ID A0A3B6KD15_WHEAT Unreviewed; 313 AA.
AC A0A3B6KD15;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=rRNA N-glycosylase {ECO:0000256|RuleBase:RU004915};
DE EC=3.2.2.22 {ECO:0000256|RuleBase:RU004915};
GN ORFNames=CFC21_064110 {ECO:0000313|EMBL:KAF7056731.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5A02G037200.1};
RN [1] {ECO:0000313|EMBL:KAF7056731.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7056731.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5A02G037200.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5A02G037200.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5A02G037200.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7056731.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7056731.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC adenosine on the 28S rRNA.; EC=3.2.2.22;
CC Evidence={ECO:0000256|RuleBase:RU004915};
CC -!- SIMILARITY: Belongs to the ribosome-inactivating protein family.
CC {ECO:0000256|RuleBase:RU004915}.
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DR EMBL; CM022223; KAF7056731.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6KD15; -.
DR SMR; A0A3B6KD15; -.
DR STRING; 4565.A0A3B6KD15; -.
DR EnsemblPlants; TraesCS5A02G037200.1; TraesCS5A02G037200.1; TraesCS5A02G037200.
DR Gramene; TraesCS5A02G037200.1; TraesCS5A02G037200.1; TraesCS5A02G037200.
DR Gramene; TraesCS5A03G0095000.1; TraesCS5A03G0095000.1.CDS; TraesCS5A03G0095000.
DR Proteomes; UP000019116; Chromosome 5A.
DR Proteomes; UP000815260; Chromosome 5A.
DR GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.420.10; Ricin (A subunit), domain 1; 1.
DR InterPro; IPR036041; Ribosome-inact_prot_sf.
DR InterPro; IPR001574; Ribosome_inactivat_prot.
DR InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR PANTHER; PTHR33453; -; 1.
DR PANTHER; PTHR33453:SF11; RRNA N-GLYCOSYLASE; 1.
DR Pfam; PF00161; RIP; 1.
DR SUPFAM; SSF56371; Ribosome inactivating proteins (RIP); 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|RuleBase:RU004915};
KW Plant defense {ECO:0000256|RuleBase:RU004915};
KW Protein synthesis inhibitor {ECO:0000256|RuleBase:RU004915};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Signal {ECO:0000256|SAM:SignalP}; Toxin {ECO:0000256|RuleBase:RU004915}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..313
FT /note="rRNA N-glycosylase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039992471"
SQ SEQUENCE 313 AA; 33652 MW; 9012DBED5FC66D4F CRC64;
MGSGNAIKVL QAAVLLLVVC GGSASVPATP SGNHPAIPAS DAGHGAAFEG QAAGVANPQI
LAVDFQVGAD SYEASFKSLH VALQANTVPV EGHYILPKET PKPEMWINLT LKGEAARPPA
LLLRSDNVYL LGFIAADGTA FCTKGKKKIF PVDCTELPFE DNYGGLTNRR EKEDLNGLLE
AVSLGKSEAQ AAADALARFK HGTTSPEVAR KSLLAFTLMI PEAQRFHSIG NKMKTDWIKS
SHLTEDQVKL IFVWRVLSAL WCRGDNPPDG EWSKAKDKLK ALNIKSRADV SRALDMVKNE
GRCSDLRKEK PPA
//
