ID A0A3B5ZQE9_WHEAT Unreviewed; 503 AA.
AC A0A3B5ZQE9;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=CFC21_010578 {ECO:0000313|EMBL:KAF6993732.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS1D02G090100.1};
RN [1] {ECO:0000313|EMBL:KAF6993732.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF6993732.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS1D02G090100.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS1D02G090100.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS1D02G090100.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF6993732.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF6993732.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; CM022213; KAF6993732.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B5ZQE9; -.
DR STRING; 4565.A0A3B5ZQE9; -.
DR PaxDb; 4565-Traes_1DS_21CA6101F-2; -.
DR EnsemblPlants; TraesCS1D02G090100.1; TraesCS1D02G090100.1; TraesCS1D02G090100.
DR Gramene; TraesCAD_scaffold_005268_01G000200.1; TraesCAD_scaffold_005268_01G000200.1; TraesCAD_scaffold_005268_01G000200.
DR Gramene; TraesCLE_scaffold_006670_01G000200.1; TraesCLE_scaffold_006670_01G000200.1; TraesCLE_scaffold_006670_01G000200.
DR Gramene; TraesCS1D02G090100.1; TraesCS1D02G090100.1; TraesCS1D02G090100.
DR Gramene; TraesCS1D03G0210100.1; TraesCS1D03G0210100.1.CDS; TraesCS1D03G0210100.
DR Gramene; TraesPAR_scaffold_023277_01G000100.1; TraesPAR_scaffold_023277_01G000100.1; TraesPAR_scaffold_023277_01G000100.
DR Gramene; TraesROB_scaffold_003011_01G000200.1; TraesROB_scaffold_003011_01G000200.1; TraesROB_scaffold_003011_01G000200.
DR Gramene; TraesWEE_scaffold_018229_01G000200.1; TraesWEE_scaffold_018229_01G000200.1; TraesWEE_scaffold_018229_01G000200.
DR OMA; IQCGSAH; -.
DR Proteomes; UP000019116; Chromosome 1D.
DR Proteomes; UP000815260; Chromosome 1D.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF22; ENDOGLUCANASE 18-RELATED; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..16
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 17..503
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5040524925"
FT DOMAIN 35..485
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 411
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
SQ SEQUENCE 503 AA; 54038 MW; 124397F381D8D8FA CRC64;
MAVKALVVAM MLVVHCFDVG LGAGGDGLCL GSPDYGDALA KAILFFEGQR SGRLPANQRA
RWRGNSALAD GRPENVNLTG GYYDAGDNVK FGFPMAFTVT LLSWSAIEYR GDVAAAGQLG
NLRAAIHWGA DFLLRAHTSP TTLYTQVGDG NADHGCWERP EDMDTPRTLY KITGDSPGSE
AAGEAAAALA AAYLVLKEDG DKAFASRLLA ASKSLFELAN NYRGSFQSSC PFYCSYSGFQ
DELLWASAWL YRATKDGKYL NFLRDNQGSS NPVNEFSWDN KQVGAQMLAT QEYLKGRREL
ARYKTNLDSF VCALMPDSGN TQIRTTRGAL LFTRDSVNMQ YTTTAALVLS IYSKTLQSWG
VSDGVRCSAA SFSPDQIASF AASQVDYILG SNPMGMSYMV GFGGAFPRRI HHRAASIPSI
KVLSRRVPCN EGFSSWLPTS DPNPNVHVGA IVGGPDENDQ FGDNRGDSAH SEPATYINAA
FVGACAAAMG QSQKPASPTL ASY
//
