ID A0A3D8GME3_9BACI Unreviewed; 753 AA.
AC A0A3D8GME3;
DT 16-JAN-2019, integrated into UniProtKB/TrEMBL.
DT 16-JAN-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=DRW41_17890 {ECO:0000313|EMBL:RDU35603.1};
OS Neobacillus piezotolerans.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Neobacillus.
OX NCBI_TaxID=2259171 {ECO:0000313|EMBL:RDU35603.1, ECO:0000313|Proteomes:UP000257144};
RN [1] {ECO:0000313|EMBL:RDU35603.1, ECO:0000313|Proteomes:UP000257144}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YLB-04 {ECO:0000313|EMBL:RDU35603.1,
RC ECO:0000313|Proteomes:UP000257144};
RA Yu L., Tang X.;
RT "Bacillus sp. YLB-04 draft genome sequence.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDU35603.1}.
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DR EMBL; QNQT01000009; RDU35603.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3D8GME3; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000257144; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000257144}.
FT DOMAIN 127..406
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 407..674
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 148..155
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 753 AA; 84485 MW; F8DB9CAF49010012 CRC64;
MKSANFQNGT VHLQTLSRER YQKIYLAGRN GQLSCPACFE PVRLYLGIEH EPHFFHLNDP
GKECPGPTDG NKDTRVIGGF RVPNAKPVGE SFQAAQALAI GGGAATAMLP APEDPHDYLK
LLEAEGFKLD GNQAAAVTET EGPILVLAGA GSGKTRVLTA RTAYMLDVRG ISPSDIMLVT
FTTKAAQEMK SRLLRYPNMK SELLSKLVSG TFHSIFYRIL MHADRARWNG DKLLKKEWQR
ERIIKDAARE LGLSEKDFPF DLALQQIGFW KNSLMSPSEA RPDTEWEETA LLLYERYEAY
KQRQGLFDFD DMLTGCHELF RSSPGILEMY QDRFRYFLID EFQDINKVQY ELIKMLSAKT
KNVCAVGDDD QTIYSFRGSS PEFLLNFEHD FPGARTVLLS QNYRSSNEIV SAANRIISFN
KKRRRKEMAA RSSAGIPPVV FFPYDEEEEA TMVVSDLQEK IAAGADPGDF AILYRTHAGS
RAVFERLASS NLPFRIEQDA ESFYERRTVK TVLGFLSLAV DEDDIQAVAA ILPALFLKQE
VLSDIKAATI LDDCTALEAL GKVKTAHAFQ QKKLQSAVSA LKTMRSLAPY AAIEKIEKEL
GFLDYLKKRG SEGNKLEKGS DDVKDLKVAA KSFDTIPALL EHASHMAAMN REIKNLGKHF
PSAITLSTIH RAKGLEYKTV YILGAVDGGL PHDYALESLR NGDPAPIEEE RRLFYVAITR
AMERLYISVP SMRRNRAANP TRFLTPVRKD LKR
//
