ID A0A3B6IKF4_WHEAT Unreviewed; 300 AA.
AC A0A3B6IKF4;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=16S rRNA (uracil(1498)-N(3))-methyltransferase {ECO:0000256|ARBA:ARBA00012328};
DE EC=2.1.1.193 {ECO:0000256|ARBA:ARBA00012328};
GN ORFNames=CFC21_056552 {ECO:0000313|EMBL:KAF7047659.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS4B02G059600.1};
RN [1] {ECO:0000313|EMBL:KAF7047659.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7047659.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS4B02G059600.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS4B02G059600.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS4B02G059600.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7047659.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7047659.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC ribosomal subunit. {ECO:0000256|ARBA:ARBA00025699}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC Evidence={ECO:0000256|ARBA:ARBA00000792};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC {ECO:0000256|ARBA:ARBA00005528}.
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DR EMBL; CM022221; KAF7047659.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6IKF4; -.
DR SMR; A0A3B6IKF4; -.
DR STRING; 4565.A0A3B6IKF4; -.
DR EnsemblPlants; TraesCS4B02G059600.1; TraesCS4B02G059600.1; TraesCS4B02G059600.
DR Gramene; TraesCAD_scaffold_003025_01G000500.1; TraesCAD_scaffold_003025_01G000500.1; TraesCAD_scaffold_003025_01G000500.
DR Gramene; TraesCLE_scaffold_000909_01G000100.1; TraesCLE_scaffold_000909_01G000100.1; TraesCLE_scaffold_000909_01G000100.
DR Gramene; TraesCS4B02G059600.1; TraesCS4B02G059600.1; TraesCS4B02G059600.
DR Gramene; TraesCS4B03G0129500.1; TraesCS4B03G0129500.1.CDS; TraesCS4B03G0129500.
DR Gramene; TraesPAR_scaffold_002199_01G000100.1; TraesPAR_scaffold_002199_01G000100.1; TraesPAR_scaffold_002199_01G000100.
DR Gramene; TraesRN4B0100134500.1; TraesRN4B0100134500.1; TraesRN4B0100134500.
DR Gramene; TraesROB_scaffold_000861_01G000900.1; TraesROB_scaffold_000861_01G000900.1; TraesROB_scaffold_000861_01G000900.
DR Gramene; TraesWEE_scaffold_001970_01G000100.1; TraesWEE_scaffold_001970_01G000100.1; TraesWEE_scaffold_001970_01G000100.
DR Proteomes; UP000019116; Chromosome 4B.
DR Proteomes; UP000815260; Chromosome 4B.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070042; F:rRNA (uridine-N3-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR Gene3D; 3.40.1280.10; -; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR006700; RsmE.
DR InterPro; IPR046886; RsmE_MTase_dom.
DR InterPro; IPR046887; RsmE_PUA-like.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR NCBIfam; TIGR00046; RsmE family RNA methyltransferase; 1.
DR PANTHER; PTHR30027:SF3; 16S RRNA (URACIL(1498)-N(3))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR30027; RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE E; 1.
DR Pfam; PF04452; Methyltrans_RNA; 1.
DR Pfam; PF20260; PUA_4; 1.
DR SUPFAM; SSF75217; alpha/beta knot; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 62..108
FT /note="Ribosomal RNA small subunit methyltransferase E PUA-
FT like"
FT /evidence="ECO:0000259|Pfam:PF20260"
FT DOMAIN 123..273
FT /note="Ribosomal RNA small subunit methyltransferase E
FT methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04452"
SQ SEQUENCE 300 AA; 32754 MW; B8F22240E1DC102B CRC64;
MPPAATFSSF SALMRRPPPP ARRLLVAAAR AHSTAGASRA RGGLPRFHSP SLPSSKGEVV
RIQGDEFWHM TRVLRLGIND RVELFDGAGG LVEGSIQKVD KNGSDVELVE NARLIAPQGI
QWHVFAAFGT LKGGRADWLI EKCTELGASS VTPLLTERCH TIAENRVDRL QRLVLAAVKQ
CQRVHDMSLK PPIQIHDLPL AVSTHYPFFV TFARNTATNQ MLMHWLLGIF CYQVSQSKLA
FLASAEAPPV LSVLPKCSSE EESGLLIIGP EGGNVVTLAC FLSIYLSKCF NESDTWSVLA
//
