ID A0A3D8GPR9_9BACI Unreviewed; 719 AA.
AC A0A3D8GPR9;
DT 16-JAN-2019, integrated into UniProtKB/TrEMBL.
DT 16-JAN-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN Name=pulA {ECO:0000313|EMBL:RDU36159.1};
GN ORFNames=DRW41_14090 {ECO:0000313|EMBL:RDU36159.1};
OS Neobacillus piezotolerans.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Neobacillus.
OX NCBI_TaxID=2259171 {ECO:0000313|EMBL:RDU36159.1, ECO:0000313|Proteomes:UP000257144};
RN [1] {ECO:0000313|EMBL:RDU36159.1, ECO:0000313|Proteomes:UP000257144}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YLB-04 {ECO:0000313|EMBL:RDU36159.1,
RC ECO:0000313|Proteomes:UP000257144};
RA Yu L., Tang X.;
RT "Bacillus sp. YLB-04 draft genome sequence.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDU36159.1}.
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DR EMBL; QNQT01000006; RDU36159.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3D8GPR9; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000257144; Unassembled WGS sequence.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 2.60.40.2320; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR040697; PulA_N1.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF17999; PulA_N1; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Glycosidase {ECO:0000313|EMBL:RDU36159.1};
KW Hydrolase {ECO:0000313|EMBL:RDU36159.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000257144}.
FT DOMAIN 229..616
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 719 AA; 80688 MW; 6EE16BE1880F8258 CRC64;
MASDNRPFFA YLDAMDTITV LLPLPLKEPE PASFTLESRF GGGEVSIEER HEIEGYLKYI
CRFKDPYAFG TEHWVVSGNG SRTDVQIGAV TRSKEFDREF FYSGDDLGSS CQNGRTSFKV
WAPTATNVKI KIYMPGEGRF ELVKMARGEK GVWSLELGRD LEFARYTYMA FINQEWKEAA
DPYAAAVTVN GTHGVVARLE NTAVPKIPMP PFSHPADAII YETHIRDFTI HPVGGAVHKG
LYKGAGETGT TGPDGKPTGL SHVKELGATH IEFLPFNDFA GVDETNIRAA YNWGYNPVHF
NVPEGSYSTN PDDPYTRIRE LKEMIEAIHS VGIRVIMDVV YNHVFIREKS SFEQLVPGYY
FRYNEHGLPS DGTGVGNDFA SERLMARKFI IDSIIHWIKE YNLDGFRFDL MGILDVQTMA
EIRNACDRLK PGMLIIGEGW ELNTPLHPGE KATIRNQRRL PTIGQFNDRF RDAIKGSTFN
LYERGYALGN GTYTEQAKEA ISGSVGIFSG QPLFSEPGQS VNYVESHDNH TLWDKLEACL
GDSGELLKIK CHRLATSLVL LSQGLPFLHG GQEFFRTKGG DGNSYKSPEN VNWLDWERKA
AFQDNVRYIT GMIEIRKSLS CFRMRENEQI RNTLADLGLP HPLIGFHYKD NWGEGEAILL
VNPTAFIHQL PLPKGEWEVL ADDLHAGVTP LRSFPHHADV AIEPVSIMVL LKKCNEAGN
//
