ID A0A3B6LMF5_WHEAT Unreviewed; 773 AA.
AC A0A3B6LMF5;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Subtilisin-like protease {ECO:0008006|Google:ProtNLM};
GN ORFNames=CFC21_103549 {ECO:0000313|EMBL:KAF7102408.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5B02G209500.1};
RN [1] {ECO:0000313|EMBL:KAF7102408.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7102408.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5B02G209500.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5B02G209500.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5B02G209500.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7102408.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7102408.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase S8 family.
CC {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240}.
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DR EMBL; CM022230; KAF7102408.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6LMF5; -.
DR SMR; A0A3B6LMF5; -.
DR STRING; 4565.A0A3B6LMF5; -.
DR PaxDb; 4565-Traes_5BL_B58E08F2F-1; -.
DR EnsemblPlants; TraesCS5B02G209500.1; TraesCS5B02G209500.1; TraesCS5B02G209500.
DR Gramene; TraesCLE_scaffold_065333_01G000100.1; TraesCLE_scaffold_065333_01G000100.1; TraesCLE_scaffold_065333_01G000100.
DR Gramene; TraesCS5B02G209500.1; TraesCS5B02G209500.1; TraesCS5B02G209500.
DR Gramene; TraesCS5B03G0556400.1; TraesCS5B03G0556400.1.CDS; TraesCS5B03G0556400.
DR Gramene; TraesKAR5B01G0248310.1; cds.TraesKAR5B01G0248310.1; TraesKAR5B01G0248310.
DR Gramene; TraesROB_scaffold_054977_01G000400.1; TraesROB_scaffold_054977_01G000400.1; TraesROB_scaffold_054977_01G000400.
DR Gramene; TraesWEE_scaffold_037143_01G000100.1; TraesWEE_scaffold_037143_01G000100.1; TraesWEE_scaffold_037143_01G000100.
DR OrthoDB; 11910at2759; -.
DR Proteomes; UP000019116; Chromosome 5B.
DR Proteomes; UP000815260; Chromosome 7B.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd02120; PA_subtilisin_like; 1.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 2.60.40.2310; -; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 3.30.70.80; Peptidase S8 propeptide/proteinase inhibitor I9; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PROPROTEIN CONVERTASE SUBTILISIN/KEXIN; 1.
DR PANTHER; PTHR10795:SF335; SUBTILISIN-LIKE PROTEASE SBT1.8; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..773
FT /note="Subtilisin-like protease"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039992980"
FT DOMAIN 32..106
FT /note="Inhibitor I9"
FT /evidence="ECO:0000259|Pfam:PF05922"
FT DOMAIN 135..587
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 377..465
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT DOMAIN 669..768
FT /note="Subtilisin-like protease fibronectin type-III"
FT /evidence="ECO:0000259|Pfam:PF17766"
FT REGION 198..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 143
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 219
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 550
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 773 AA; 79757 MW; 03B5C8493DE0C57F CRC64;
MQQPPRLLLL LLLLCLAPSS ALASGGDEGA ATYIVYLNPA LKPSPYATHL HWHHAHLDAL
SVDPERHLLY SYTTAAPSAF AARLLPSHVT ELRGHPAVAS VHEDVLHPLH TTRSPSFLHL
PPYSAPAPNA DGGSSDVIVG VLDTGVWPES PSFVDAGLSP VPSRWRGSCE TNATDFPSSM
CNRKLIGARG FFRGFGAGGR NGSSHGTTEL SSPRDHDGHG THTASTAAGA VVADASLLGY
AHGTARGMAP GARVAAYKVC WRQGCFSSDI LAGMEQAIED GVDVLSLSLG GGSYPLSRDP
IAIGALAATR RGIVVACSAG NSGPAPSSLV NTAPWIITVG AGTLDRNFPA YAKLGNGETH
AGMSLYSGDG LCDDKFPLVY NKGIRPGSNA SKLCMSGTLD AAAVKGKVVL CDRGGNSRVE
KGQVVKLAGG VGMVLANTAQ SGEEVVADSH LLPAVAVGAK SGDAIRTYVE SDAGAEVALS
FAGTAVDVHP APVVAAFSSR GPNRQVAQLL KPDVIGPGVN ILAGWTGSVG PTGLTIDERR
PAFNILSGTS MSCPHISGLA AFVKAAHPDW SPSAIKSALM TTAYTVDNTG SPLLDAAGAN
ATASATPWSF GSGHVDPVKA LSPGLVYDTS IDDYVAFLCT VGGASPRQVQ AITGAPNATC
QRKLSSPGDL NYPSFSVVFG LRKSRTTVKY HRELTNVGAA GSVYAAKVTG PPSIVVSVKP
ARLVFKKAGD KLRYTVAFKS TAEGGPMDAA FGWLTWSSGE QDVRSPISYT WGM
//
