ID A0A3D8S878_9HELO Unreviewed; 2469 AA.
AC A0A3D8S878;
DT 16-JAN-2019, integrated into UniProtKB/TrEMBL.
DT 16-JAN-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=alpha-1,3-glucan synthase {ECO:0000256|ARBA:ARBA00012688};
DE EC=2.4.1.183 {ECO:0000256|ARBA:ARBA00012688};
GN ORFNames=BP6252_03659 {ECO:0000313|EMBL:RDW82547.1};
OS Coleophoma cylindrospora.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Dermateaceae; Coleophoma.
OX NCBI_TaxID=1849047 {ECO:0000313|EMBL:RDW82547.1, ECO:0000313|Proteomes:UP000256645};
RN [1] {ECO:0000313|EMBL:RDW82547.1, ECO:0000313|Proteomes:UP000256645}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BP6252 {ECO:0000313|EMBL:RDW82547.1,
RC ECO:0000313|Proteomes:UP000256645};
RX PubMed=30018880; DOI=10.5598/imafungus.2018.09.01.13;
RA Wingfield B.D., Bills G.F., Dong Y., Huang W., Nel W.J.,
RA Swalarsk-Parry B.S., Vaghefi N., Wilken P.M., An Z., de Beer Z.W.,
RA De Vos L., Chen L., Duong T.A., Gao Y., Hammerbacher A., Kikkert J.R.,
RA Li Y., Li H., Li K., Li Q., Liu X., Ma X., Naidoo K., Pethybridge S.J.,
RA Sun J., Steenkamp E.T., van der Nest M.A., van Wyk S., Wingfield M.J.,
RA Xiong C., Yue Q., Zhang X.;
RT "IMA Genome-F 9: Draft genome sequence of Annulohypoxylon stygium,
RT Aspergillus mulundensis, Berkeleyomyces basicola (syn. Thielaviopsis
RT basicola), Ceratocystis smalleyi, two Cercospora beticola strains,
RT Coleophoma cylindrospora, Fusarium fracticaudum, Phialophora cf. hyalina,
RT and Morchella septimelata.";
RL IMA Fungus 9:199-223(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->3)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->3)-
CC alpha-D-glucosyl](n+1) + H(+) + UDP; Xref=Rhea:RHEA:19749, Rhea:RHEA-
CC COMP:11150, Rhea:RHEA-COMP:11151, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28100, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC EC=2.4.1.183; Evidence={ECO:0000256|ARBA:ARBA00000687};
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC {ECO:0000256|ARBA:ARBA00006122}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RDW82547.1}.
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DR EMBL; PDLM01000003; RDW82547.1; -; Genomic_DNA.
DR STRING; 1849047.A0A3D8S878; -.
DR Proteomes; UP000256645; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0047657; F:alpha-1,3-glucan synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11323; AmyAc_AGS; 1.
DR CDD; cd03791; GT5_Glycogen_synthase_DULL1-like; 1.
DR CDD; cd06174; MFS; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR PANTHER; PTHR47182; CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATED; 1.
DR PANTHER; PTHR47182:SF2; CELL WALL ALPHA-1,3-GLUCAN SYNTHASE MOK13; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000313|EMBL:RDW82547.1};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000256645};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..2469
FT /note="alpha-1,3-glucan synthase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5017761209"
FT TRANSMEM 1093..1114
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2045..2067
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2079..2096
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2103..2126
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2138..2158
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2170..2191
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2211..2235
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2258..2275
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2295..2316
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2323..2343
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2367..2387
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2394..2417
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2441..2461
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 69..526
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 1660..1679
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1703..1724
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1738..1766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1794..1893
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1703..1719
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1820..1847
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2469 AA; 275281 MW; 0B5F84D31692BC08 CRC64;
MFGFRGAALA WLSLWSSTSR VSALVYDEQY VGYNLNTNKT ATSPHDYWGE WEGHTYMPSP
TNWRFPFYTL FLDRFVNGDP TNDNANGTAF EQDVMQTQLR HGGDLQGLID SLDYIQGMGI
KAIYIAGSIM INQPWGSDAY SPLDLTLLDA HFGDISKWRE MTQAVHDRGM YVVLDNTMGT
MGDLIAFDGY LNSSTPFQLT EHKVQWRDPN RQYLDFAFGE EYNTTCDYPR FWLETGYPVG
TSVTDLMNGC YNSEFDQYGD TEAFGVFPDW QRQLSKFASV QDRLREWLPS VRTKIDLFTC
MQIAQLDIDG IRLDKATQIT IDALGDFAHA TRECARALGK ENFFIPGEIT GGNTFGAIYI
GRGRQPDMQP STISSAVQMT TNSSLEYFIR DADYNALDAG AFHYSIYRSL TRFLGMDGNL
ASGYDTPVNW VDAWNEMLLT NDFINPNTGV FDPRHMYGTA NQDVFRWPAI KNGTEKLLLG
LFITTLHMPG IPLLLWGEEQ AFYVLDNTAS NYIFGRQAMS AATAWQTHGC YLLGSSSYYQ
FPVESATTGC TDDWNMRDHR DPAAPIRNII KHMYQMRENY PSLQDGWFLQ QLSNMTGWIQ
YPGSGTTKTE TGLWSTMRSG FENIQDLSGT GAGNQSVWLV YQNGNTSKTY SFTCSDNDTA
LIAPFDSGTT VRNLFYPFDT VTLKASSQKL GIANQTGYNG CLDSLTMSAW EFKAYVPADT
WVGPGPMITK FSPGHDVSVQ SAVKPGEQES IPIEFHFSQE MNCTALMANM KITSTTEDGK
VAQLDSNTAT CNVVPTEAVT DYIGSIPTTW KFTANLTNVS NGIHSITLTN VSTNSGNDTT
KSVDKFLFRV GQTDNPVVFP RLANYTLGLL HQNTDGSLYV SHKAAGADRF RYSLNWESTW
SNWTTYTGGN TTLETQPWTG TSKQKWDGEH VIMQYFSNLT GSSSYTQHAD LDASQQARRF
PHLFIEGPFN LYGYDAGLKN EMTKNKITGL WEAYFMTEWP GVFQINVWGM NPDGEPDKTV
ILGDVDLDGV LDRMPPSSLS TTLVNYTYRA DTGKHLIGDF IYPPAPYLGY KVVLNDGDYA
YGLVPYGSRA QQMILAFLMI IIPVFTGGLS IWLFMKSFYA VKFNAVGISE PHHFIPLAIR
RKLKRQEKGE KDMALGVPSP IMMHDNPSNV GLSADAGGGR RRTVLIATME YDIEDWEIKI
KIGGLGVMAQ LMGKNLGHQD LIWVVPCVGG IDYPVDTPAA SMDVTILGKP YEIQVQYHTL
RNITYVLLDA PVFRAQSKSE PYPARMDDLD SAVYYSAWNQ CIALAIQRFP IDLYHINDYH
GSLAPVHLLP RTIPCCLSLH NAEFQGLWPM RTKKERAEVC AVYNLSEEVV TKYVQFGDVF
NLLHAGASYL RVHQKGFGAV GVSKKYGKRS WARYPIFWGL NKIGNLPNPD PSDTGEWTGE
QDKEEAVVNQ DFEANRGELK RQAQEWAGLD QNPKADLLVF VGRWSMQKGI DLIADVMPAV
LEENPNVQLI CVGPVIDLYG KFAALKLDVM MKKYKGRVFS KPEFTALPPF IFSGAEFALI
PSRDEPFGLV AVEFGRKGAL GIGARVGGLG QMPGWWFTVE STTTTHLLHQ FKMAIKEALS
SKLETRQMMR ARSAKQRFPV AQWVHDLETL QGKAIKIHLK EEGKSSGRPS SRGNDSRSLF
ASNRRVSRHM LGMNASESQI DVSTISNSTR PSSPSGTIEP PAANAGLSRK LSLGYRAGPG
HRVMKRGRAN DSSASLGMGL ANEPITDVDE DTDFEDRTMS IYDEYVLSPE ELEAERQREQ
QHFHAQSPGL PGHGGLLSPH GNSFSGSPRT SFANSPRVTQ RMLSGQPESD YSLPIAARGS
AIYSGPPSPS GADSPGADDV LLPPAPFGSD QASHRASMLS LSSVVGDKTD FKLQKVDPFF
TDTQGEYYEA FARSLDNLNG KNSEQLLCIE DYLVKSEKKW FDKFRDAKLG RGSFVPPTPS
ALGAGSRRGS VSDSFMNETI VHSRQNSEND GHGGSQEEND QFLLGKDYKP PTGLRLIMQY
RYSDWPVYSF FLAFGQIIAA NSYQITLLSG TVGQTAEKLY ITAAIYLVSS IFWWMVFIRL
KSIYVLSCPF FFYGMAFFLL GMAPFVSGTA SRGWIQNVAT GFYSVAASSG SIFFALNFGD
EGGSPIKSWV YRACLIQGTQ QAYNVVLWWW GDYLNKRSAD GLSATFTTAS TTMSMITIPV
ACLIWACGVI LFLGLPDYYR QKPGKVPSFY TSLFRRKIVM WFFVTVMIQN FFLSTNYGRN
WMYLFSSNNA TAWEIILLII LFFGGVWALF LWFFGMLSKD HSWILPIFAI GLGAPRWCQQ
LWACTPIGQY VPWAGSPLGS ALFSRSLWLW LGVLDALQGA GFGMILLQTL TRLHIAFTLI
AAQVIGSIFT IIARAWANNN TGPGDVFPDF SLGAYPGITK AWFWIGLLFQ LAICGGFFTF
FRKEQLSKP
//
