ID A0A3B4CAM0_PYGNA Unreviewed; 587 AA.
AC A0A3B4CAM0;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE SubName: Full=CDK5 regulatory subunit associated protein 1 {ECO:0000313|Ensembl:ENSPNAP00000007931.1};
GN Name=CDK5RAP1 {ECO:0000313|Ensembl:ENSPNAP00000007931.1};
OS Pygocentrus nattereri (Red-bellied piranha).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Pygocentrus.
OX NCBI_TaxID=42514 {ECO:0000313|Ensembl:ENSPNAP00000007931.1, ECO:0000313|Proteomes:UP000261440};
RN [1] {ECO:0000313|Ensembl:ENSPNAP00000007931.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the methylthiotransferase family. MiaB
CC subfamily. {ECO:0000256|ARBA:ARBA00009815}.
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DR RefSeq; XP_017572036.1; XM_017716547.1.
DR AlphaFoldDB; A0A3B4CAM0; -.
DR Ensembl; ENSPNAT00000001008.1; ENSPNAP00000007931.1; ENSPNAG00000002871.1.
DR GeneID; 108438616; -.
DR CTD; 51654; -.
DR GeneTree; ENSGT00940000160361; -.
DR OMA; CEHFHIP; -.
DR OrthoDB; 1122at2759; -.
DR Proteomes; UP000261440; Unplaced.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035596; F:methylthiotransferase activity; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:InterPro.
DR Gene3D; 3.40.50.12160; Methylthiotransferase, N-terminal domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR HAMAP; MF_01864; tRNA_metthiotr_MiaB; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR005839; Methylthiotransferase.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR013848; Methylthiotransferase_N.
DR InterPro; IPR038135; Methylthiotransferase_N_sf.
DR InterPro; IPR006463; MiaB_methiolase.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR002792; TRAM_dom.
DR NCBIfam; TIGR00089; MiaB/RimO family radical SAM methylthiotransferase; 1.
DR PANTHER; PTHR43020; CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1; 1.
DR PANTHER; PTHR43020:SF2; MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF00919; UPF0004; 1.
DR SFLD; SFLDF00273; (dimethylallyl)adenosine_tRNA; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDF00413; CDK5RAP1; 1.
DR SFLD; SFLDG01061; methylthiotransferase; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51449; MTTASE_N; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
DR PROSITE; PS50926; TRAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 91..211
FT /note="MTTase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51449"
FT DOMAIN 233..488
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT DOMAIN 491..566
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT REGION 25..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..55
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 587 AA; 65994 MW; F042AE62CC6A7C5D CRC64;
MKLVSGLRLF AFSVQTYLPR RAPRYSSRCS ASSEDTGEKR EKRARRFESR LSSGPDLRDF
IRASSNKTPP EEPQTGREEP CLPRVSAANV RRVYFETYGC QMNVNDTEIA WSVLQKGGYL
RTSELSEADV VLLVTCSVRE KAEQTIWNRL KQLTALKKKR LKANSHLKIG ILGCMAERLK
TELLEKEKLV DVVAGPDAYR DLPRLLTLAH GGQRASNVLL SLEETYADVI PIHSTGHSAF
VSIMRGCDNM CSFCVVPFTR GRERSRPLSS ILEEVRMLSD QGVKEVTLLG QNVNSYRDIS
EAQFSISEAG LRLSRGFSTM YRAKQGGLRF AELLDRVSLI DPDMRIRFTS PHPKDFPDEV
LHLIQERKNI CKQIHLPAQS GSSRVLQTMR RGYTREAYLE LVENIRKIIP GVSLSSDFIT
GFCGETEEDH HQTLSLLREV GYNIGFLFAY SMRKKTHAYH RLQDDVPAEV KQRRLEELIS
VFREEAAKVN ATLIGSTQLV LVEGESKRSS QELCGRNDGN LKVIFPKKDL PSQTTVTSMV
PIEAGDYVLV KITSTNSQSL RGEAISHTSL SGGRRTEELL NDQAAAC
//
