ID   A0A3B4CDL6_PYGNA        Unreviewed;       815 AA.
AC   A0A3B4CDL6;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   24-JAN-2024, entry version 23.
DE   RecName: Full=Aminopeptidase {ECO:0000256|RuleBase:RU364040};
DE            EC=3.4.11.- {ECO:0000256|RuleBase:RU364040};
GN   Name=ANPEP {ECO:0000313|Ensembl:ENSPNAP00000010027.1};
OS   Pygocentrus nattereri (Red-bellied piranha).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC   Characoidei; Pygocentrus.
OX   NCBI_TaxID=42514 {ECO:0000313|Ensembl:ENSPNAP00000010027.1, ECO:0000313|Proteomes:UP000261440};
RN   [1] {ECO:0000313|Ensembl:ENSPNAP00000010027.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (SEP-2023) to UniProtKB.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|PIRSR:PIRSR634016-3,
CC         ECO:0000256|RuleBase:RU364040};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3,
CC       ECO:0000256|RuleBase:RU364040};
CC   -!- SIMILARITY: Belongs to the peptidase M1 family.
CC       {ECO:0000256|ARBA:ARBA00010136, ECO:0000256|RuleBase:RU364040}.
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DR   AlphaFoldDB; A0A3B4CDL6; -.
DR   Ensembl; ENSPNAT00000016601.1; ENSPNAP00000010027.1; ENSPNAG00000016254.1.
DR   GeneTree; ENSGT00940000154876; -.
DR   Proteomes; UP000261440; Unplaced.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd09601; M1_APN-Q_like; 1.
DR   Gene3D; 1.25.50.20; -; 1.
DR   Gene3D; 2.60.40.1910; -; 1.
DR   Gene3D; 1.10.390.10; Neutral Protease Domain 2; 2.
DR   Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR   InterPro; IPR045357; Aminopeptidase_N-like_N.
DR   InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR   InterPro; IPR024571; ERAP1-like_C_dom.
DR   InterPro; IPR034016; M1_APN-typ.
DR   InterPro; IPR001930; Peptidase_M1.
DR   InterPro; IPR014782; Peptidase_M1_dom.
DR   InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR   PANTHER; PTHR11533:SF271; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR   Pfam; PF11838; ERAP1_C; 1.
DR   Pfam; PF01433; Peptidase_M1; 2.
DR   Pfam; PF17900; Peptidase_M1_N; 1.
DR   PRINTS; PR00756; ALADIPTASE.
DR   SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR   SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|RuleBase:RU364040};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU364040};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR634016-3};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW   ECO:0000256|RuleBase:RU364040};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU364040};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT   DOMAIN          54..249
FT                   /note="Aminopeptidase N-like N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF17900"
FT   DOMAIN          284..360
FT                   /note="Peptidase M1 membrane alanine aminopeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF01433"
FT   DOMAIN          361..473
FT                   /note="Peptidase M1 membrane alanine aminopeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF01433"
FT   DOMAIN          626..768
FT                   /note="ERAP1-like C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF11838"
FT   ACT_SITE        356
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT   BINDING         355
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT   BINDING         359
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT   SITE            406
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ   SEQUENCE   815 AA;  92357 MW;  7CD49D46702CF661 CRC64;
     MVKCHFSTMC IICTVVSVVS VGLLVGIWTF QFLAYAETPA ALWENYRLPD NLLPDVYYVT
     LWPRLQQDDR GLYIFTGNSS VVFRCVKETD LILIHSNKLN LTTAGGHLVR LTAISDAPAP
     AISSTRMEVT TQYLVIQLNG KLKAGRSYEL FTEFIGELAD DLAGFYRSEY FEDGVRVVAT
     SQMHPTHARK TFPCFDEPAM KAVFHITVLH ERGTVALSNG IEITLNTTVD GTAVTITRFE
     PTQRMSSYLL ALVISDYTNV QSTESTLIRI WARRKAVTDG HCAYALNLTG RILQFFEDYY
     KFPYPLSKSQ IALPDFYFGA MENWGLVTYR ETNLFYDPLI SSNANKEKTA TIIAHELAHM
     KDLIILDNLH KAFAVDALAS SRPLSSKEED ITRPEQISEV FDTISYSKGA SVLRMLSDFL
     TEPVFLLGLK TYLNTFAYKN TVGSDLWDHL QRAVDTNGTV LLPMTVGEIM DRWVLQMGFP
     VVTINTVTGQ LSQKHFLLDP ESLVDAPSQF YEWIVPVNWM KSGVEQTPVW LLERSFHSEM
     KTVGEDWVLL NLNLTGHYRV NYNVENWERI LNQLNSNHQA KLIPVSLALN TTKFLAKETA
     YLPWQSALNN LDYFCLMFDQ TLVYESMQYN QVNAIRVACS TGVDGCQNLT AEWFTQWMQH
     PEQNIHPNLR STVYCSAVAA GGAKEWDFAW EMLQTASIAS EADKLMSALA CTKDTGLLEY
     LTFTLDPMKI RKQDATSVIV QIAGNVEGRS LAWNFITLNW RYIFTLLQFK EQNADVGFGS
     ASSALDQAIE KTRANMKWVS ENQREIRDWF RAEAA
//