ID A0A3B4EBC3_PYGNA Unreviewed; 503 AA.
AC A0A3B4EBC3;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=Fumarate hydratase, mitochondrial {ECO:0000256|ARBA:ARBA00013409};
DE EC=4.2.1.2 {ECO:0000256|ARBA:ARBA00012921};
OS Pygocentrus nattereri (Red-bellied piranha).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Pygocentrus.
OX NCBI_TaxID=42514 {ECO:0000313|Ensembl:ENSPNAP00000033135.1, ECO:0000313|Proteomes:UP000261440};
RN [1] {ECO:0000313|Ensembl:ENSPNAP00000033135.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the hydration of fumarate to L-malate in the
CC tricarboxylic acid (TCA) cycle to facilitate a transition step in the
CC production of energy in the form of NADH.
CC {ECO:0000256|ARBA:ARBA00003146}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00024594};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12461;
CC Evidence={ECO:0000256|ARBA:ARBA00024594};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00024453};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:12462;
CC Evidence={ECO:0000256|ARBA:ARBA00024453};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate
CC from fumarate: step 1/1. {ECO:0000256|ARBA:ARBA00004859}.
CC -!- SIMILARITY: Belongs to the class-II fumarase/aspartase family. Fumarase
CC subfamily. {ECO:0000256|ARBA:ARBA00009084}.
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DR RefSeq; XP_017573380.1; XM_017717891.1.
DR AlphaFoldDB; A0A3B4EBC3; -.
DR STRING; 42514.ENSPNAP00000033135; -.
DR Ensembl; ENSPNAT00000037758.1; ENSPNAP00000033135.1; ENSPNAG00000021593.1.
DR GeneID; 108439454; -.
DR CTD; 2271; -.
DR GeneTree; ENSGT00950000183122; -.
DR OMA; AKWRAQT; -.
DR OrthoDB; 1341425at2759; -.
DR UniPathway; UPA00223; UER01007.
DR Proteomes; UP000261440; Unplaced.
DR GO; GO:0045239; C:tricarboxylic acid cycle enzyme complex; IEA:InterPro.
DR GO; GO:0004333; F:fumarate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0006106; P:fumarate metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01362; Fumarase_classII; 1.
DR Gene3D; 1.10.40.30; Fumarase/aspartase (C-terminal domain); 1.
DR Gene3D; 1.20.200.10; Fumarase/aspartase (Central domain); 1.
DR Gene3D; 1.10.275.10; Fumarase/aspartase (N-terminal domain); 1.
DR HAMAP; MF_00743; FumaraseC; 1.
DR InterPro; IPR005677; Fum_hydII.
DR InterPro; IPR024083; Fumarase/histidase_N.
DR InterPro; IPR018951; Fumarase_C_C.
DR InterPro; IPR020557; Fumarate_lyase_CS.
DR InterPro; IPR000362; Fumarate_lyase_fam.
DR InterPro; IPR022761; Fumarate_lyase_N.
DR InterPro; IPR008948; L-Aspartase-like.
DR NCBIfam; TIGR00979; fumC_II; 1.
DR PANTHER; PTHR11444; ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASE; 1.
DR PANTHER; PTHR11444:SF1; FUMARATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF10415; FumaraseC_C; 1.
DR Pfam; PF00206; Lyase_1; 1.
DR PRINTS; PR00145; ARGSUCLYASE.
DR PRINTS; PR00149; FUMRATELYASE.
DR SUPFAM; SSF48557; L-aspartase-like; 1.
DR PROSITE; PS00163; FUMARATE_LYASES; 1.
PE 3: Inferred from homology;
FT DOMAIN 51..382
FT /note="Fumarate lyase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00206"
FT DOMAIN 448..500
FT /note="Fumarase C C-terminal"
FT /evidence="ECO:0000259|Pfam:PF10415"
SQ SEQUENCE 503 AA; 54189 MW; 147B1A99D5CB8330 CRC64;
MYRTVRNLQR FNSSLSALQC CTAIRTLGPT PHTPLRMASS ESYRIERDTF GELKVPSDKY
YGAQTVRSTM NFKIGGVTER MPIQVIRAFG ILKRAAAEVN KDYGLDPKIA GAIVQAAEEV
TAGKLDDHFP LVVWQTGSGT QTNMNVNEVI SNRAIEILGG KLGSKDPVHP NDHVNKSQSS
NDTFPTAMHI AAAKEVHEVL LPGLQELHDA LAAKAEQFKD IIKIGRTHTQ DAVPLSLGQE
FGGYLQQVKY SIERVKAAMP RVYELAAGGT AVGTGLNTRI GFAEKVADRV SALTGLPFVT
AVNKFEALAA HDALVELSGA LNTVAVSMMK IANDIRFLGS GPRSGLGELI LPENEPGSSI
MPGKVNPTQC EAMTMVAAQV MGNHVAVTVG GSNGHFELNV FKPMIIKNVL NSAQLLGDAS
VSFTKNCVVG IEANTERINK LMNESLMLVT ALNPHIGYDK AASIAKTAHK EGSTLKATAI
KLGYLTEEQF DQWVRPQDML GPK
//
