ID A0A3B6H2M2_WHEAT Unreviewed; 633 AA.
AC A0A3B6H2M2;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN ORFNames=CFC21_048984 {ECO:0000313|EMBL:KAF7038887.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS3D02G379100.1};
RN [1] {ECO:0000313|EMBL:KAF7038887.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7038887.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS3D02G379100.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS3D02G379100.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS3D02G379100.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7038887.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7038887.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR EMBL; CM022219; KAF7038887.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6H2M2; -.
DR EnsemblPlants; TraesCS3D02G379100.1; TraesCS3D02G379100.1; TraesCS3D02G379100.
DR Gramene; TraesCAD_scaffold_018478_01G000300.1; TraesCAD_scaffold_018478_01G000300.1; TraesCAD_scaffold_018478_01G000300.
DR Gramene; TraesCLE_scaffold_025361_01G000300.1; TraesCLE_scaffold_025361_01G000300.1; TraesCLE_scaffold_025361_01G000300.
DR Gramene; TraesCS3D02G379100.1; TraesCS3D02G379100.1; TraesCS3D02G379100.
DR Gramene; TraesCS3D03G0836900.1; TraesCS3D03G0836900.1.CDS; TraesCS3D03G0836900.
DR Gramene; TraesPAR_scaffold_024995_01G000200.1; TraesPAR_scaffold_024995_01G000200.1; TraesPAR_scaffold_024995_01G000200.
DR Gramene; TraesROB_scaffold_052399_01G000300.1; TraesROB_scaffold_052399_01G000300.1; TraesROB_scaffold_052399_01G000300.
DR Gramene; TraesWEE_scaffold_124190_01G000200.1; TraesWEE_scaffold_124190_01G000200.1; TraesWEE_scaffold_124190_01G000200.
DR Proteomes; UP000019116; Chromosome 3D.
DR Proteomes; UP000815260; Chromosome 3D.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF179; BETA-GALACTOSIDASE 6; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..633
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039991313"
FT DOMAIN 54..378
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 402..456
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
SQ SEQUENCE 633 AA; 71841 MW; 49234E9E231870BF CRC64;
MSMAAAAAAI MAVLLAALMA RASSAAAAGR VWADEEKGAH NGTARRQVTY DGRSLMLDGA
RRMLFSGDIH YPRSTPEMWP RLIESAREGG LDVIQTYVFW NVHEPIQGQY NFEGRYDLVK
FIREIHAQGL YVSLRVGPFV EAEWKYGGLP FWLRGVPNIT FRCNNEPYKV QVYTQVKTDV
MYEVRVVHMQ KFVAKIVNMM KDENLYYPQG GPIIISQIEN EYKLVQAAFH SRGPPYVRWA
AAMAVNLQTG IPWMMCKQDD APDPIINTCN GLICGETFHG PNSPNKPALW TENWTSRYPL
YGHDPRFRSP ADIAFAVALF IARKKGSFVS YYMYHGGTNF GRFASSYVTT SYYDGAPLDE
YGLIWQSTWS HLRELHAAVK QSEEPLLSGA YSNYSFGEQQ EGHVFKTESN CVAFLVNFDK
HKTSNIQFGE ASFQLAPKSI SILSSCRRVV FETAKINAQH GLRTAQVVQY LNNVDSWKVF
KEPIPLAINN STHIGNRLFE HLSTTKDETD YLWYLTRYDY RSNGDTQLVL NVESQAHVLH
AYINNDYIGS VHGSHDGPRN IVLKTPIMLR KGQNSISLLS VMVGSPDSGA YMERRIFGVR
KVSIQQGHQK SHSLNNELWK HQVNPGWLIW RNE
//
