ID A0A3B6EK67_WHEAT Unreviewed; 444 AA.
AC A0A3B6EK67;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN ORFNames=CFC21_036017 {ECO:0000313|EMBL:KAF7023516.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS3A02G274200.1};
RN [1] {ECO:0000313|EMBL:KAF7023516.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7023516.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS3A02G274200.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS3A02G274200.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS3A02G274200.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7023516.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7023516.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001678};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM022217; KAF7023516.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6EK67; -.
DR SMR; A0A3B6EK67; -.
DR STRING; 4565.A0A3B6EK67; -.
DR EnsemblPlants; TraesCS3A02G274200.1; TraesCS3A02G274200.1; TraesCS3A02G274200.
DR Gramene; TraesCS3A02G274200.1; TraesCS3A02G274200.1; TraesCS3A02G274200.
DR Gramene; TraesCS3A03G0693900.1; TraesCS3A03G0693900.1.CDS; TraesCS3A03G0693900.
DR OMA; YHDGFSI; -.
DR OrthoDB; 2717493at2759; -.
DR Proteomes; UP000019116; Chromosome 3A.
DR Proteomes; UP000815260; Chromosome 3A.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451:SF45; MANNAN ENDO-1,4-BETA-MANNOSIDASE 2; 1.
DR PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|RuleBase:RU361153};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 7..26
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 66..364
FT /note="Glycoside hydrolase family 5"
FT /evidence="ECO:0000259|Pfam:PF00150"
SQ SEQUENCE 444 AA; 49882 MW; 614C4BFB03B36A86 CRC64;
MAACNGLFVY HILGLASLLA VFYFSLLGEV DLRFPGLLPS PGASRSHDAS LPFVERRGAQ
LFLEGRPFYI NGWNSYWLMD QAVEPASRHR VSDMFRAATG MGLTVCRTWA FNDGAYNALQ
LSPGHFDERV FKALDMVVVE ARRHGVRLIL SLANNLEAYG GKTQYVRWAW DEGVGLTASN
DSFFFDPAIR DYFKVYLKAL LTRTNHLTGV QYKDDPTILA WELMNEPRCI TDPSGNTLQR
WIDEMAGYVK SIDRRHLLTV GTEGFYGPTS PPEKLSVNPG HWFNNYGLDF IRNSKISDID
FASVHLYPDT WLLDANLEEK LKFVTKWVSS HFEDGDTELG GKPVVLTEFG LSHLVKGFEQ
SQRDAFYKSV YDIVHESAKR GGAGAGAIVW QLAAEDMEEY HDGFAIVPSE TPSMQKLLTE
QSCRLAALRH GEQEAKRILK AVCG
//
