ID A0A3B6KEE9_WHEAT Unreviewed; 676 AA.
AC A0A3B6KEE9;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=glutamine--fructose-6-phosphate transaminase (isomerizing) {ECO:0000256|ARBA:ARBA00012916};
DE EC=2.6.1.16 {ECO:0000256|ARBA:ARBA00012916};
GN ORFNames=CFC21_065168 {ECO:0000313|EMBL:KAF7058033.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5A02G139500.1};
RN [1] {ECO:0000313|EMBL:KAF7058033.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7058033.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5A02G139500.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5A02G139500.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5A02G139500.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7058033.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7058033.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-
CC phosphate + L-glutamate; Xref=Rhea:RHEA:13237, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:58725, ChEBI:CHEBI:61527; EC=2.6.1.16;
CC Evidence={ECO:0000256|ARBA:ARBA00001031};
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DR EMBL; CM022223; KAF7058033.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6KEE9; -.
DR SMR; A0A3B6KEE9; -.
DR STRING; 4565.A0A3B6KEE9; -.
DR EnsemblPlants; TraesCS5A02G139500.1; TraesCS5A02G139500.1; TraesCS5A02G139500.
DR Gramene; TraesCAD_scaffold_145820_01G000100.1; TraesCAD_scaffold_145820_01G000100.1; TraesCAD_scaffold_145820_01G000100.
DR Gramene; TraesCLE_scaffold_144135_01G000200.1; TraesCLE_scaffold_144135_01G000200.1; TraesCLE_scaffold_144135_01G000200.
DR Gramene; TraesCS5A02G139500.1; TraesCS5A02G139500.1; TraesCS5A02G139500.
DR Gramene; TraesCS5A03G0390300.1; TraesCS5A03G0390300.1.CDS; TraesCS5A03G0390300.
DR Gramene; TraesPAR_scaffold_064851_01G000200.1; TraesPAR_scaffold_064851_01G000200.1; TraesPAR_scaffold_064851_01G000200.
DR Gramene; TraesROB_scaffold_053467_01G000200.1; TraesROB_scaffold_053467_01G000200.1; TraesROB_scaffold_053467_01G000200.
DR Gramene; TraesWEE_scaffold_010065_01G000200.1; TraesWEE_scaffold_010065_01G000200.1; TraesWEE_scaffold_010065_01G000200.
DR OMA; ASEYRYA; -.
DR OrthoDB; 1705390at2759; -.
DR Proteomes; UP000019116; Chromosome 5A.
DR Proteomes; UP000815260; Chromosome 5A.
DR GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR GO; GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IBA:GO_Central.
DR GO; GO:0006002; P:fructose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR GO; GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IBA:GO_Central.
DR CDD; cd00714; GFAT; 1.
DR CDD; cd05008; SIS_GlmS_GlmD_1; 1.
DR CDD; cd05009; SIS_GlmS_GlmD_2; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR005855; GFAT.
DR InterPro; IPR047084; GFAT_N.
DR InterPro; IPR035466; GlmS/AgaS_SIS.
DR InterPro; IPR035490; GlmS/FrlB_SIS.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR001347; SIS_dom.
DR InterPro; IPR046348; SIS_dom_sf.
DR NCBIfam; TIGR01135; glmS; 1.
DR PANTHER; PTHR10937; GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING; 1.
DR PANTHER; PTHR10937:SF0; GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING); 1.
DR Pfam; PF13522; GATase_6; 1.
DR Pfam; PF01380; SIS; 2.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
DR SUPFAM; SSF53697; SIS domain; 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
DR PROSITE; PS51464; SIS; 2.
PE 4: Predicted;
KW Aminotransferase {ECO:0000256|ARBA:ARBA00022576};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..280
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000259|PROSITE:PS51278"
FT DOMAIN 352..492
FT /note="SIS"
FT /evidence="ECO:0000259|PROSITE:PS51464"
FT DOMAIN 523..666
FT /note="SIS"
FT /evidence="ECO:0000259|PROSITE:PS51464"
SQ SEQUENCE 676 AA; 73684 MW; 57EC10F0F226F588 CRC64;
MCGIFAYLNY NVSRERRYVL EVLLNGLRRL EYRGYDSSGI AVDADLPAPP APGSAPAPYA
GAPPLVYRQE GKIENLVRSV YAEVDEKDVN LDAAFNVHAG IAHTRWATHG VPAPRNSHPQ
SSGAGDEFLV VHNGIITNYE VLKETLTRHG FTFESDTDTE VIPKLAKFVF DKAHDGEGDV
TFSQVVNEVM RQLEGAYALI FKSPHYPNEL IACKRGSTLI LGVNELSGQK SGKSFNDVKA
LTANGKPKEL FFSSDLCAIV EHTKNYLAIE DNEIVHIKDG SVSILKFDHD KEKPASVQRA
LSVLEMEVEQ IKKGNYDHFM QKEIHEQPHS LTTTMRGRLK DGAVLLGGLK EHLKTIRRSR
RVVFIGCGTS YNAALAARTF VEELTGIPVT MEVASDLLDR QGPIYREDTA VFVSQSGETA
DTLLALDYAL ENGALCVGIT NTVGSTLSRR THCGIHINAG CEIGVASTKA YTSQIVVMAM
MALAIGSDQI STQARREAII SGLFSLPSNA SQVLKLDSEM KELASSLIDS ESLLVFGRGY
NYATALEGAL KVKEVALMHS EGMFAGEMKH GPLALVDENL PIIVIATRDA CFSKQQSVIQ
QLLSRKGRLI IMCSKGDASA VNPGGSCRVI EVPKVADCLQ PVINIIPLQL LAYHLTVLRG
FDVDQPRNLA KSVTTQ
//
