ID A0A3B6GSD8_WHEAT Unreviewed; 410 AA.
AC A0A3B6GSD8;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN ORFNames=CFC21_047567 {ECO:0000313|EMBL:KAF7037107.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS3D02G241300.2};
RN [1] {ECO:0000313|EMBL:KAF7037107.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7037107.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS3D02G241300.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS3D02G241300.2};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS3D02G241300.2}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7037107.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7037107.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001678};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
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DR EMBL; CM022219; KAF7037107.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6GSD8; -.
DR PaxDb; 4565-Traes_3B_4F1F03BB2-1; -.
DR EnsemblPlants; TraesCS3D02G241300.2; TraesCS3D02G241300.2; TraesCS3D02G241300.
DR Gramene; TraesCS3D02G241300.2; TraesCS3D02G241300.2; TraesCS3D02G241300.
DR Gramene; TraesCS3D03G0567700.2; TraesCS3D03G0567700.2.CDS; TraesCS3D03G0567700.
DR Gramene; TraesRN3D0100595300.2; TraesRN3D0100595300.2; TraesRN3D0100595300.
DR Proteomes; UP000019116; Chromosome 3D.
DR Proteomes; UP000815260; Chromosome 3D.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR PANTHER; PTHR31451:SF60; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|RuleBase:RU361153};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..410
FT /note="mannan endo-1,4-beta-mannosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039991029"
FT DOMAIN 69..322
FT /note="Glycoside hydrolase family 5"
FT /evidence="ECO:0000259|Pfam:PF00150"
SQ SEQUENCE 410 AA; 44130 MW; 35B3207392EBF636 CRC64;
MGLHHGAPLA LSVLVLLCVH GGREAEAAGG GFVRAQGTRF VINGSPYYAN GFNAYWLMTM
AADPAQRGKV TSALSQAAAR GLSVARTWAF SDGGSNALQY APGSYNENTF KGLDFVLSEA
RKNGIKVILS LVNNYDTFGG KKQYVEWARG RGQAVGSEDD FFTNSVVKGF YKNHVKNDPT
ILAWELMNEP RCQSDLSGRT IQSWITEMAA HVKSIDGNHM LEAGLEGFYG ASSSRAASVN
PAGRQVGTDF IANNQVPGID FATVHSYPDQ WLSSSDDQAQ RSFLGRWLDA HIADAQGTLR
KPLLIAEFGK SQRDPGFSSA QRDAQFGMVY AKIYESARRG GPTVGGLFWQ LMADGMDSYG
DGYQVIFAEA PASTTGVITS ESRKLKMLGK AFARAERAKR DASGKGTGGN
//
