ID A0A3B6RAS7_WHEAT Unreviewed; 518 AA.
AC A0A3B6RAS7;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=CFC21_096439 {ECO:0000313|EMBL:KAF7094093.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS7A02G144700.1};
RN [1] {ECO:0000313|EMBL:KAF7094093.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7094093.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS7A02G144700.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS7A02G144700.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS7A02G144700.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7094093.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7094093.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; CM022229; KAF7094093.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6RAS7; -.
DR SMR; A0A3B6RAS7; -.
DR STRING; 4565.A0A3B6RAS7; -.
DR PaxDb; 4565-Traes_7AS_FD24971A9-1; -.
DR EnsemblPlants; TraesCS7A02G144700.1; TraesCS7A02G144700.1; TraesCS7A02G144700.
DR Gramene; TraesCAD_scaffold_072883_01G000100.1; TraesCAD_scaffold_072883_01G000100.1; TraesCAD_scaffold_072883_01G000100.
DR Gramene; TraesCLE_scaffold_001400_01G000100.1; TraesCLE_scaffold_001400_01G000100.1; TraesCLE_scaffold_001400_01G000100.
DR Gramene; TraesCS7A02G144700.1; TraesCS7A02G144700.1; TraesCS7A02G144700.
DR Gramene; TraesCS7A03G0339600.1; TraesCS7A03G0339600.1.CDS; TraesCS7A03G0339600.
DR Gramene; TraesKAR7A01G0068320.1; cds.TraesKAR7A01G0068320.1; TraesKAR7A01G0068320.
DR Gramene; TraesPAR_scaffold_062973_01G000200.1; TraesPAR_scaffold_062973_01G000200.1; TraesPAR_scaffold_062973_01G000200.
DR Gramene; TraesRN7A0100309800.1; TraesRN7A0100309800.1; TraesRN7A0100309800.
DR Gramene; TraesROB_scaffold_062546_01G000100.1; TraesROB_scaffold_062546_01G000100.1; TraesROB_scaffold_062546_01G000100.
DR Gramene; TraesWEE_scaffold_169447_01G000100.1; TraesWEE_scaffold_169447_01G000100.1; TraesWEE_scaffold_169447_01G000100.
DR OMA; YLVKAHT; -.
DR Proteomes; UP000019116; Chromosome 7A.
DR Proteomes; UP000815260; Chromosome 7A.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF107; ENDOGLUCANASE 21; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 28..518
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5040519900"
FT DOMAIN 41..504
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 431
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
SQ SEQUENCE 518 AA; 57193 MW; 1F4DEAD7E75FB144 CRC64;
MATRIKILTS LAICAALVLV LLPFTAADGG GGAKGAAPFD YKKALHSSLL YFEAQRSGHL
PHNQRVKWRG HSGLADGLQQ GVDLVGGYYD AGDNVKFGLP MAFTVTMLSW SAMEFADDIA
AAGEWRHVLE AIKWGTDYFV KAHTEPYVLW AEVGDGDTDH YCWQRPEDMT TSRQAYKVDS
DNPGSDLVGE TAAALAAASM VFRRSNPHYA QVLLHHAEQL FEFGDKYRGK YDSSIGEAYS
YYASVSGYGD EMLWAALWLH RATGKASYLD YAVDMADEFG GTTWAITEFS WDVKYAGLQI
LATKVLLEGK HPEHKATLEK YKAKAEHYLC ACLNKNGDAG NVNRTAGGML FVRQWNNMQY
VTNAAFLLTV YSRYLTSAGQ ELPVLQCPDG PGHADELRAL GRAQTDYVLG ANPAGVSYLV
GYGQRFPRRM HHRGASIVSH RGDGRFIGCM QGYDNWFLRR GSNPNVVVGA IVGGPDHLDR
FRDRRDNYMQ TEACTYNTAP MVGIFAHLHG ETAITEKS
//
