ID A0A3B5R9F3_XIPMA Unreviewed; 312 AA.
AC A0A3B5R9F3;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=General transcription factor IIH subunit 3 {ECO:0000256|RuleBase:RU368090};
DE AltName: Full=General transcription factor IIH polypeptide 3 {ECO:0000256|RuleBase:RU368090};
OS Xiphophorus maculatus (Southern platyfish) (Platypoecilus maculatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Cyprinodontiformes; Poeciliidae; Poeciliinae;
OC Xiphophorus.
OX NCBI_TaxID=8083 {ECO:0000313|Ensembl:ENSXMAP00000040252.1, ECO:0000313|Proteomes:UP000002852};
RN [1] {ECO:0000313|Proteomes:UP000002852}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JP 163 A {ECO:0000313|Proteomes:UP000002852};
RA Walter R., Schartl M., Warren W.;
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000002852}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JP 163 A {ECO:0000313|Proteomes:UP000002852};
RX PubMed=23542700; DOI=10.1038/ng.2604;
RA Schartl M., Walter R.B., Shen Y., Garcia T., Catchen J., Amores A.,
RA Braasch I., Chalopin D., Volff J.N., Lesch K.P., Bisazza A., Minx P.,
RA Hillier L., Wilson R.K., Fuerstenberg S., Boore J., Searle S.,
RA Postlethwait J.H., Warren W.C.;
RT "The genome of the platyfish, Xiphophorus maculatus, provides insights into
RT evolutionary adaptation and several complex traits.";
RL Nat. Genet. 45:567-572(2013).
RN [3] {ECO:0000313|Ensembl:ENSXMAP00000040252.1}
RP IDENTIFICATION.
RC STRAIN=JP 163 A {ECO:0000313|Ensembl:ENSXMAP00000040252.1};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000256|RuleBase:RU368090}.
CC -!- SUBUNIT: Part of a TFIID-containing RNA polymerase II pre-initiation
CC complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1,
CC GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2,
CC ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10,
CC TAF11, TAF12 and TAF13. Component of the 7-subunit TFIIH core complex
CC composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and
CC GTF2H5, which is active in NER. The core complex associates with the 3-
CC subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H,
CC CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in
CC transcription. Interacts with RARA; the interaction requires prior
CC phosphorylation of RARA on 'Ser-369' which then enhances interaction of
CC RARA with CDK7. {ECO:0000256|RuleBase:RU368090}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU368090}.
CC -!- SIMILARITY: Belongs to the TFB4 family. {ECO:0000256|ARBA:ARBA00005273,
CC ECO:0000256|RuleBase:RU368090}.
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DR AlphaFoldDB; A0A3B5R9F3; -.
DR STRING; 8083.ENSXMAP00000040252; -.
DR Ensembl; ENSXMAT00000027465.1; ENSXMAP00000040252.1; ENSXMAG00000017500.2.
DR GeneTree; ENSGT00390000013143; -.
DR InParanoid; A0A3B5R9F3; -.
DR OMA; CFCHRKV; -.
DR Proteomes; UP000002852; Unassembled WGS sequence.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR004600; TFIIH_Tfb4/GTF2H3.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12831:SF0; GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3; 1.
DR PANTHER; PTHR12831; TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED; 1.
DR Pfam; PF03850; Tfb4; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU368090};
KW DNA repair {ECO:0000256|RuleBase:RU368090};
KW Metal-binding {ECO:0000256|RuleBase:RU368090};
KW Nucleus {ECO:0000256|RuleBase:RU368090};
KW Reference proteome {ECO:0000313|Proteomes:UP000002852};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU368090};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|RuleBase:RU368090}; Zinc {ECO:0000256|RuleBase:RU368090};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|RuleBase:RU368090}.
SQ SEQUENCE 312 AA; 34595 MW; E2E909A5E14F6AA3 CRC64;
MPLIGKHYVF DSCNAGSFLL HWVDICHVFF AEDELNLLVI VVDVNPIWWG QQGQREPEFT
LCKCLDAVMV MGNSHMAMTR TNRLAVIASH CQDSHFLYPS KTWKSGDSGG DEISSSRDGK
YELLAVANNL IAEEIRNIMA KTEVTGNSTD TLLAGSLAKA LCCILSVIKA ADDCALQYMN
FMNVIFAAQK QNILIDACVL DSDSGLLQQA CDITGGLYLK IPQKVALAQY LLWVFLPDSE
QRSQLVLPPP AHVDYRAACF CHRNLIEIGY VCSVCLSIFC NFSPICTTCE TAFKIQLPQM
VKPKKKKLKQ AA
//
