ID A0A3B6LKQ2_WHEAT Unreviewed; 499 AA.
AC A0A3B6LKQ2;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5B02G169500.1};
RN [1] {ECO:0000313|EnsemblPlants:TraesCS5B02G169500.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5B02G169500.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5B02G169500.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR AlphaFoldDB; A0A3B6LKQ2; -.
DR SMR; A0A3B6LKQ2; -.
DR STRING; 4565.A0A3B6LKQ2; -.
DR PaxDb; 4565-Traes_5BL_AE25D84FC-1; -.
DR EnsemblPlants; TraesCS5B02G169500.1; TraesCS5B02G169500.1; TraesCS5B02G169500.
DR Gramene; TraesCS5B02G169500.1; TraesCS5B02G169500.1; TraesCS5B02G169500.
DR Gramene; TraesCS5B03G0456900.1; TraesCS5B03G0456900.1.CDS; TraesCS5B03G0456900.
DR OMA; RGYKAHA; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000019116; Chromosome 5B.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 28..499
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5017099868"
FT DOMAIN 30..490
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 417
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 468
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 477
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 499 AA; 53302 MW; A1195DE806532AA5 CRC64;
MGPSKACMSA YLIAVLCLVA GARNAAAFNY ADALDKAVLF FEAQRSGKLP PGQRVAWRAD
SALSDGNASN VDLVGGYYDA GDNVKFGLPM AFMVTMLSWS VVEFGGAMPG GQLANAEAAV
RWGSDYLLKA ATATPGALYV QVADPYQDHR CWERPEDMDT PRAVYKVTPQ SPGSDVAGET
AAALAAASLV FRAREPAYSS RLLRAARQVF DFADRYRGSY SDSLSSVVCP FYCSYSGYQD
ELLWAATWLH LASSPSPSSQ GVYMSYINSN GHTLGAEQDS YTVSWDDKRA ATKVLLSKVL
LQNRVEGLRG YKAHADKYIC SLVPGAGGFQ SQYTPGGLLF KEGDSNMEYV TSTAFLLLTY
AKYLGSTGGA ASCGATAVTP STLVSLAKKQ VDYILGANPA GMSYMVGFGA RYPRRVHHRG
ASMPSVRDHP ARIGCDEGFH YLHSSDPDRN VLVGAVVGGP DQSDSYSDSR DNYAQAEPST
YTNAPLVGAL AFFSGARSA
//
