ID A0A3B6DG84_WHEAT Unreviewed; 1059 AA.
AC A0A3B6DG84;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=CFC21_030661 {ECO:0000313|EMBL:KAF7017182.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS2D02G354400.2};
RN [1] {ECO:0000313|EMBL:KAF7017182.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7017182.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS2D02G354400.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS2D02G354400.2};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS2D02G354400.2}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7017182.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7017182.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; CM022216; KAF7017182.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6DG84; -.
DR SMR; A0A3B6DG84; -.
DR STRING; 4565.A0A3B6DG84; -.
DR EnsemblPlants; TraesCS2D02G354400.2; TraesCS2D02G354400.2; TraesCS2D02G354400.
DR Gramene; TraesCS2D02G354400.2; TraesCS2D02G354400.2; TraesCS2D02G354400.
DR Gramene; TraesCS2D03G0813300.2; TraesCS2D03G0813300.2.CDS; TraesCS2D03G0813300.
DR OMA; CEDIYRS; -.
DR Proteomes; UP000019116; Chromosome 2D.
DR Proteomes; UP000815260; Chromosome 2D.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IBA:GO_Central.
DR GO; GO:0032543; P:mitochondrial translation; IBA:GO_Central.
DR GO; GO:0009791; P:post-embryonic development; IEA:UniProt.
DR GO; GO:0048608; P:reproductive structure development; IEA:UniProt.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116}.
FT DOMAIN 91..733
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 779..942
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1059 AA; 117939 MW; 1F7D13C162D7FE44 CRC64;
MDAASCCRVF STQRCPFPLR RLGAAPFSTG LSELFDPSSS VSKRRSRGPV MAAKKAADGT
KQKDGKYKHT VDLPKTTFDL RANSVMREPE LQKLWEENQV LKRVSERNTG ATFTLHDGPP
YANGDLHMGH ALNKVLKDII NRYKLLQNHK VSFIPGWDCH GLPIELKVLK SMDKATLDAL
TPIKLRQKAA KFAKATVNAQ MNSFKRIGIW ADWDNPYLTL SPEYEAAQLE VFGQMVMRGY
IYRGRKPVHW SPSSRTALAE AELEYSENHV SKSIYAAFKI TSPSNSGLLD EFLPNVCLAI
WTTTPWTIPA NAAVAVNPEL SYAVVELQSL LESESTSGGK QRKLGSILSS GNEKPFIIVA
SDLVSVLESK WGVKLVIRKS FPGSVLEYCR NLHPVNGNEC SVVIGGDYIT TESGTGLVHT
APGHGQEDYL TGLKYGLPIV SPVDDEGNFT AEAGQFSGLS VLGAGNAAVV KYLDEHVSLI
LEEPYKHKYP YDWRSKEPTI FRATEQWFAS VDGFRDAALD AIKRVTWVPS QGENRIVNMI
SGRSDWCISR QRTWGVPIPV FYHVDTQEPL ITEKTIEHIK GIVSEKGSDA WWYMPTEELL
PEKYRDKASE YRKGTDTMDV WFDSGSSWAA VSAKRDGLNF PADVYLEGSD QHRGWFQSSL
LTSIATTGKA PYSSVITHGF VLDEEGFKMS KSVGNVVDPE KVIVGGKNSK EEPPYGADVL
RLWVSSVDYT GDVLIGSQIL RQMSDMYRKL RGTMRFLLAN LHDWKPENSV PYSDLPKIDQ
YALFQLENVV ASMKDGYDNY QFYKIYQTLQ RFAIVGLSNF YFDVAKDRLY VGGRVSCTRK
SCQTVLAAHL LYLVRAIAPI MPHLAEDIWQ NLPFQHTLED GSVAKFAFDL KWPDKNEEWC
SVQKDDVDFL GVILELRSEV NKILESARTG KLIGASLDAK VYLHAENPDT VSKLKELASA
TNDADALHRL FITSQVEILP SLSEETKLGV SYAGKFSDPR TGEIWIGATR ADGVKCERCW
VYTKDVGSFL DHPTLCSRCH GVIDLQPQAS PATAAAAVA
//
