGTLSVTRS GEECINWNST SLRGKKFTAR KNDASSLGLG NHNFCRNPDQ
DSSPWCYIYK GNQIIWELCS LPKCSGDRNR ECMQGLGKSY RGTTAVTKTG SACLPWDSPA
VRRKRNNAWK SNALDLGLSN HNFCRNPDGD SGPWCHTYKN MQLTWELCDI PKCSRAPSTI
TTLGPRAPTT NNQATCGQRL DNTLNRPTFR IFGGRGSDIT EQPWQAAINF YVPRVKRHFH
LCGGVLIDSC WVLSAAHCFQ EKDTPEILQV ILGRTFRTQN SNSEQIFKVE KLWIHEKFDS
ETYDNDIAIL KLKTDMGLCA VNSEEVLPAC LPDRGLQLPD WTECEISGYG KSSEFSPEFS
EQVKRGHVRL WPKERCTPEV LSGRPVTPNM LCAGDTRGKD DACKVSVSTL IKPQSLMSLP
IIIPSSFYSQ GDSGGPLVCR NQNRMTLMGL VSWGDGCGEK DKPGVYTRVT NYIDWIKRKI
QASPV
//
