ID   A0A3B4DA55_PYGNA        Unreviewed;       700 AA.
AC   A0A3B4DA55;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   27-MAR-2024, entry version 26.
DE   SubName: Full=Calpain-2 catalytic subunit-like {ECO:0000313|Ensembl:ENSPNAP00000020385.1};
OS   Pygocentrus nattereri (Red-bellied piranha).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC   Characoidei; Pygocentrus.
OX   NCBI_TaxID=42514 {ECO:0000313|Ensembl:ENSPNAP00000020385.1, ECO:0000313|Proteomes:UP000261440};
RN   [1] {ECO:0000313|Ensembl:ENSPNAP00000020385.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (SEP-2023) to UniProtKB.
CC   -!- SIMILARITY: Belongs to the peptidase C2 family.
CC       {ECO:0000256|ARBA:ARBA00007623}.
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DR   RefSeq; XP_017568686.1; XM_017713197.1.
DR   AlphaFoldDB; A0A3B4DA55; -.
DR   STRING; 42514.ENSPNAP00000020385; -.
DR   Ensembl; ENSPNAT00000030927.1; ENSPNAP00000020385.1; ENSPNAG00000027212.1.
DR   GeneID; 108436601; -.
DR   GeneTree; ENSGT00940000154784; -.
DR   OMA; DDSKEWD; -.
DR   OrthoDB; 142935at2759; -.
DR   Proteomes; UP000261440; Unplaced.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd00214; Calpain_III; 1.
DR   CDD; cd00044; CysPc; 1.
DR   Gene3D; 2.60.120.380; -; 1.
DR   Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR   Gene3D; 1.10.238.10; EF-hand; 1.
DR   InterPro; IPR033883; C2_III.
DR   InterPro; IPR022684; Calpain_cysteine_protease.
DR   InterPro; IPR022682; Calpain_domain_III.
DR   InterPro; IPR022683; Calpain_III.
DR   InterPro; IPR036213; Calpain_III_sf.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR000169; Pept_cys_AS.
DR   InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR   PANTHER; PTHR10183; CALPAIN; 1.
DR   PANTHER; PTHR10183:SF409; CALPAIN-8; 1.
DR   Pfam; PF01067; Calpain_III; 1.
DR   Pfam; PF00648; Peptidase_C2; 1.
DR   PRINTS; PR00704; CALPAIN.
DR   SMART; SM00720; calpain_III; 1.
DR   SMART; SM00230; CysPc; 1.
DR   SMART; SM00054; EFh; 3.
DR   SUPFAM; SSF49758; Calpain large subunit, middle domain (domain III); 1.
DR   SUPFAM; SSF54001; Cysteine proteinases; 1.
DR   SUPFAM; SSF47473; EF-hand; 1.
DR   PROSITE; PS50203; CALPAIN_CAT; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
DR   PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|ARBA:ARBA00022837};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00239}; Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW   ProRule:PRU00239};
KW   Thiol protease {ECO:0000256|ARBA:ARBA00022807, ECO:0000256|PROSITE-
KW   ProRule:PRU00239}.
FT   DOMAIN          45..344
FT                   /note="Calpain catalytic"
FT                   /evidence="ECO:0000259|PROSITE:PS50203"
FT   DOMAIN          572..607
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000259|PROSITE:PS50222"
FT   DOMAIN          609..637
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000259|PROSITE:PS50222"
FT   ACT_SITE        105
FT                   /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU00239"
FT   ACT_SITE        262
FT                   /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU00239"
FT   ACT_SITE        286
FT                   /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU00239"
SQ   SEQUENCE   700 AA;  78983 MW;  B776A584C1455CB7 CRC64;
     MSNIAHELSR RQDKEDGLGS NEKAIIFNNQ DFQSLQRECL ERRTLFCDPT FPADSSSLGY
     NQLGRYSSKT AGVEWKRPKE LCSNPLFISD GATRTDIRQG ALGDCWLLAA IASLTLDSEI
     LARVVPSAQS FTEDYAGIFH FQLWQYGEWL DVVIDDRLPT RNGELLFVHS AEGNEFWSAL
     LEKAYAKVNS SYEALSGGST TEGFEDFTGG ISESYELRKA PPTLFNIIKK ALSRGSLLGC
     SIDITGSYES EAITRQKLVK GHAYSVTGAE VVHVYGSPVE LVRLRNPWGQ VEWMGAWSDD
     SKEWDRVLPE EKAKLDYSAD DGEFWMAYSD FIQQYSKLEI CNLTPDTLSS DGVCRWSHHQ
     FEGTWRVGST AGGCRNNPAT FCSNPQFVIK LEEVDDDPHD GVDGCSFLVG LMQKDGRKER
     QFGRDLNTIG FAIYKVPDEY KGDNNVHLGP DVLLRQRAIA MSNTFINLRE VCGRFKLPPG
     EYVIIPSTFE PHLKGSFILR VFAEKQATAS PMESDIRADI KEEDVSEKDV DPHFKHLFKQ
     IAGNDSEISA VELKKILDRV VSQRTDLKTD GFSMETCRHI ISLMDRDGNG TLGLLEFHTL
     WMKLQKYLEI FKNRDMDNSG TMSSHEIREA LIEAGFQLNT SVLQVIISRF ADLESAIDFD
     CFVGCLIRLE MLFQMFTKFD KKNTGKIELD IMQWLCLALS
//