ID A0A3B6N0X9_WHEAT Unreviewed; 1847 AA.
AC A0A3B6N0X9;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=CFC21_080580 {ECO:0000313|EMBL:KAF7075840.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS5D02G498900.1};
RN [1] {ECO:0000313|EMBL:KAF7075840.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7075840.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS5D02G498900.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS5D02G498900.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS5D02G498900.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7075840.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7075840.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; CM022225; KAF7075840.1; -; Genomic_DNA.
DR SMR; A0A3B6N0X9; -.
DR STRING; 4565.A0A3B6N0X9; -.
DR EnsemblPlants; TraesCS5D02G498900.1; TraesCS5D02G498900.1; TraesCS5D02G498900.
DR Gramene; TraesCLE_scaffold_049682_01G000200.1; TraesCLE_scaffold_049682_01G000200.1; TraesCLE_scaffold_049682_01G000200.
DR Gramene; TraesCS5D02G498900.1; TraesCS5D02G498900.1; TraesCS5D02G498900.
DR Gramene; TraesCS5D03G1093100.1; TraesCS5D03G1093100.1.CDS; TraesCS5D03G1093100.
DR Gramene; TraesKAR5D01G0386720.1; cds.TraesKAR5D01G0386720.1; TraesKAR5D01G0386720.
DR Gramene; TraesROB_scaffold_078606_01G000100.1; TraesROB_scaffold_078606_01G000100.1; TraesROB_scaffold_078606_01G000100.
DR Gramene; TraesWEE_scaffold_043890_01G000200.1; TraesWEE_scaffold_043890_01G000200.1; TraesWEE_scaffold_043890_01G000200.
DR OMA; VMELMMV; -.
DR OrthoDB; 7998at2759; -.
DR Proteomes; UP000019116; Chromosome 5D.
DR Proteomes; UP000815260; Chromosome 5D.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-KW.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:InterPro.
DR CDD; cd02584; RNAP_II_Rpb1_C; 1.
DR CDD; cd02733; RNAP_II_RPB1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.1360.140; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 2.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR000684; RNA_pol_II_repeat_euk.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR007073; RNA_pol_Rpb1_7.
DR InterPro; IPR038593; RNA_pol_Rpb1_7_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR Pfam; PF04990; RNA_pol_Rpb1_7; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
DR PROSITE; PS00115; RNA_POL_II_REPEAT; 5.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 246..552
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 158..178
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1620..1665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1685..1847
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 698..725
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1685..1835
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1847 AA; 206051 MW; 68923309C3D679CF CRC64;
MDARFAYSPA ETARVQLVQF GILSPDEIRQ MSVAVIEHAE TMEKGKPKPG GLSDPRLGTI
DRRTKCETCM AGMAECPGHF GHLELAKPMF HIGFIKTVLS IMRCVCFNCS KILADEDDEK
DGKFKQALRI KNPKNRLRKI QEACKGKKVC AAGEDDLEGQ DQMDTDEPVK KRGGCGAQQP
KITVDGMKMV AEFKATNKKN GDQDQLPEPV ERKQILSAER VLNVLKRISD EDCLLLGLDP
KYARPDWMIL QVLPIPPPPV RPSVMMDTSS RSEDDLTHQL AMIIRHNENL RRQERNGVPA
HIITEFAQLL QFHIATYFDN DLPGQPRATQ RSGRPIKSIC SRLKAKEGRV RGNLMGKRVD
FSARTVITPD PNINIDELGV PWSIALNLTY PETVTPYNIE RLKELVENGP HPPPGKTGAK
YIIREDGQRL DLRYVKKSSD QHLELGYKVE RHLIDGDFVL FNRQPSLHKM SIMGHRIKIM
PYSTFRLNLS VTSPYNADFD GDEMNMHVPQ SFETRAEVME LMMVPKCIVS PQANRPVMGI
VQDTLLGCRK ITKRDTLIEK DVFMNILMWW EDFDGKVPAP AILKPRPIWT GKQVFNLIIP
KRINLIRFSA WHDEEESRFI TPGDTVVRIE KGELLSGILC KKTLGTSMGS LIHVIWEEVG
PDAARKFLGH TQWLVNYWLL QHGFSIGIGD TIADAGTMKK ISDTISKAQD DVNELIKEAR
DNKLEPEPGR TMMESFENKV NQVLNKARDD SGSSAQQSLS ESNNLKAMVT AGSKGSFINI
SQMTACVGQQ NVEGKRIPFG FAGRTLPHFT QNDDSPESRG FVENSYLRGL TPQEFFFHAM
GGREGLIDTA VKTSETGYIQ RRLVKAMEDI MVKYDGTVRN SLGDVIQFLY GEDGMDAVWI
EPQKLDSLGM KKAEFDNVFR YELDDENWRP TYMSPEDADV LKTVPDIRLV FEAEFQKLEA
DRLQLGTEIA TTGDITWPMP VNLKRLIWNA QKTFKIDLRR PSDMHPMEIV EAMDKLQERL
KVVPGDDAMS IEAQKNATLL FNILLRSTFA SKRVLKEYRL TRESFEWVIG EIESRFLQSL
VAPGEMIGCV AAQSIGEPAT QMTLNTFHYA GVSAKNVTLG VPRLREIINV AKKIKTPSLS
VFLKPEVSKK KELAKNVQCA LEYTTLRSVT HATEIWYDPD PLRTTIEEDV EFVKSYYEMP
DEDIDPDKMS PWLLRIELNR EMMVDKRLSM AEIAEKIKCE FDDDLSCIFN DDNADKLILR
IRIKNDDEVP KQDEDDDVFL KKVESNMLTE MALGGIPDIN KVFIKERKVN KFDENDGFTQ
KSDNKDKNKE WMLDTEGVNL LAVMCHEDVD TTRTTSNHLI EVIEVLGIEA VRRSLLDELR
AVISFDGSYV NYRHLAILCD TMTYRGHLMA ITRHGINRND TGPLMRCSFE ETVDILLDAA
MYAESDHLRG VTENIMLGQL APIGTGGCEL YLNDKMLEQA IELQLPSFVE GLDYGMTPAP
SPISGMMSPV PSPNFRTSPV TDHALQFSPY VGGLAFSPVP SNYSTSSGSG YCPSSPVFSP
GPGGQGYSPV SPVYSPLSPI YTPTSLSYSP VSPIHSPESP VYSPASPVFR PTSLQYSPTS
PVYSPTSLEY SPTSPSYSPT SHAYSPTSPS YSPTSHAYSP TSPSYSPTSP VYSPASFSTS
PYYNPTSPSY SPTSHSYSPT SPRYSPASPS YSPTSPNYSP TSPAYSPTSP SYSPTSPSYS
PTSPSYSPTS PSYSPSRAYS PSSPMMMSPY SQLPPNYSLA SPTYSPTSPS YAAQPSPSYS
PTSPCTASGG PSPDYSLTSP NYSPSASYSP PSTEPQTSDK DDDKSAP
//
