ID A0A3B6LR15_WHEAT Unreviewed; 531 AA.
AC A0A3B6LR15;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=CFC21_072807 {ECO:0000313|EMBL:KAF7066882.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5B02G323300.1};
RN [1] {ECO:0000313|EMBL:KAF7066882.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7066882.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5B02G323300.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5B02G323300.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5B02G323300.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7066882.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7066882.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; CM022224; KAF7066882.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6LR15; -.
DR SMR; A0A3B6LR15; -.
DR STRING; 4565.A0A3B6LR15; -.
DR PaxDb; 4565-Traes_5BL_699201983-1; -.
DR EnsemblPlants; TraesCS5B02G323300.1; TraesCS5B02G323300.1; TraesCS5B02G323300.
DR Gramene; TraesCAD_scaffold_012642_01G000300.1; TraesCAD_scaffold_012642_01G000300.1; TraesCAD_scaffold_012642_01G000300.
DR Gramene; TraesCS5B02G323300.1; TraesCS5B02G323300.1; TraesCS5B02G323300.
DR Gramene; TraesCS5B03G0815700.1; TraesCS5B03G0815700.1.CDS; TraesCS5B03G0815700.
DR Gramene; TraesPAR_scaffold_010152_01G000400.1; TraesPAR_scaffold_010152_01G000400.1; TraesPAR_scaffold_010152_01G000400.
DR OMA; YLINCHP; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000019116; Chromosome 5B.
DR Proteomes; UP000815260; Chromosome 5B.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF114; ENDOGLUCANASE 2; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 25..531
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5040525183"
FT DOMAIN 61..517
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT REGION 33..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 449
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
SQ SEQUENCE 531 AA; 57319 MW; 0FD2371E5DC4B076 CRC64;
MGSRTKGCCG WLIVALVLAL VATAAVVAIM KRKPGGGGGG RSRPPKPLPV PGPPGDIDSK
YGDALGVALQ FFQVQKAGKL VDNQIPWRGD SALNDGKEAK LDLSKGLYDA GDHMKFTFPM
AFTATVLAWS VLEYGDQMTA AKQLDPALDA LRWVTDFLIA AHPTDNVFYI QVGDPDLDHD
CWQRPETMTE KRPLTQINPK NPGSDIAAEA AAAMAAASMV FKSSDTTYSD SLLQHAQKLF
TFADTYKGIA SDTYPKLQKY YNSTGYVDEL LWAASWLYHA TGDQTYLSYV TVQNGKDYAD
WGRPTWFSWD DKLPGTQILL SRVNFFGSKE ISNAENSGLK MYRDTAEAVI CGLLPDSPSA
TGSRTSGGLV WISGWNSLQH GINAAFLAVV YSDYMLTSQT AAVECSGKYF SPTDIRNFAI
SQANYVLGDN PMKLSYLVGY GSSYPQQVHH RGASIPADAK TGCKGFEYLH SSSPNPNVAT
GAVVGGPFQN DSFVDLRDNA VQTESSTYNS GTFVGLLAGL VTTSSIAKTF T
//
