ID A0A3B6DJX9_WHEAT Unreviewed; 1906 AA.
AC A0A3B6DJX9;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 13 {ECO:0000256|ARBA:ARBA00019618, ECO:0000256|RuleBase:RU364134};
GN ORFNames=CFC21_032054 {ECO:0000313|EMBL:KAF7018810.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS2D02G494900.1};
RN [1] {ECO:0000313|EMBL:KAF7018810.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7018810.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS2D02G494900.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS2D02G494900.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS2D02G494900.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7018810.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7018810.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. {ECO:0000256|RuleBase:RU364134}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000256|RuleBase:RU364134}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU364134}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC {ECO:0000256|ARBA:ARBA00009354, ECO:0000256|RuleBase:RU364134}.
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DR EMBL; CM022216; KAF7018810.1; -; Genomic_DNA.
DR EnsemblPlants; TraesCS2D02G494900.1; TraesCS2D02G494900.1; TraesCS2D02G494900.
DR Gramene; TraesCS2D02G494900.1; TraesCS2D02G494900.1; TraesCS2D02G494900.
DR Gramene; TraesCS2D03G1103900.2; TraesCS2D03G1103900.2.CDS; TraesCS2D03G1103900.
DR Gramene; TraesKAR2D01G0430280.1; cds.TraesKAR2D01G0430280.1; TraesKAR2D01G0430280.
DR OMA; LLVTDWC; -.
DR Proteomes; UP000019116; Chromosome 2D.
DR Proteomes; UP000815260; Chromosome 2D.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR InterPro; IPR009401; Med13_C.
DR InterPro; IPR021643; Mediator_Med13_N.
DR InterPro; IPR041285; MID_MedPIWI.
DR PANTHER; PTHR48249; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13; 1.
DR PANTHER; PTHR48249:SF3; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13; 1.
DR Pfam; PF06333; Med13_C; 1.
DR Pfam; PF11597; Med13_N; 1.
DR Pfam; PF18296; MID_MedPIWI; 1.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|RuleBase:RU364134};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU364134};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Repressor {ECO:0000256|ARBA:ARBA00022491, ECO:0000256|RuleBase:RU364134};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU364134};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|RuleBase:RU364134}.
FT DOMAIN 2..243
FT /note="Mediator complex subunit Med13 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11597"
FT DOMAIN 1099..1332
FT /note="MID"
FT /evidence="ECO:0000259|Pfam:PF18296"
FT DOMAIN 1480..1882
FT /note="Mediator complex subunit Med13 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06333"
FT REGION 451..473
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 706..738
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1027..1053
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1619..1646
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 720..738
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1027..1042
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1906 AA; 207986 MW; 425EBA6FE48C05AC CRC64;
MWTNIFKIGE LQTVSWFQFL PVEPDYSATS DRSSKAEQKD ALNSTVLSAY LRLQSEGLLS
TWTNSFVGPW DPSQGEHNPD EKIKLWLFLS GRHSSVPEMT QPAVAKLRVV SSGLWVAPGN
SEEVAAALCQ ALRNSLERAL RGLSYARFGD VFTKYNPPTR NQNSFRRAQP TVEFVFAATE
EAIFVHVIIS ARYMRNLSSD DIEKVLTHSP RSVGEGLPVI VAPSGMLGRL VGCCPSDLAI
QVYSSKLSAP NLPGFTQPTI CQLRGQSYYV EVALGFPPAS TDKISESENN QIKEEFDSVK
EPHVGDYGQK KLESADGLPV LERTFIYPPE AVMVPMVHQA FVRFSSKRMW SQDWMGSSSW
EAWPFWNFSP SSYFRNSSFF GSSRGLGVNS NFLRLRRQRN NNSNGMASSI SSVSSTSNGS
EHAVAAKGGD LLADADSTAC HQSDLPLNND IAGSKMVSKR SRSEITEDSS HAGKEVRENM
QGTNGQGGCS WGWGEEGVVM DINILLSEFG DFSDFFQGDE LDFGEPPGTA ESHALVSPAS
EYGDMPFIDS PSVAMDIPEQ RLSPVGFTSM EAFNHQTMSP IQDVASKVQE PLKEIASPAG
SQSLVLSSSR SDFLTRAEAT LTFAPEYAAV EISSCETPAT LFTNPYLPGS KKRGSCGFSS
RVYSYDVTQS SKVESAGDKS EKSDKLTPAN LSRDVGRSSL YTLVQGRKNE SEKSLNNADE
QSCKGETSRP VSGETSFSSS LTIQKKSDSM LNVGYFLLSM KTALATEIEC ITFQAAMCRI
RHTLVSLRTK ASAELKSALS SAMQTESSSN SGLVPKYEMK RKESIPARLS SDVDHDMYDR
SQLENVGVWR SVVVPKGAKP LDSLSAKTFT GTSPSVQRQP IVELLSAMAL LVQQSTSFVD
IALDMDDGDG SFFWLSLDEQ RRRGFSCDPS MVHAGCGGLL GTCHSKDCAG VDLVDPLSAE
VSESSMIGLL QSDIKSALKT AFANMDGPLS VIDWCRGRSN IAESAAMGDA YSFHYTTGDI
RDTSNSIPIG GDAMSPPQSS NDRGTSEEHH KGYHRVRPTI AVLPSPSLLV GYQDDWLKTS
ANCLKLWEKA PLEPYASAKP VTYYALCPDI DMLTSAATDF FMQLGTIYEV CKLGTHLPQH
SGGQIEHSPG KYLPSGLVLV ECPDQLKTRG SHSVSISSVT EYFQALSKSW SVKSFVSSLA
RIIKDIKLTV NISTNQKESS NIPCTVIYVV CPFPEPSAVL QALVESSVAL GSILSSERER
KSFLYTQVAK ALDGSASADE ASASNVVMLS GFSIPKLVLQ IVTVETLLRL HKPNELAAFK
DIAFTVYNKA RRIPRFVSTS DMFQSPTYMS RPQSTMMHTA SPGPTLWKEC LVPRMSGQTL
SRETEFDASM RSVSWDNSWQ PGRAVGLPDP SKIPELCAQD DRKYAFEPLF ILAEPGTADY
SDMMESSRFG ADASSSRAYS SISGGTDSGA SPPLEGSEND SATSLHCCYG WTEDWRWLVC
IWTDSRGELL DSLIFPFGGI SSRQDTKVLQ SLFIQILQQG CQIMSSSPEA SNTRSRDVII
TRIGGFLELE IQEWQKAIYS FGGNEVKKWP VQLRRSIPDG IPSNSNGPTL QQQDMGLIQD
RNMPSSPSTL YSPHSKSSFT KGQSGNKKQI LVEQTGMDSS RGSLHLVRSI SLVAVSQDSS
LHLACQADLM ATRPTSGEGN QSSTGSSSYL DGFAPVKSIG SMSASYLLVP SPSMRYLSPA
TLQLPMCLTS ESPPLAHLLH SKGTATPLAM GYVVSKAVPP VRKNAAQLTK EDRHSVLSVS
IIDYYGGSTP TVQEKMSRGV GGSTMSKQAR NITHETSARD YEMDMHNVLE AVAAELHALS
WMTVSPVYPE RRSALPFHCD MVLRLRRLLH YADRHLSQLT EKGDLA
//
