ID A0A3B6EMZ2_WHEAT Unreviewed; 777 AA.
AC A0A3B6EMZ2;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=Subtilisin-like protease {ECO:0008006|Google:ProtNLM};
GN ORFNames=CFC21_037527 {ECO:0000313|EMBL:KAF7025331.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS3A02G420500.1.cds1};
RN [1] {ECO:0000313|EMBL:KAF7025331.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7025331.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS3A02G420500.1.cds1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS3A02G420500.1.cds1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS3A02G420500.1.cds1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7025331.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7025331.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase S8 family.
CC {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240,
CC ECO:0000256|RuleBase:RU003355}.
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DR EMBL; CM022217; KAF7025331.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6EMZ2; -.
DR SMR; A0A3B6EMZ2; -.
DR STRING; 4565.A0A3B6EMZ2; -.
DR EnsemblPlants; TraesCS3A02G420500.1; TraesCS3A02G420500.1.cds1; TraesCS3A02G420500.
DR Gramene; TraesCAD_scaffold_052207_01G000100.1; TraesCAD_scaffold_052207_01G000100.1; TraesCAD_scaffold_052207_01G000100.
DR Gramene; TraesCLE_scaffold_040444_01G000400.1; TraesCLE_scaffold_040444_01G000400.1; TraesCLE_scaffold_040444_01G000400.
DR Gramene; TraesCS3A02G420500.1; TraesCS3A02G420500.1.cds1; TraesCS3A02G420500.
DR Gramene; TraesCS3A03G0975800.1; TraesCS3A03G0975800.1.CDS1; TraesCS3A03G0975800.
DR Gramene; TraesKAR3A01G0407710.1; cds.TraesKAR3A01G0407710.1; TraesKAR3A01G0407710.
DR Gramene; TraesPAR_scaffold_113168_01G000100.1; TraesPAR_scaffold_113168_01G000100.1; TraesPAR_scaffold_113168_01G000100.
DR Gramene; TraesROB_scaffold_069948_01G000100.1; TraesROB_scaffold_069948_01G000100.1; TraesROB_scaffold_069948_01G000100.
DR Gramene; TraesWEE_scaffold_028704_01G000100.1; TraesWEE_scaffold_028704_01G000100.1; TraesWEE_scaffold_028704_01G000100.
DR OMA; DFPETAC; -.
DR OrthoDB; 11910at2759; -.
DR Proteomes; UP000019116; Chromosome 3A.
DR Proteomes; UP000815260; Chromosome 3A.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0016485; P:protein processing; IBA:GO_Central.
DR CDD; cd02120; PA_subtilisin_like; 1.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 2.60.40.2310; -; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 3.30.70.80; Peptidase S8 propeptide/proteinase inhibitor I9; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PROPROTEIN CONVERTASE SUBTILISIN/KEXIN; 1.
DR PANTHER; PTHR10795:SF542; SUBTILISIN-LIKE PROTEASE SBT1.5; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..777
FT /note="Subtilisin-like protease"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039990624"
FT DOMAIN 27..106
FT /note="Inhibitor I9"
FT /evidence="ECO:0000259|Pfam:PF05922"
FT DOMAIN 134..597
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 374..460
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT DOMAIN 664..770
FT /note="Subtilisin-like protease fibronectin type-III"
FT /evidence="ECO:0000259|Pfam:PF17766"
FT ACT_SITE 143
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 216
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 548
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 777 AA; 79705 MW; 9BE42D1F6D56C930 CRC64;
MPRHLLLPLL LVAAVGASGG DAGGERTYIV RVDADAKPSA FPTHAHWYES AVLAASGGGG
GWPEGGPLIH TYSSALHGFS ARMSPSAAAA LAGARGVAAV LPERVRCLDT TRSPRFLGML
SSPPSAILAD SDFGSDLVIA VIDTGISPAH RSFRDRGLGP VPPRWRGACA SGPGFPPGSC
NRKLVGARFF SAGYEATSGR MNETAEVRSP LDNDGHGTHT ASIAAGRYVF PASTLGYARG
VASGMAPKAR LAAYKVCWAG GCFDSDILAA FDAAVADGVD VVSLSVGGAV VPYYLDAIAI
GAFGATEAGI VVSASAGNGG PGGLSVTNVA PWMTTVGAGS MDRAFPANVR LGNGQVLDGV
SVYGGPVLQS GKMYELVYAG ATSYSASTCL DGSLDQAAVR GKIVVCDRGV NSRAAKGDVV
HRAGAAGMVL ANGAFDGEGL VADCHVLPAT AVGAAAGEKL RKYIASSTPQ KPATGTIVFE
GTHLGVHPAP VVAAFSARGP NPQSPETLKP DLIAPGLNIL AAWPSGVGPA GIPSDGRRTE
FNILSGTSMA CPHVSGLAAL LKAAHPTWSP AAIKSALMTT AYTRDNSNGT MLDESTGKVA
DVFDFGAGHV DPMRAMDPGL VYDIAPADYV SFLCNLNYTG QNIRAITRRQ ADCRGARRAG
HAGNLNYPSL SVTFVADGAR AKMRTHFIRT VTNVGGGRSA YRATVRSPEG CNVTVRPDRL
AFRRDGQKLS FTVHVEAAAR ARMEPGSSLV RSGALTWGDG RHAVVSPIVV TLQAPVQ
//
