ID A0A3F2XWQ3_DEKBR Unreviewed; 170 AA.
AC A0A3F2XWQ3;
DT 16-JAN-2019, integrated into UniProtKB/TrEMBL.
DT 16-JAN-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=6,7-dimethyl-8-ribityllumazine synthase {ECO:0000256|ARBA:ARBA00012664, ECO:0000256|RuleBase:RU003795};
DE Short=DMRL synthase {ECO:0000256|RuleBase:RU003795};
DE EC=2.5.1.78 {ECO:0000256|ARBA:ARBA00012664, ECO:0000256|RuleBase:RU003795};
GN Name=RIB4 {ECO:0000313|EMBL:VUG16509.1};
GN ORFNames=DEBR0S1_18492G {ECO:0000313|EMBL:VUG16509.1}, HII12_004147
GN {ECO:0000313|EMBL:KAF6008398.1};
OS Dekkera bruxellensis (Brettanomyces custersii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Pichiaceae; Brettanomyces.
OX NCBI_TaxID=5007 {ECO:0000313|EMBL:VUG16509.1, ECO:0000313|Proteomes:UP000478008};
RN [1] {ECO:0000313|EMBL:VUG16509.1, ECO:0000313|Proteomes:UP000478008}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Friedrich A., Schacherer J.;
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAF6008398.1, ECO:0000313|Proteomes:UP000568158}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AWRI1613 {ECO:0000313|EMBL:KAF6008398.1,
RC ECO:0000313|Proteomes:UP000568158};
RX PubMed=32592028;
RA Varela C., Bartel C., Onetto C., Borneman A.;
RT "Targeted gene deletion in Brettanomyces bruxellensis with an expression-
RT free CRISPR-Cas9 system.";
RL Appl. Microbiol. Biotechnol. 104:7105-7115(2020).
CC -!- FUNCTION: Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by
CC condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-
CC butanone 4-phosphate. This is the penultimate step in the biosynthesis
CC of riboflavin. {ECO:0000256|RuleBase:RU003795}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-
CC ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2
CC H2O + phosphate; Xref=Rhea:RHEA:26152, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15934, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:58201, ChEBI:CHEBI:58830; EC=2.5.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001697,
CC ECO:0000256|RuleBase:RU003795};
CC -!- PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; riboflavin
CC from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-
CC ribitylamino)uracil: step 1/2. {ECO:0000256|ARBA:ARBA00004917,
CC ECO:0000256|RuleBase:RU003795}.
CC -!- SIMILARITY: Belongs to the DMRL synthase family.
CC {ECO:0000256|ARBA:ARBA00007424, ECO:0000256|RuleBase:RU003795}.
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DR EMBL; JABCYN010000036; KAF6008398.1; -; Genomic_DNA.
DR EMBL; CABFWN010000001; VUG16509.1; -; Genomic_DNA.
DR STRING; 5007.A0A3F2XWQ3; -.
DR UniPathway; UPA00275; UER00404.
DR Proteomes; UP000478008; Unassembled WGS sequence.
DR Proteomes; UP000568158; Unassembled WGS sequence.
DR GO; GO:0009349; C:riboflavin synthase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0000906; F:6,7-dimethyl-8-ribityllumazine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009231; P:riboflavin biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd09209; Lumazine_synthase-I; 1.
DR Gene3D; 3.40.50.960; Lumazine/riboflavin synthase; 1.
DR HAMAP; MF_00178; Lumazine_synth; 1.
DR InterPro; IPR034964; LS.
DR InterPro; IPR002180; LS/RS.
DR InterPro; IPR036467; LS/RS_sf.
DR NCBIfam; TIGR00114; lumazine-synth; 1.
DR PANTHER; PTHR21058:SF0; 6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE; 1.
DR PANTHER; PTHR21058; 6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE; 1.
DR Pfam; PF00885; DMRL_synthase; 1.
DR SUPFAM; SSF52121; Lumazine synthase; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000478008};
KW Riboflavin biosynthesis {ECO:0000256|ARBA:ARBA00022619,
KW ECO:0000256|RuleBase:RU003795};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003795}.
SQ SEQUENCE 170 AA; 18245 MW; B50C02E3C5DD2AF4 CRC64;
MAISGRAKED QTYDGSGVKV AIIHARWNEE VIDSLVEGCI RRLKELGVHS SNITVESVPG
SYELPVGVAS LAQEKDVDAI VAIGCLVKGD TMHFEYISEA VSNQLMSIQF KIGKPVIFGL
LTCLTYDQAK ARAGLIPGQA HNHGVDWAEC AVEMSVKFGS GLNKLARSGN
//
