ID   A0A3D8YU98_9BACL        Unreviewed;       357 AA.
AC   A0A3D8YU98;
DT   16-JAN-2019, integrated into UniProtKB/TrEMBL.
DT   16-JAN-2019, sequence version 1.
DT   24-JAN-2024, entry version 14.
DE   SubName: Full=M42 family peptidase {ECO:0000313|EMBL:REB06518.1};
GN   ORFNames=DVB69_12515 {ECO:0000313|EMBL:REB06518.1};
OS   Sporosarcina sp. BI001-red.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae; Sporosarcina.
OX   NCBI_TaxID=2282866 {ECO:0000313|EMBL:REB06518.1, ECO:0000313|Proteomes:UP000256461};
RN   [1] {ECO:0000313|EMBL:REB06518.1, ECO:0000313|Proteomes:UP000256461}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BI001-red {ECO:0000313|EMBL:REB06518.1,
RC   ECO:0000313|Proteomes:UP000256461};
RA   Rajandas H., Sivachandran P., Mai C.W., Cheong K.W., Teh C.S.J.,
RA   Lim E.S.H., Yap I.K.S., Convey P., Chong C.W.;
RT   "Draft Genome of Sporosarcina subantarcticus.";
RL   Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000256|PIRSR:PIRSR001123-2};
CC   -!- SIMILARITY: Belongs to the peptidase M42 family.
CC       {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:REB06518.1}.
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DR   EMBL; QQAK01000013; REB06518.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3D8YU98; -.
DR   OrthoDB; 9772053at2; -.
DR   Proteomes; UP000256461; Unassembled WGS sequence.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd05656; M42_Frv; 1.
DR   Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR008007; Peptidase_M42.
DR   InterPro; IPR023367; Peptidase_M42_dom2.
DR   PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR32481:SF0; AMINOPEPTIDASE YPDE-RELATED; 1.
DR   Pfam; PF05343; Peptidase_M42; 1.
DR   PIRSF; PIRSF001123; PepA_GA; 1.
DR   SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000256461}.
FT   ACT_SITE        212
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT   BINDING         66
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         180
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         180
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         213
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         235
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         320
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ   SEQUENCE   357 AA;  39104 MW;  0145BBDA04B0995A CRC64;
     MNNETLAMFK TLTELPGAPG NEHAVRKYLR GELEKYSDEM IQDNLGGIFG VRKGPENSPK
     IMVAGHMDEV GFMISGITEN GMLRFQTLGG WWNQVMLAQR VEIITDKGVI PGVIGSIPPH
     LLTDEQRAKP MDVKNMLIDI GADDLEDAKR IGIRPGQQAL PVCPFTPMAN PKKIMAKAWD
     NRYGCGLALE LLKEVKDVEL PNQLFSGATV MEEVGLRGAQ TAANMIQPDL FFGLDASPAN
     DMSGSKTEFG QLGKGTLLRI LDRTMITHRG IREFILDTAE TNNIPYQYFV SQGGTDAGKV
     HISNEGVPSA IIGICSRYIH TSSSIIHTDD YAAAKELLVK LVTTCDKTTL ETIKQNV
//