ID A0A3B6KKN1_WHEAT Unreviewed; 481 AA.
AC A0A3B6KKN1;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KAF7060174.1, ECO:0000313|EnsemblPlants:TraesCS5A02G321900.1};
GN ORFNames=CFC21_066981 {ECO:0000313|EMBL:KAF7060174.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5A02G321900.1};
RN [1] {ECO:0000313|EMBL:KAF7060174.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7060174.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5A02G321900.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5A02G321900.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5A02G321900.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7060174.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7060174.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC Evidence={ECO:0000256|ARBA:ARBA00029340};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004167}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the long-chain O-
CC acyltransferase family. {ECO:0000256|ARBA:ARBA00024360}.
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DR EMBL; CM022223; KAF7060174.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6KKN1; -.
DR PaxDb; 4565-Traes_5DL_6AB44B491-1; -.
DR EnsemblPlants; TraesCS5A02G321900.1; TraesCS5A02G321900.1; TraesCS5A02G321900.
DR Gramene; TraesCLE_scaffold_011685_01G000100.1; TraesCLE_scaffold_011685_01G000100.1; TraesCLE_scaffold_011685_01G000100.
DR Gramene; TraesCS5A02G321900.1; TraesCS5A02G321900.1; TraesCS5A02G321900.
DR Gramene; TraesCS5A03G0782100.2; TraesCS5A03G0782100.2.CDS; TraesCS5A03G0782100.
DR Gramene; TraesROB_scaffold_023913_01G000300.1; TraesROB_scaffold_023913_01G000300.1; TraesROB_scaffold_023913_01G000300.
DR Gramene; TraesWEE_scaffold_025461_01G000300.1; TraesWEE_scaffold_025461_01G000300.1; TraesWEE_scaffold_025461_01G000300.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000019116; Chromosome 5A.
DR Proteomes; UP000815260; Chromosome 5A.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650:SF45; DIACYLGLYCEROL O-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 51..74
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 220..248
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 72..294
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 352..467
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 481 AA; 52336 MW; E68BAC86EF52C942 CRC64;
MGDSGTNGSH SVAALPTSPL LPIRTPRKAP ADDPTADGEP VTPTARLMEP IYIVVTLGLG
CPVNLPVFSA GIAAQLARYP RFCSIQVTDE ANGGNPRWVR TVVNVDDHTI IPTLDPVAVA
ADPDRAVEDY LASLPALPMD RSRPLWEFHF LDFPTSEATS TAVIRVHHSL GDGTTLIALL
LASARSAADP TRLPAMPEQP ARTGAIYAPR PRSKGGVLAF LAWAWSYLVL AWNTMVDVTL
FAATIAFLRD PHTPFKYVDH GAASSSRRRF VHRSLPLEDV KFIKSAMNCT VNDVLVGATS
AALSRYFFRK SGANNTSKIC LRSILLVDTR PTTSLQTYVD MIDSGKSNDV DWGNQLGYML
LPFHLAMHDD PLAYVRKAKK TVDRKKSSLE VIFTCKMGES FLKMFGLKAG AFIFGRMFAN
TTLAFSNLVG PSEQIEFYGH PVVFIAPSVY GAPQALLVQC QSYNSTIMDG ASRLSTSVKQ
E
//
