ID A0A3B7MT20_9BACT Unreviewed; 507 AA.
AC A0A3B7MT20;
DT 16-JAN-2019, integrated into UniProtKB/TrEMBL.
DT 16-JAN-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Probable membrane transporter protein {ECO:0000256|RuleBase:RU363041};
GN ORFNames=D3H65_19930 {ECO:0000313|EMBL:AXY76116.1};
OS Paraflavitalea soli.
OC Bacteria; Bacteroidota; Chitinophagia; Chitinophagales; Chitinophagaceae;
OC Paraflavitalea.
OX NCBI_TaxID=2315862 {ECO:0000313|EMBL:AXY76116.1, ECO:0000313|Proteomes:UP000263900};
RN [1] {ECO:0000313|EMBL:AXY76116.1, ECO:0000313|Proteomes:UP000263900}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=5GH32-13 {ECO:0000313|EMBL:AXY76116.1,
RC ECO:0000313|Proteomes:UP000263900};
RA Weon H.-Y., Heo J., Kwon S.-W.;
RT "Genome sequencing of strain 6GH32-13.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin;
CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76;
CC Evidence={ECO:0000256|ARBA:ARBA00001156};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
CC {ECO:0000256|ARBA:ARBA00005010}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU363041};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU363041}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the 4-toluene sulfonate uptake permease (TSUP)
CC (TC 2.A.102) family. {ECO:0000256|RuleBase:RU363041}.
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DR EMBL; CP032157; AXY76116.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3B7MT20; -.
DR KEGG; pseg:D3H65_19930; -.
DR OrthoDB; 45564at2; -.
DR UniPathway; UPA00262; UER00222.
DR Proteomes; UP000263900; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004325; F:ferrochelatase activity; IEA:InterPro.
DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR028161; Met8-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR028281; Sirohaem_synthase_central.
DR InterPro; IPR006367; Sirohaem_synthase_N.
DR InterPro; IPR002781; TM_pro_TauE-like.
DR NCBIfam; TIGR01470; cysG_Nterm; 1.
DR PANTHER; PTHR35330; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR PANTHER; PTHR35330:SF1; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR Pfam; PF14824; Sirohm_synth_M; 1.
DR Pfam; PF01925; TauE; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU363041};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU363041};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244};
KW Reference proteome {ECO:0000313|Proteomes:UP000263900};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU363041};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU363041}.
FT TRANSMEM 221..242
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 262..285
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 330..347
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 359..380
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 428..451
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 457..475
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 487..506
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT DOMAIN 143..164
FT /note="Siroheme synthase central"
FT /evidence="ECO:0000259|Pfam:PF14824"
SQ SEQUENCE 507 AA; 55499 MW; A4024B2B1AE66ED3 CRC64;
MSTEQQKNGV PDATVIGQDA NYLFPVFLRL EVLEVLLVGA GNVGLEKLTA ILGNAPAARV
SVVATNVTAQ VKELAGAHEF VRIEERPFSE ADLEGKDLVI IAVNDRAVSQ LIRDAAREKK
ILVNAADKPE QCDFYLSSVV QKGNLKIAIS TNGKSPTAAK RIKEVLNNAL PAELNEVINN
LHVVRNRLNG NFEYKVKKLN ELTKILVERD TAEKERRWRK IATYSLVIFA LMLVGHFIFS
YLPLQQMADD TLKWYQTLDK NFHWMVMAGF LAQMVDGALG MGYGVTSTTI LLSMGVNLAA
ISGSIHTAEM FASGASGYSH YKFGNVNKKL FKILVIPGVI GAIIGAICLK EFGESHSKYL
RPILASYTMF LGLRILYNAF RKQQQKPKKF KRYGMLAGAG GFLDSFGGGG WGPIVTTTLI
TKGRSPRFVI GSVSLTEFFV TLASALTFFT LMGVSHWQVI AALILGGLIA APLAAKLAGK
LPRKTALILL GCLVIFWSVK IIIKIFQ
//
