ID A0A3B6MM88_WHEAT Unreviewed; 228 AA.
AC A0A3B6MM88;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=E3 ubiquitin-protein ligase RMA {ECO:0000256|RuleBase:RU369090};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU369090};
DE AltName: Full=Protein RING membrane-anchor {ECO:0000256|RuleBase:RU369090};
DE AltName: Full=RING-type E3 ubiquitin transferase RMA {ECO:0000256|RuleBase:RU369090};
GN ORFNames=CFC21_075997 {ECO:0000313|EMBL:KAF7070478.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5D02G060200.1.cds1};
RN [1] {ECO:0000313|EMBL:KAF7070478.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7070478.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5D02G060200.1.cds1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5D02G060200.1.cds1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5D02G060200.1.cds1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7070478.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7070478.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: E3 ubiquitin-protein ligase. {ECO:0000256|RuleBase:RU369090}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU369090};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369090}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|RuleBase:RU369090}; Single-pass type IV membrane protein
CC {ECO:0000256|RuleBase:RU369090}.
CC -!- DOMAIN: The RING-type zinc finger domain is responsible for E3 ligase
CC activity. {ECO:0000256|RuleBase:RU369090}.
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DR EMBL; CM022225; KAF7070478.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6MM88; -.
DR STRING; 4565.A0A3B6MM88; -.
DR EnsemblPlants; TraesCS5D02G060200.1; TraesCS5D02G060200.1.cds1; TraesCS5D02G060200.
DR Gramene; TraesCAD_scaffold_078243_01G000200.1; TraesCAD_scaffold_078243_01G000200.1; TraesCAD_scaffold_078243_01G000200.
DR Gramene; TraesCLE_scaffold_081734_01G000200.1; TraesCLE_scaffold_081734_01G000200.1; TraesCLE_scaffold_081734_01G000200.
DR Gramene; TraesCS5D02G060200.1; TraesCS5D02G060200.1.cds1; TraesCS5D02G060200.
DR Gramene; TraesCS5D03G0148700.1; TraesCS5D03G0148700.1.CDS1; TraesCS5D03G0148700.
DR Gramene; TraesKAR5D01G0043080.1; cds.TraesKAR5D01G0043080.1; TraesKAR5D01G0043080.
DR Gramene; TraesPAR_scaffold_067324_01G000100.1; TraesPAR_scaffold_067324_01G000100.1; TraesPAR_scaffold_067324_01G000100.
DR Gramene; TraesRN5D0100156300.1; TraesRN5D0100156300.1; TraesRN5D0100156300.
DR Gramene; TraesROB_scaffold_087309_01G000100.1; TraesROB_scaffold_087309_01G000100.1; TraesROB_scaffold_087309_01G000100.
DR Gramene; TraesWEE_scaffold_071632_01G000100.1; TraesWEE_scaffold_071632_01G000100.1; TraesWEE_scaffold_071632_01G000100.
DR OrthoDB; 397219at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000019116; Chromosome 5D.
DR Proteomes; UP000815260; Chromosome 5D.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0044390; F:ubiquitin-like protein conjugating enzyme binding; IBA:GO_Central.
DR GO; GO:0071712; P:ER-associated misfolded protein catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR045103; RNF5/RNF185-like.
DR InterPro; IPR018957; Znf_C3HC4_RING-type.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR12313:SF60; E3 UBIQUITIN-PROTEIN LIGASE RMA; 1.
DR PANTHER; PTHR12313; E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED; 1.
DR Pfam; PF00097; zf-C3HC4; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 4: Predicted;
KW Endoplasmic reticulum {ECO:0000256|RuleBase:RU369090};
KW Membrane {ECO:0000256|RuleBase:RU369090};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU369090};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU369090};
KW Transmembrane {ECO:0000256|RuleBase:RU369090};
KW Transmembrane helix {ECO:0000256|RuleBase:RU369090};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|RuleBase:RU369090};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU369090};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT TRANSMEM 210..227
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369090"
FT DOMAIN 37..85
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
SQ SEQUENCE 228 AA; 24734 MW; F765DB28207C730D CRC64;
MTEVTKEKCR PADKLQHSIN DAIVGVPGGA TAACWDCSIC LEVASEPVVT LCGHLYCWPC
IFRWLTTSSK SKSRASPSAR CPVCKAAVSE DHLVPLYGRA RATTLSPAGG RGGGVCQVPC
RSPVPGPNVE ERLHGPDSDD GGNFYYYYDN VGINGLDCSN AERLLGGIAL AVLLPWATRG
AGGRPPPPSL YRDGEGWRMA RQQRRVARRL RQIWVFLAMV AFLCFLLL
//
