ID A0A3B6MJW6_WHEAT Unreviewed; 446 AA.
AC A0A3B6MJW6;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=phosphopyruvate hydratase {ECO:0000256|ARBA:ARBA00012058};
DE EC=4.2.1.11 {ECO:0000256|ARBA:ARBA00012058};
GN ORFNames=CFC21_075567 {ECO:0000313|EMBL:KAF7070004.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5D02G020100.1};
RN [1] {ECO:0000313|EMBL:KAF7070004.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7070004.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5D02G020100.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5D02G020100.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5D02G020100.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7070004.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7070004.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 4/5. {ECO:0000256|ARBA:ARBA00005031}.
CC -!- SIMILARITY: Belongs to the enolase family.
CC {ECO:0000256|ARBA:ARBA00009604}.
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DR EMBL; CM022225; KAF7070004.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6MJW6; -.
DR SMR; A0A3B6MJW6; -.
DR STRING; 4565.A0A3B6MJW6; -.
DR EnsemblPlants; TraesCS5D02G020100.1; TraesCS5D02G020100.1; TraesCS5D02G020100.
DR Gramene; TraesCAD_scaffold_096119_01G000300.1; TraesCAD_scaffold_096119_01G000300.1; TraesCAD_scaffold_096119_01G000300.
DR Gramene; TraesCLE_scaffold_072679_01G000300.1; TraesCLE_scaffold_072679_01G000300.1; TraesCLE_scaffold_072679_01G000300.
DR Gramene; TraesCS5D02G020100.1; TraesCS5D02G020100.1; TraesCS5D02G020100.
DR Gramene; TraesKAR5D01G0009080.1; cds.TraesKAR5D01G0009080.1; TraesKAR5D01G0009080.
DR Gramene; TraesPAR_scaffold_109336_01G000100.1; TraesPAR_scaffold_109336_01G000100.1; TraesPAR_scaffold_109336_01G000100.
DR Gramene; TraesROB_scaffold_107508_01G000300.1; TraesROB_scaffold_107508_01G000300.1; TraesROB_scaffold_107508_01G000300.
DR Gramene; TraesWEE_scaffold_105116_01G000100.1; TraesWEE_scaffold_105116_01G000100.1; TraesWEE_scaffold_105116_01G000100.
DR OMA; RCQLTGD; -.
DR UniPathway; UPA00109; UER00187.
DR Proteomes; UP000019116; Chromosome 5D.
DR Proteomes; UP000815260; Chromosome 5D.
DR GO; GO:0000015; C:phosphopyruvate hydratase complex; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0004634; F:phosphopyruvate hydratase activity; IBA:GO_Central.
DR GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR CDD; cd03313; enolase; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR HAMAP; MF_00318; Enolase; 1.
DR InterPro; IPR000941; Enolase.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR020810; Enolase_C.
DR InterPro; IPR020809; Enolase_CS.
DR InterPro; IPR020811; Enolase_N.
DR NCBIfam; TIGR01060; eno; 1.
DR PANTHER; PTHR11902; ENOLASE; 1.
DR PANTHER; PTHR11902:SF50; ENOLASE; 1.
DR Pfam; PF00113; Enolase_C; 1.
DR Pfam; PF03952; Enolase_N; 1.
DR PIRSF; PIRSF001400; Enolase; 1.
DR PRINTS; PR00148; ENOLASE.
DR SFLD; SFLDF00002; enolase; 1.
DR SFLD; SFLDG00178; enolase; 1.
DR SMART; SM01192; Enolase_C; 1.
DR SMART; SM01193; Enolase_N; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
DR PROSITE; PS00164; ENOLASE; 1.
PE 3: Inferred from homology;
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116}.
FT DOMAIN 5..140
FT /note="Enolase N-terminal"
FT /evidence="ECO:0000259|SMART:SM01193"
FT DOMAIN 148..442
FT /note="Enolase C-terminal TIM barrel"
FT /evidence="ECO:0000259|SMART:SM01192"
FT ACT_SITE 216
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-1"
FT ACT_SITE 354
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-1"
FT BINDING 164
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 173
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 302
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 329
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 381..384
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
FT BINDING 405
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001400-2"
SQ SEQUENCE 446 AA; 48004 MW; 813E3ECC0151B85C CRC64;
MAATIQSVKA RQIFDSRGNP TVEVDVCCSD GTFARAAVPS GASTGVYEAL ELRDGGSDYL
GKGVSKAVDN VNSIIAPALI GKDPTAQTEL DNFMVQQLDG TKNEWGWCKQ KLGANAILAV
SLAVCKAGAS VKKIPLYQHI ANLAGNKQLV LPVPAFNVIN GGSHAGNKLA MQEFMILPTG
AASFKEAMKM GVEVYHNLKS VIKKKYGQDA TNVGDEGGFA PNIQENKEGL ELLKTAIEKA
GYTGKVVIGM DVAASEFYND KDKTYDLNFK EENNDGSQKI SGDSLKNVYK SFVSEYPIVS
IEDPFDQDDW VHYAKMTEEC GVEVQIVGDD LLVTNPTRVA KAIKEKSCNA LLLKVNQIGS
VTESIEAVKM SKHAGWGVMT SHRSGETEDT FIADLAVGLS TGQIKTGAPC RSERLAKYNQ
LLRIEEELGA AAVYAGLKFR APVEPY
//
