ID A0A3B6SGC0_WHEAT Unreviewed; 591 AA.
AC A0A3B6SGC0;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=SAM-dependent MTase RsmB/NOP-type domain-containing protein {ECO:0000259|PROSITE:PS51686};
GN ORFNames=CFC21_103903 {ECO:0000313|EMBL:KAF7102837.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS7B02G277100.1};
RN [1] {ECO:0000313|EMBL:KAF7102837.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7102837.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS7B02G277100.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS7B02G277100.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EnsemblPlants:TraesCS7B02G277100.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:KAF7102837.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7102837.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR EMBL; CM022230; KAF7102837.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B6SGC0; -.
DR SMR; A0A3B6SGC0; -.
DR STRING; 4565.A0A3B6SGC0; -.
DR EnsemblPlants; TraesCS7B02G277100.1; TraesCS7B02G277100.1; TraesCS7B02G277100.
DR Gramene; TraesCAD_scaffold_011813_01G000100.1; TraesCAD_scaffold_011813_01G000100.1; TraesCAD_scaffold_011813_01G000100.
DR Gramene; TraesCS7B02G277100.1; TraesCS7B02G277100.1; TraesCS7B02G277100.
DR Gramene; TraesKAR7B01G0332390.3; cds.TraesKAR7B01G0332390.3; TraesKAR7B01G0332390.
DR Gramene; TraesROB_scaffold_010200_01G000100.1; TraesROB_scaffold_010200_01G000100.1; TraesROB_scaffold_010200_01G000100.
DR OMA; GYTEEWL; -.
DR OrthoDB; 1077100at2759; -.
DR Proteomes; UP000019116; Chromosome 7B.
DR Proteomes; UP000815260; Chromosome 7B.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IBA:GO_Central.
DR CDD; cd21150; PUA_NSun6-like; 1.
DR Gene3D; 2.30.130.10; PUA domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR22807:SF34; TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 2.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF88697; PUA domain-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR PROSITE; PS50890; PUA; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 202..587
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 506
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 298..304
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 322
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 349
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 455
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 591 AA; 63834 MW; 31A8088421F185AC CRC64;
MDSSSASPAG AAAAAAAPAA GAAAATDRYT FRPGLRWQPE VEEYFASAYG RDHFARISQA
LAHPSCYSCI RVNTLKSSTD TVMQKLLKLV NENELSCGFN GLKIGEQNGG EQAHEGSYLV
HKCPYSGLEN VLFVRGSGPH ALHYNDQPGH SMKEIIVSRK CAESVLRGAQ VYVPGVLACS
AHVEKGDKVA VSVAIEQPVK NSGWAVGITR GIVLQGLQSD AHYEERKGLY IGQGIAAMSR
AGIFRVLHGV AVEMTERVYK LPSFNDVLEG EIFLQNLPSI VAAHVLDPQP GEQILDMCAA
PGGKTTAIAI LMRDKGEIVA LDRSHNKVMG ILKLADEMGL SCIRAYKLDA LKSVRKACEE
RNLGVADNCS EAIVTLAENS GPIRSIIGAR VTDAAEDSST AIVEQTDAKS YVSKADLRKN
LRRMRNGTGR SNCSGGRVED SKGFFPSSFD RVLLDAPCSA LGLRPRLFAG EETLESLRKN
ATYQRRLFDQ AVKLVRPGGI IVYSTCTINP GENEALVRYA LDTYKFLSLG SQHPKVGGPG
IIGSCKLPNK AYTEQWLTED EAQLVQRFDP SSSLDTMGFF IAKFNVGQKE D
//
