ID A0A3B6B2H0_WHEAT Unreviewed; 220 AA.
AC A0A3B6B2H0;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=6,7-dimethyl-8-ribityllumazine synthase {ECO:0000256|ARBA:ARBA00012664, ECO:0000256|RuleBase:RU003795};
DE Short=DMRL synthase {ECO:0000256|RuleBase:RU003795};
DE EC=2.5.1.78 {ECO:0000256|ARBA:ARBA00012664, ECO:0000256|RuleBase:RU003795};
GN ORFNames=CFC21_017884 {ECO:0000313|EMBL:KAF7002386.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS2A02G352600.1};
RN [1] {ECO:0000313|EMBL:KAF7002386.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7002386.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS2A02G352600.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS2A02G352600.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS2A02G352600.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7002386.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7002386.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by
CC condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-
CC butanone 4-phosphate. This is the penultimate step in the biosynthesis
CC of riboflavin. {ECO:0000256|RuleBase:RU003795}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-
CC ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2
CC H2O + phosphate; Xref=Rhea:RHEA:26152, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15934, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:58201, ChEBI:CHEBI:58830; EC=2.5.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001697,
CC ECO:0000256|RuleBase:RU003795};
CC -!- PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; riboflavin
CC from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-
CC ribitylamino)uracil: step 1/2. {ECO:0000256|ARBA:ARBA00004917,
CC ECO:0000256|RuleBase:RU003795}.
CC -!- SIMILARITY: Belongs to the DMRL synthase family.
CC {ECO:0000256|ARBA:ARBA00007424, ECO:0000256|RuleBase:RU003795}.
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DR EMBL; CM022214; KAF7002386.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6B2H0; -.
DR SMR; A0A3B6B2H0; -.
DR STRING; 4565.A0A3B6B2H0; -.
DR EnsemblPlants; TraesCS2A02G352600.1; TraesCS2A02G352600.1; TraesCS2A02G352600.
DR Gramene; TraesCAD_scaffold_061663_01G000200.1; TraesCAD_scaffold_061663_01G000200.1; TraesCAD_scaffold_061663_01G000200.
DR Gramene; TraesCLE_scaffold_060674_01G000200.1; TraesCLE_scaffold_060674_01G000200.1; TraesCLE_scaffold_060674_01G000200.
DR Gramene; TraesCS2A02G352600.1; TraesCS2A02G352600.1; TraesCS2A02G352600.
DR Gramene; TraesCS2A03G0861500.1; TraesCS2A03G0861500.1.CDS; TraesCS2A03G0861500.
DR Gramene; TraesKAR2A01G0373260.1; cds.TraesKAR2A01G0373260.1; TraesKAR2A01G0373260.
DR Gramene; TraesPAR_scaffold_056838_01G000200.1; TraesPAR_scaffold_056838_01G000200.1; TraesPAR_scaffold_056838_01G000200.
DR Gramene; TraesRN2A0100840000.1; TraesRN2A0100840000.1; TraesRN2A0100840000.
DR Gramene; TraesROB_scaffold_016743_01G000200.1; TraesROB_scaffold_016743_01G000200.1; TraesROB_scaffold_016743_01G000200.
DR Gramene; TraesWEE_scaffold_126749_01G000200.1; TraesWEE_scaffold_126749_01G000200.1; TraesWEE_scaffold_126749_01G000200.
DR OMA; HCREEYQ; -.
DR OrthoDB; 276496at2759; -.
DR UniPathway; UPA00275; UER00404.
DR Proteomes; UP000019116; Chromosome 2A.
DR Proteomes; UP000815260; Chromosome 2A.
DR GO; GO:0009349; C:riboflavin synthase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0000906; F:6,7-dimethyl-8-ribityllumazine synthase activity; IBA:GO_Central.
DR GO; GO:0009231; P:riboflavin biosynthetic process; IBA:GO_Central.
DR CDD; cd09209; Lumazine_synthase-I; 1.
DR Gene3D; 3.40.50.960; Lumazine/riboflavin synthase; 1.
DR HAMAP; MF_00178; Lumazine_synth; 1.
DR InterPro; IPR034964; LS.
DR InterPro; IPR002180; LS/RS.
DR InterPro; IPR036467; LS/RS_sf.
DR NCBIfam; TIGR00114; lumazine-synth; 1.
DR PANTHER; PTHR21058:SF0; 6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE; 1.
DR PANTHER; PTHR21058; 6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE; 1.
DR Pfam; PF00885; DMRL_synthase; 1.
DR SUPFAM; SSF52121; Lumazine synthase; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Riboflavin biosynthesis {ECO:0000256|ARBA:ARBA00022619,
KW ECO:0000256|RuleBase:RU003795};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003795}.
SQ SEQUENCE 220 AA; 22418 MW; 7726F79905C898B1 CRC64;
MAASPATSSS AARATSFARL SNAPLRAPRA GAVSFPSPNS ARPAALVADA RASRLPVVAA
AAAGHQRLMG SLTNTEGLRF AVVVARFNEI VTNLLLQGAL EAFERYSIKA ENVTVVSVPG
SFEIPVAAQK LGKSGKYDAI LCIGAVIRGD TTHYDAVANS AASGVLNAGL SAGVPCVFGV
LTCDDMDQAL NRAGGKAGNK GAETAITAIE MASLFRHHLA
//
