ID A0A3B4C448_PYGNA Unreviewed; 5403 AA.
AC A0A3B4C448;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Midasin {ECO:0000256|ARBA:ARBA00017143, ECO:0000256|PIRNR:PIRNR010340};
GN Name=MDN1 {ECO:0000313|Ensembl:ENSPNAP00000006503.1};
OS Pygocentrus nattereri (Red-bellied piranha).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Pygocentrus.
OX NCBI_TaxID=42514 {ECO:0000313|Ensembl:ENSPNAP00000006503.1, ECO:0000313|Proteomes:UP000261440};
RN [1] {ECO:0000313|Ensembl:ENSPNAP00000006503.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}. Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|ARBA:ARBA00007188, ECO:0000256|PIRNR:PIRNR010340}.
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DR STRING; 42514.ENSPNAP00000006503; -.
DR Ensembl; ENSPNAT00000003449.1; ENSPNAP00000006503.1; ENSPNAG00000002433.1.
DR GeneTree; ENSGT00550000074802; -.
DR OMA; ILEQWHR; -.
DR Proteomes; UP000261440; Unplaced.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 3.
DR CDD; cd01460; vWA_midasin; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 6.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 7.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR PIRSF; PIRSF010340; Midasin; 2.
DR SMART; SM00382; AAA; 6.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340}.
FT DOMAIN 5191..5391
FT /note="VWFA"
FT /evidence="ECO:0000259|PROSITE:PS50234"
FT REGION 2976..2995
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3464..3502
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4418..4440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4594..4890
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4902..5099
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4604..4636
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4651..4671
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4672..4688
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4689..4768
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4769..4793
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4801..4872
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4935..4994
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5014..5031
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5032..5098
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5403 AA; 605960 MW; 0B3C1884112E7741 CRC64;
MEKLELSLTS LAAISRHVDK SHSELSKYLS KQVWNQQDRQ CILGCLAQLL LEKDYTLLIA
RHLRPLILDL LERNAERIKV DVRLNHDLHE RLCVAVSKLL SISPDAQTFA SRYFNSAPPV
FQRLFFTSEE SASIQYGPRR MKLRDLMGAT LRFLQSDCEK FRVLWDWSAC VSQLLTSDVM
VRGYTAQCLA LVSHMTDNQK TIFLRKVLSS EEILHLKMKS LEEAQQLEVE RALVLANQGS
VMWRLERGNK YTKGQVVSED LSQSVTAVCG VILPRLAPRQ EEQKSLQNLV LVDSTCRNLR
RLAMAVASQK PVLLEGPIGC GKTALVEYLA AVTGHVKAPD ILKVQLGDQT DSKMLLGMYR
CTDIPGKFVW QPGSLTQAVS KGHWILLEDI DHAPLDVISV LLPLMENKKL TIPGREDCIQ
VAPGFQFFAT RSGGSWHRPQ STHATLLDKY WSKLQICNMT RDELKQILLN RYPNLAAVVD
RLLDIYCQLT GDRHHVPVSS NLQTPGEEKP SLLEGRGLSL RDLLKWCERI CMNFDNSCSA
TAQNVFLEAL DCFTAMLSKA ESRLKLAEII GSKLNISREK AQHFCQMYQP GINVTELEVT
VGRAMLLRKQ SDAVRLEIEK QTFAATRPSS VLLEQLSICV TKGEPVLLVG ETGTGKTSTV
QYLAGLTGHK LRVVNMNQQS DTADLLGGYK PVDHKLLLLP LREAFEDLFS QTYSRKQNIT
FLGHVQTCFR GKRWQDLLKL MDHVCKSALT KELQDKPNAA LLQEQWEALA LKLSQTQEQI
RARESALVFA FVEGTLAQAV KKGEWVLLDE INLAAPETLE CLSGLLEGSA GSLVLLDRGD
TEPLVRHSDF RLFACMNPAT DVGKRNLPLG IRNRFTELYV EELESEADLR ILISDYLKGL
NLNRSTIHGI ISFYLAVRKE ANSRLADGTG HKPHYSLRTL CRALKYVAAN PCYNVQRSLY
EGFCLSFLTQ LDRSSHPLVE KLVYQYILGG NIKCLKQPIP EPRSRGFVQM EGYWLSQGDM
DPALDPSYIL TTSVQLNLRD LSRVVAAGIH PVLIQGETSV GKTSLVRWLA AATGNQCVRI
NNHEHTDIQE YIGCYSSDEH GKLVFKEGIL IDAMRKGYWI ILDELNLAPT DVLEALNRLL
DDNRELFVAE TQEVVKAHPR FMLFATQNPP GLYGGRKVLS RAFRNRFVEL HFDELPSEEL
ETILHQRCSL PPSYCTKLVK VMQDLQSLRR GSSVFAGKHG FITLRDLFRW AERYRLEEQT
DTSRDWLQHL ANDGYMLLAG RVRKPEEAST IQNILEKHFK RTVNPEVLFS EAQVTTQFSP
FVASIAGVPE EFGHVVWTQG MRRLAVLVGR ALRFGESVLL VGDTGCGKTT ICQLFAALAG
QKFFSVNCHL HMETSDFLGG LRPVRHAGTG HGDAEDGRLF EWRDGPLVLA MRQEGVFLMD
EISLADDSVL ERLNSVLETE KSLVLAEKGS GDDDDVELII AGKKFRLVAT MNPGGDFGKK
ELSPALRNRF TEIWCPQTNS RSDMMKIVQH NLRSGLFLDR HDHQGKDIAE LMLDFIDWLT
NQDFGRRCIL SVRDILSWVD FLNVVCERDE PRDENGFMAL AEVEEPEWDK RLDTVTAFIH
AACLIYVDSI GSGTTVSAAE NAVEARKACL TFLETKLRQI IELDKKMQEA LRVYDTTLLR
EPHWAEDFFG IEPFYIVSGP ESESRSLSDY ALGAGTTAMN AMRVLRALKL QRPILLEGSP
GVGKTSLVAA LAKASGNHLV RINLSEQTDV TDLFGTDLPV EGGKGGEFAW RDGPLLAALK
AGHWIVLDEL NLASQSVLEG LNACFDHRAE IYIPELSMRF QVQHEKTKIF GCQNPFTQGG
GRKGLPKSFL NRFTQVYVDQ LTSEDMQFIS NSIFPNINEQ IISKMVQFSN RLAHEVTVER
KWGQKGGPWE FNLRDLFRWC QLMQTDQSPA FYNPGQHVGL VYADRMRTDA DKAQVLSVFR
KVFTEDFEPY MGSRQFHITP LNLQVGSSVL QRSGGAPVAL DPPLSITHQA LRPLESIMKC
VKMGWMAILV GPTASGKSSL VHLLALLTGH RLRVMAMNSA MDTTELLGGF EQVDIIRPWQ
QVLESMDYVV AMVTRRGLMS LDGAVQDTEF LLSTWSTFRR WLRDEGLDTT EGALTFEALN
KLEVIIVLLH KLNTKLKVFT DMSKLQMDFT LLKERLAQME DGWSHGGFEW LDGMLVQALQ
AGDWLLMDNV NFCSPSVLDR LNALLEPGGS LTINERGVID GTTPSITPHP NFRLFLTMDP
AHGEISRAMR NRGVEIFIPG ENEGVCWDSL DLKTVLHTLG VTGDCVCDIL MSVHSEIKSA
IWDSPASSVS SLLHAAALLS GQLQRGVDLP SALLQACGEA YALCQRTAAN QQLAQEVIER
QLAVLDSEEW GSRLLCAGVW PDRVPSALQS TEDSCFSSVQ RDGQVLLYCL NTLSLHGKRG
RPLSLTDLQR AMQSGAVLEG LLFHGGGPGG GAEGGVNEAQ QLLPTAVRIL TERASHSDWV
LRTSWLSYLS KSYKHVPEAA MMQLEAGSRA LKAVFNSKLI GKGKYLTEHL QPHSTDDYRI
LVDMRWNKQY LDILANTCNF EDEERCMEFM EALYAVANRI IILMDREERA AIGSSTGNAS
PHNSALRLAT ILQLLQWRDR FWHVCNSLPA DACGVCVLSL HWQWVHKHLV LRLPQLLMGD
GDYEGQQELH TISTAIQNTL SSSVSVASAL KNIQKTLGKP LPFKLEAVAE CAARLRFLSR
ALDVKDLRLD DSTPSLWKRE VIRLRGVAMG IDSKEALLEG WGLVLQANLL LEPRNTGVLD
RLLTCVDQQQ GHLASCGLLD SLTEAADEEV MSDGAAPGRE VLLQLGRRAQ LWPVLEELAV
LNQMKVTADL LHLSVMQRGN KEAEAGLCRS LSNHLRFCMQ STPMNIRQLQ PLWFLLSTER
PVEELPLVWC ELLSETLAAL WSSSVTSDPE RWLKWSPLSP EEPKSSRSLL GPSDTGPGML
SRAVWSRCIL GVLSGSEVGG RWDPSGSSFL SSSRVTLGEW RERVGQLQDL SAVLWDNMAM
SELFDFRLTD CRLQGLVLCR QLHALSDLLS PGLQESYAES CESLIDGQDP VRATQEIQVA
LREGLPENLL PEGFLDTLVT CLTQFSHNRV QDAIQPARSG VFWVNMGLLQ IQVWAPQTIF
DPAVKRAYKL SYAQEELALL QDEWRGRSLS SQLLTGAELE ESSTTDAFQH PRIRYLWIRM
QQVKEQICEL SRKQACRPAD PQYGRLYQEL QHYLCSIGQP SAVGDLLSHL LKALQGSRPK
SRPGIQGLLK EEAVWQASQH RFAQRLAEEY PLYPDVVVPL RSAILQLQHG MRLLASQVSS
SLTALPGLPR LLSTLLAFPG PPLSSDLASA DFLCSRACID VLRGLGKLLP AAEAHRVVPE
PSVLLLSALL RLQSHSLTAG QLGTEARTLL RHVCQALVNE WDEQERRARE KEQREASLYR
TRSRLHGTGL TEEQQEEREF RRSFPTYHKD FADITAEPSL EQPIIIEDDD DDFEEAESSE
SSGFLLASCQ NTIVQVHQRL CLSYAQSLWY EPSPLTNQSK DHISALVSSY QITAPLIARF
YHLMDCEMDS QLTGSQLLLS SLLQNTVRGS GGPAGLILQS DGPYDFYREP NMSQARLCLP
AIEQLGQAVR QRLDDWPEHP ALVQITVVME RILAFSLSSP LAKFLNGLEI LLSKAQDWEN
NASRSVSLRN ELEPITQLII QWRKLELNCW SSSLDNALKR HAENSTKHWF SIYQLIERHL
QEKNSEGGVE ELSLSSVSST LQGFMEGSTL GEFHTRLTML LAFHCHLLLA PKQEGQGSLC
SLLWNLHKYY AQFSESVKSK MSQLRTPIEK ELKDFVKISK WNDVSFWSIK QSVEKTHRTL
FKFVKKFEAA LADPCAPCLV EQASSAGLDS VDTQLEETPL HRLHRALNSS LPGRGRDLQS
DGPEDIMSGV EPSSLQARLP FLTRRMRKMC VQLVKKMPLP ELSEDLDQFT AEIIANVQAL
QSLTVDRSME QERQKAEVKH ILLQKQRALA DLFKMLAQIG LSYRKGLTWG RTVDPEEPLC
LQPIEMSTAL SSVRTQDYTE TTLFSEMSSA WDGCQKYFYR SWARRNAMQA ALQTAAKELG
LGSVERCRGF SSHLFKLLLR QRRRLTKLTE HWVHLRKLID SIQDIRSHVT DSSKEEDSGL
PPQDSLHGWV VRAQALAQQC SAALQQFSWV LQCCPEGASA ALSQAGQGEA TLSHPSPLAP
HLQPQACLMR RGEAGWSEAV QRVEALHSES TQLKEKLNAL ELESTNGAIH TCHMEQCCSY
VYGLGSLAGR MVALEQLFVP EGTPVLNTQP SLTRGLHAVR GEVDTCVTEF TAWKSQLLSL
GQTSKRPATS FRAEFSEQVE RTINTVLCAV QSLVKRRERE SEGEGEQPAD SRRESDSEVA
EELLKQSHLS RLLEEDLSGD VSSLSVPDVV GVTEQLLERL RSHRDACSPQ HLGEIKEACR
FLVRLEPMLC VYSELVRWYV AVCMGAHRST GKLLSVLASI FTDLGQKGFC LPQELMGGGD
GEGATEFHDY EGGGIGEGEG VKDVSDKIEN EDQVEDTFQE GQEREEEPDQ RDIKEEDNAI
EMSEDFDGKM HDGEPREPGE DEEDSDEEQN EELDKKMGDL GDGPTDTLDE RMWGDEDNDD
DDLDGSDKEE ESGPGMDQGE SELVAKDDNT DAGESNKDKK NQDKDHPMDS EDQKEKVHEQ
IDEREFDENE VDPYHGKQEH KAEPEAMDLP EELNLDEDEQ GGENEEGEGE SENPYDIEDK
PMEVDEAEGE GEEEGSPEVM EDQQDGEGGE DEEQKGEEDT GEEQTEVDED SEKDERGGNE
GEEDEDEKEK GQEEGREEDK SIPADQGQKP KVCFVCLFEQ GSGAADASQA EGHDSKLMAR
MSSQRQKQKQ TQSFKRKPGR ADNERSLGDA NERVNKRLRT MESAERREQE QTSADALQES
DLYEHIRQGE EKYDAQTYDA ASTEQQRPAA MKQEDDDDDD DDEDDIAMDT DEEKELQAVE
APELKPEKMD SCKASKRGME EGEVESQKQA DKDEDEKPER KGSRDEEERK ERSRESTIHT
VPELLTETLK VPESAAAAMW QQYQTLTSPL SQQLCEQLRL ILEPTQASKL KGDYRTGKRL
NMRKVIPYIA SQFRKDKIWL RRTKPSKRDY QICLAVDDSS SMVDNHSKQL AFESVSVIVN
ALTLLEVGQV AVCSFGESVQ LLHPFHQQFN DQSGARILRL CQFQQKKTRI AQFLEASANM
FVAAKQQSPG SANTEMAQLL LIVSDGRGLF LEGKERVAAA VQAARSANVF VIFVVLDNPN
SRDSILDIKV PIFKGPGELP EIRSYMEEFP FPFYVILRDV NALPETLSDA LRQWFELVAA
TDQ
//
