ID A0A3B3YAC6_9TELE Unreviewed; 624 AA.
AC A0A3B3YAC6;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Phosphoenolpyruvate carboxykinase, cytosolic [GTP] {ECO:0000256|ARBA:ARBA00040372};
DE EC=4.1.1.32 {ECO:0000256|ARBA:ARBA00012306};
DE AltName: Full=Serine-protein kinase PCK1 {ECO:0000256|ARBA:ARBA00042054};
OS Poecilia mexicana.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Cyprinodontiformes; Poeciliidae; Poeciliinae;
OC Poecilia.
OX NCBI_TaxID=48701 {ECO:0000313|Ensembl:ENSPMEP00000024108.1, ECO:0000313|Proteomes:UP000261480};
RN [1] {ECO:0000313|Ensembl:ENSPMEP00000024108.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + L-seryl-[protein] = GDP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:64020, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:58189, ChEBI:CHEBI:83421;
CC Evidence={ECO:0000256|ARBA:ARBA00036764};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64021;
CC Evidence={ECO:0000256|ARBA:ARBA00036764};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:10388, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:37565, ChEBI:CHEBI:58189, ChEBI:CHEBI:58702; EC=4.1.1.32;
CC Evidence={ECO:0000256|ARBA:ARBA00035922};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10389;
CC Evidence={ECO:0000256|ARBA:ARBA00035922};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:10390;
CC Evidence={ECO:0000256|ARBA:ARBA00035922};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC {ECO:0000256|ARBA:ARBA00004742}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC family. {ECO:0000256|ARBA:ARBA00005796}.
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DR RefSeq; XP_014857706.1; XM_015002220.1.
DR RefSeq; XP_014857707.1; XM_015002221.1.
DR AlphaFoldDB; A0A3B3YAC6; -.
DR STRING; 48701.ENSPMEP00000024108; -.
DR Ensembl; ENSPMET00000008474.1; ENSPMEP00000024108.1; ENSPMEG00000006183.1.
DR GeneID; 106926981; -.
DR KEGG; pmei:106926981; -.
DR CTD; 5105; -.
DR OrthoDB; 5471652at2759; -.
DR UniPathway; UPA00138; -.
DR Proteomes; UP000261480; Unplaced.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:UniProtKB-EC.
DR GO; GO:0043648; P:dicarboxylic acid metabolic process; IEA:UniProt.
DR GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR CDD; cd00819; PEPCK_GTP; 1.
DR Gene3D; 3.90.228.20; -; 1.
DR Gene3D; 3.40.449.10; Phosphoenolpyruvate Carboxykinase, domain 1; 1.
DR Gene3D; 2.170.8.10; Phosphoenolpyruvate Carboxykinase, domain 2; 1.
DR HAMAP; MF_00452; PEPCK_GTP; 1.
DR InterPro; IPR018091; PEP_carboxykin_GTP_CS.
DR InterPro; IPR013035; PEP_carboxykinase_C.
DR InterPro; IPR008209; PEP_carboxykinase_GTP.
DR InterPro; IPR035077; PEP_carboxykinase_GTP_C.
DR InterPro; IPR035078; PEP_carboxykinase_GTP_N.
DR InterPro; IPR008210; PEP_carboxykinase_N.
DR PANTHER; PTHR11561; PHOSPHOENOLPYRUVATE CARBOXYKINASE; 1.
DR PANTHER; PTHR11561:SF19; PHOSPHOENOLPYRUVATE CARBOXYKINASE, CYTOSOLIC [GTP]; 1.
DR Pfam; PF00821; PEPCK_GTP; 1.
DR Pfam; PF17297; PEPCK_N; 1.
DR PIRSF; PIRSF001348; PEP_carboxykinase_GTP; 1.
DR SUPFAM; SSF68923; PEP carboxykinase N-terminal domain; 1.
DR SUPFAM; SSF53795; PEP carboxykinase-like; 1.
DR PROSITE; PS00505; PEPCK_GTP; 1.
PE 3: Inferred from homology;
KW Decarboxylase {ECO:0000256|ARBA:ARBA00022793};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 29..257
FT /note="Phosphoenolpyruvate carboxykinase GTP-utilising N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF17297"
FT DOMAIN 261..620
FT /note="Phosphoenolpyruvate carboxykinase C-terminal P-loop"
FT /evidence="ECO:0000259|Pfam:PF00821"
SQ SEQUENCE 624 AA; 69367 MW; CA7E25D3CF728169 CRC64;
MPPQIQSQNQ SGLRVLQGEL SALSPAVREF VETNVTLCQP ESLHICDGSD QENRAILTQL
EEQGMIKKLT KYENCWLART DPGDVARVES KTVIVTREQR ETVPTPLGGG TSQLGRWMSP
EEFDKAMAQR FPGCMKGRTM YVVPFSMGPV GSPLSKIGVE LTDSPYVVAS MRVMTRMGKT
VLNELGNGEF VRCLHSVGCP LPLKKPLVNN WPCNPEQTLV AHIPERRQII SFGSGYGGNS
LLGKKCFALR IASRIAKEEG WLAEHMLILG ITNPAGEKKY MAAAFPSACG KTNLAMLCPT
LPGWKVECVG DDIAWMKFDN QGNLRAINPE NGFFGVAPGT SAKTNPNAMA TIVKNTIFTN
VAETSDGGIY WEGMEQELPE GVTLTSWKNK PWSSEDGEPC AHPNSRFCTP ASQCPIIDPQ
WESPEGVPIE AIIFGGRRPE GVPLVYEAFN WQHGVFVGAT MRSEATAAAE HKGKVIMHDP
FAMRPFFGYN FGQYLSHWLS MADRPGAKLP KIFHVNWFRK SPKAGFLWPG FGDNIRVLDW
MFRRVDGNTG AMPSTVGYLP CNDSLNLQAL EGKVNLSELF SLEHEFWQKE VEEVRKYFTT
QVNDDLPNEM ARQLELLQQR VKQM
//
