ID A0A3B6ECX8_WHEAT Unreviewed; 340 AA.
AC A0A3B6ECX8;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=CFC21_101095 {ECO:0000313|EMBL:KAF7099466.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS3A02G032200.1};
RN [1] {ECO:0000313|EMBL:KAF7099466.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7099466.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS3A02G032200.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS3A02G032200.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS3A02G032200.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7099466.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7099466.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the SINA (Seven in absentia) family.
CC {ECO:0000256|ARBA:ARBA00009119}.
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DR EMBL; CM022230; KAF7099466.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6ECX8; -.
DR STRING; 4565.A0A3B6ECX8; -.
DR PaxDb; 4565-Traes_3AS_45C7AACB0-2; -.
DR EnsemblPlants; TraesCS3A02G032200.1; TraesCS3A02G032200.1; TraesCS3A02G032200.
DR Gramene; TraesCAD_scaffold_157288_01G000100.1; TraesCAD_scaffold_157288_01G000100.1; TraesCAD_scaffold_157288_01G000100.
DR Gramene; TraesCLE_scaffold_130346_01G000100.1; TraesCLE_scaffold_130346_01G000100.1; TraesCLE_scaffold_130346_01G000100.
DR Gramene; TraesCS3A02G032200.1; TraesCS3A02G032200.1; TraesCS3A02G032200.
DR Gramene; TraesCS3A03G0064900.1; TraesCS3A03G0064900.1.CDS; TraesCS3A03G0064900.
DR Gramene; TraesPAR_scaffold_014403_01G000100.1; TraesPAR_scaffold_014403_01G000100.1; TraesPAR_scaffold_014403_01G000100.
DR Gramene; TraesROB_scaffold_139053_01G000100.1; TraesROB_scaffold_139053_01G000100.1; TraesROB_scaffold_139053_01G000100.
DR Gramene; TraesWEE_scaffold_140607_01G000100.1; TraesWEE_scaffold_140607_01G000100.1; TraesWEE_scaffold_140607_01G000100.
DR OMA; TICFEHL; -.
DR OrthoDB; 395309at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000019116; Chromosome 3A.
DR Proteomes; UP000815260; Chromosome 7B.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd16571; RING-HC_SIAHs; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 2.
DR InterPro; IPR049548; Sina-like_RING.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR013010; Znf_SIAH.
DR PANTHER; PTHR10315; E3 UBIQUITIN PROTEIN LIGASE SIAH; 1.
DR PANTHER; PTHR10315:SF81; RING-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF21362; Sina_RING; 1.
DR Pfam; PF21361; Sina_ZnF; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR SUPFAM; SSF49599; TRAF domain-like; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
DR PROSITE; PS51081; ZF_SIAH; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PROSITE-ProRule:PRU00455};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU00455};
KW Zinc-finger {ECO:0000256|PROSITE-ProRule:PRU00455}.
FT DOMAIN 32..69
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT DOMAIN 86..144
FT /note="SIAH-type"
FT /evidence="ECO:0000259|PROSITE:PS51081"
SQ SEQUENCE 340 AA; 38084 MW; 41FA51781251D843 CRC64;
MDGSNSNKRR DEAPREGDRW MDVRILKETL DCTVCFEHFR TEIYQCSVGH FICSSCRDKI
LDKKCPTCSI KTSFKRCFGM EHVVQSVAFP CSNAKYGCRE GHAYYQKEGH EQVCPYGPCF
CPASDCSFTG LTHELLDHLT IYHMSPCTDL PYHGTVALRL EPGIHVLAPR NKGNNHFFML
NMEPERLGYA ISVVCVQPNA TEPKFTCSMS YDCSGTGYCG SASCQIKSST LSDGLPTDYD
LIPPKRKISG DRKFIMLRIT IHQASSVSKS HVKGKGLTST PLTLFPRRSD DDGDIVCRSC
IEGKCPTSSQ KLLFPDYSSD DDDDVPSRFE RALKRVSLLD
//
