ID A0A3B6RFP5_WHEAT Unreviewed; 495 AA.
AC A0A3B6RFP5;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=2-isopropylmalate synthase {ECO:0000256|ARBA:ARBA00012973};
DE EC=2.3.3.13 {ECO:0000256|ARBA:ARBA00012973};
GN ORFNames=CFC21_096795 {ECO:0000313|EMBL:KAF7094490.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS7A02G179500.1};
RN [1] {ECO:0000313|EMBL:KAF7094490.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7094490.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS7A02G179500.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS7A02G179500.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS7A02G179500.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7094490.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7094490.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 1/4.
CC {ECO:0000256|ARBA:ARBA00004689}.
CC -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC family. {ECO:0000256|RuleBase:RU003523}.
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DR EMBL; CM022229; KAF7094490.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6RFP5; -.
DR SMR; A0A3B6RFP5; -.
DR STRING; 4565.A0A3B6RFP5; -.
DR PaxDb; 4565-Traes_7AS_12729F868-1; -.
DR EnsemblPlants; TraesCS7A02G179500.1; TraesCS7A02G179500.1; TraesCS7A02G179500.
DR Gramene; TraesCAD_scaffold_098636_01G000200.1; TraesCAD_scaffold_098636_01G000200.1; TraesCAD_scaffold_098636_01G000200.
DR Gramene; TraesCS7A02G179500.1; TraesCS7A02G179500.1; TraesCS7A02G179500.
DR Gramene; TraesCS7A03G0418900.1; TraesCS7A03G0418900.1.CDS; TraesCS7A03G0418900.
DR Gramene; TraesKAR7A01G0092840.1; cds.TraesKAR7A01G0092840.1; TraesKAR7A01G0092840.
DR Gramene; TraesPAR_scaffold_073557_01G000200.1; TraesPAR_scaffold_073557_01G000200.1; TraesPAR_scaffold_073557_01G000200.
DR Gramene; TraesWEE_scaffold_060575_01G000200.1; TraesWEE_scaffold_060575_01G000200.1; TraesWEE_scaffold_060575_01G000200.
DR OMA; DTVGVMW; -.
DR OrthoDB; 375at2759; -.
DR Proteomes; UP000019116; Chromosome 7A.
DR Proteomes; UP000815260; Chromosome 7A.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0003852; F:2-isopropylmalate synthase activity; IBA:GO_Central.
DR GO; GO:0009098; P:leucine biosynthetic process; IBA:GO_Central.
DR CDD; cd07940; DRE_TIM_IPMS; 1.
DR Gene3D; 1.10.238.260; -; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR000891; PYR_CT.
DR PANTHER; PTHR10277:SF9; 2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED; 1.
DR PANTHER; PTHR10277; HOMOCITRATE SYNTHASE-RELATED; 1.
DR Pfam; PF00682; HMGL-like; 1.
DR SUPFAM; SSF51569; Aldolase; 1.
DR PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR PROSITE; PS50991; PYR_CT; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Transferase {ECO:0000256|RuleBase:RU003523}.
FT DOMAIN 67..338
FT /note="Pyruvate carboxyltransferase"
FT /evidence="ECO:0000259|PROSITE:PS50991"
FT REGION 468..495
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 495 AA; 53304 MW; 46B6021097FDDCA7 CRC64;
MSTAATTFGS PNLAPASHSF PSCRLLSHGF TVAANRRVSH ASARASPAAP RRRPEYVPSR
IDDPNYIRIF DTTLRDGEQS PGATMTSAEK LVVARQLALL GVDVIDAGFP ASSPDDFRAV
RSIAIEVGNT PVGGHIPVIG GLARCNKRDI DAAWDAVKHA RRPRINTFIA TSEIHMQHKL
RKTPEQVVAI AREMVAYAGA LGCPDVEFCP EDSGRSNKEF LYHILEEVIQ AGATTITIAD
TVGSNLPVEY GKLIADIKAN TPGIDNAIIS THCHNDLGLA TANTLAGAYA GARQLEVTIN
GIGERAGNAA LEEVVMALKC RPELLDGMYT GINTHHITMT SKMVQEYSGL HVQPHKAIVG
ANAFAHESGI HQDGMLKYKG TYEIISPDDI GLTRANDSGI VLGKLSGRHA VRSVLVELGY
EISDNEFEDF FKRYKEVAEK KKRINDKDIK ALLYDEIFKP KLNPSVGSNN MPGGASGHVL
GSLETAHGTS TSQQQ
//
