ID A0A3B4EAH6_PYGNA Unreviewed; 817 AA.
AC A0A3B4EAH6;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Trimethylguanosine synthase {ECO:0000256|ARBA:ARBA00018517};
OS Pygocentrus nattereri (Red-bellied piranha).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Pygocentrus.
OX NCBI_TaxID=42514 {ECO:0000313|Ensembl:ENSPNAP00000033547.1, ECO:0000313|Proteomes:UP000261440};
RN [1] {ECO:0000313|Ensembl:ENSPNAP00000033547.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-
CC ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end
CC (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-(ribonucleoside) in
CC mRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:67624,
CC Rhea:RHEA-COMP:17171, Rhea:RHEA-COMP:17315, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:167623,
CC ChEBI:CHEBI:172880; Evidence={ECO:0000256|ARBA:ARBA00024488};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67625;
CC Evidence={ECO:0000256|ARBA:ARBA00024488};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside
CC in mRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-
CC triphosphoguanosine)-ribonucleoside in mRNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:67620, Rhea:RHEA-COMP:17167, Rhea:RHEA-
CC COMP:17315, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:156461, ChEBI:CHEBI:172880;
CC Evidence={ECO:0000256|ARBA:ARBA00024618};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67621;
CC Evidence={ECO:0000256|ARBA:ARBA00024618};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC Trimethylguanosine synthase family. {ECO:0000256|ARBA:ARBA00025783}.
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DR RefSeq; XP_017561479.1; XM_017705990.1.
DR AlphaFoldDB; A0A3B4EAH6; -.
DR STRING; 42514.ENSPNAP00000033547; -.
DR Ensembl; ENSPNAT00000024545.1; ENSPNAP00000033547.1; ENSPNAG00000022249.1.
DR GeneID; 108432263; -.
DR CTD; 96764; -.
DR GeneTree; ENSGT00390000018056; -.
DR OMA; GWETYWA; -.
DR OrthoDB; 5473515at2759; -.
DR Proteomes; UP000261440; Unplaced.
DR GO; GO:0008168; F:methyltransferase activity; IEA:InterPro.
DR GO; GO:0009452; P:7-methylguanosine RNA capping; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR019012; RNA_cap_Gua-N2-MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR14741; S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED; 1.
DR PANTHER; PTHR14741:SF32; TRIMETHYLGUANOSINE SYNTHASE; 1.
DR Pfam; PF09445; Methyltransf_15; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
FT REGION 59..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 105..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..387
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 492..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..157
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..266
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 320..352
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 353..385
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 504..520
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 569..585
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 817 AA; 91903 MW; 4211251D4CC953C4 CRC64;
MVNRGKWQTV PIADIYFHHG VLDEMTIYCL CTRAFVNDRE LYRSDLKSLQ ALTDSVLQDN
VEDLEDEEPS NSEDEEDVEM DEETILMASM GLPVAFGSSS AQRKAHKWTG YTPHRGSKKA
NAGHQEAFSE QEDPQQDPEL EPVKNEQQEE KEKVVPVVLE DTPADPESTI SETPWLNYWQ
LHGETLLWGS WVEKHPEFSS GTPLAPWDCA EWKEAWQEHA SQGYLYYWEQ FDYWAAQGWT
TDESYSAAAH DDGQCREDEL VEEKNKPSSN WSENMADLNA VDIAERFDSL NLLTDENGSN
DGQQAFDCNC ADEPCDGGDQ KRPSSSSSVC TKEGNKQASS SSVTNKNGQP VRSGSNKKDE
SDDDEPPDRK QAKIKRSHEL DVEELAQVPS EEAWETLGLK RSPQLKFTSV LKFREAPGQR
EGSQSGARWR PTGRKATCKI NKHIFFTEDG QTAVPKISQT LQKVQSFLQR IQTENDSEKE
AASVLGSTTL QARVSTVDGQ GTLRPQENEE ECREPQPDSG SMESEEPQPL TQPEPEPETL
AAPDSDPLQD SDSDPRGELQ PLDIPDYLLP DAPKDKQGDT EASTKKKKKK KAKRWRREVD
VPPEIAAEPE LAKYWAQRYR LFSRFDEGIK LDHEGWFSVT PEKIAEHIAQ RVREVYDTQL
IVDAFCGVGG NAIQFALTGK RVIAIDIDPV RLELAHHNAE VYGVAQRIDF IQGDFLQLAP
RLRADVVFLS PPWGGPEYLS AEVFNIKTMM SPDGFEIFRL AKMISENIVY FLPRNADMEQ
IASLAGPGGK VEVEQNFLNN KLKTITAYFG SLVKADS
//
