ID A0A3B6TIL6_WHEAT Unreviewed; 596 AA.
AC A0A3B6TIL6;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=methionine--tRNA ligase {ECO:0000256|ARBA:ARBA00012838};
DE EC=6.1.1.10 {ECO:0000256|ARBA:ARBA00012838};
DE AltName: Full=Methionyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030904};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS7D02G395100.1};
RN [1] {ECO:0000313|EnsemblPlants:TraesCS7D02G395100.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS7D02G395100.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS7D02G395100.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10;
CC Evidence={ECO:0000256|ARBA:ARBA00001234};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363039}.
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DR AlphaFoldDB; A0A3B6TIL6; -.
DR SMR; A0A3B6TIL6; -.
DR STRING; 4565.A0A3B6TIL6; -.
DR EnsemblPlants; TraesCS7D02G395100.1; TraesCS7D02G395100.1; TraesCS7D02G395100.
DR Gramene; TraesCS7D02G395100.1; TraesCS7D02G395100.1; TraesCS7D02G395100.
DR Gramene; TraesCS7D03G0931200.1; TraesCS7D03G0931200.1.CDS; TraesCS7D03G0931200.
DR Gramene; TraesWEE_scaffold_152553_01G000100.1; TraesWEE_scaffold_152553_01G000100.1; TraesWEE_scaffold_152553_01G000100.
DR OMA; AREENCT; -.
DR Proteomes; UP000019116; Chromosome 7D.
DR GO; GO:0017101; C:aminoacyl-tRNA synthetase multienzyme complex; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IBA:GO_Central.
DR GO; GO:0009791; P:post-embryonic development; IEA:UniProt.
DR GO; GO:0048608; P:reproductive structure development; IEA:UniProt.
DR CDD; cd07957; Anticodon_Ia_Met; 1.
DR CDD; cd00814; MetRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 2.20.28.20; Methionyl-tRNA synthetase, Zn-domain; 1.
DR HAMAP; MF_00098; Met_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR041872; Anticodon_Met.
DR InterPro; IPR023458; Met-tRNA_ligase_1.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR029038; MetRS_Zn.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00398; metG; 1.
DR PANTHER; PTHR45765; METHIONINE--TRNA LIGASE; 1.
DR PANTHER; PTHR45765:SF9; METHIONINE--TRNA LIGASE; 1.
DR Pfam; PF19303; Anticodon_3; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF57770; Methionyl-tRNA synthetase (MetRS), Zn-domain; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363039};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363039};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363039};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116}.
FT DOMAIN 18..412
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
FT DOMAIN 437..588
FT /note="Methionyl-tRNA synthetase anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF19303"
SQ SEQUENCE 596 AA; 67084 MW; 5A9C7131DEE3414B CRC64;
MSSGKGKAKV PVAGRRNVLV TSALPYVNNV PHLGNIIGCV LSADVFARYC RLRGHNVLYV
CGTDEYGTAT ENKALEEGCS PLEICDKYHA IHKEVYEWFN ISFDHFGRTS TPQQTEICQS
IFSKLLDNNW LSENTMQQLY CESCQRFLAD RFVVGSCPVE GCGNDTARGD QCDRCGRLLN
STELIDPRCK VCEGIPIVRD TDHLFLELPL LKEQLEKYID EASATGSWSQ NAVRITDAWL
KEGLRPRCIT RDLKWGVPVP HEKYKDKVFY VWFDAPIGYI SITACYTPEW EKWWKNPENV
ELYQFMGKDN VPFHTVMFPS ALLGTGENWT LMKNISVTEY LNYESGKFSK TKGIGVFGND
AKATNIPADV WRYYLLSNRP EASDAVFTWG GLQIKHNNEL LKNLGNFIYR VLSFIAKPEG
AGYGSIIPEA PNADIHPLSQ SLAETVGNLI QQYIDAMDKV KLKQGLKIAM AISSEGNAYL
QESQFWKLYK QDPASCTTVM KTSVGVVYLL ACLLEPFMPS FSKDVLQQLN LSPNEHISFA
DEKGESDKAK RPWDLIPSNH RIGEIVPLFT ELKDDAVASF RETFAGSQAE RNARAN
//
