ID A0A3G3JWP1_9BACL Unreviewed; 812 AA.
AC A0A3G3JWP1;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=NAD(P)/FAD-dependent oxidoreductase {ECO:0000313|EMBL:AYQ72277.1};
GN ORFNames=EAV92_06660 {ECO:0000313|EMBL:AYQ72277.1};
OS Cohnella candidum.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Cohnella.
OX NCBI_TaxID=2674991 {ECO:0000313|EMBL:AYQ72277.1, ECO:0000313|Proteomes:UP000269097};
RN [1] {ECO:0000313|EMBL:AYQ72277.1, ECO:0000313|Proteomes:UP000269097}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=18JY8-7 {ECO:0000313|EMBL:AYQ72277.1,
RC ECO:0000313|Proteomes:UP000269097};
RA Srinivasan S., Kim M.K.;
RT "Genome Sequence of Cohnella sp.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRNR:PIRNR037149};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- COFACTOR:
CC Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC Evidence={ECO:0000256|ARBA:ARBA00001929};
CC -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC {ECO:0000256|ARBA:ARBA00005096}.
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000256|ARBA:ARBA00010429}.
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DR EMBL; CP033433; AYQ72277.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3G3JWP1; -.
DR KEGG; coh:EAV92_06660; -.
DR UniPathway; UPA00653; -.
DR Proteomes; UP000269097; Chromosome.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniRule.
DR CDD; cd19943; NirB_Fer2_BFD-like_1; 1.
DR CDD; cd19944; NirB_Fer2_BFD-like_2; 1.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR Gene3D; 3.30.413.10; Sulfite Reductase Hemoprotein, domain 1; 1.
DR Gene3D; 3.90.480.10; Sulfite Reductase Hemoprotein;Domain 2; 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR InterPro; IPR036136; Nit/Sulf_reduc_fer-like_dom_sf.
DR InterPro; IPR012744; Nitri_red_NirB.
DR InterPro; IPR017121; Nitrite_Rdtase_lsu.
DR InterPro; IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR InterPro; IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
DR InterPro; IPR041575; Rubredoxin_C.
DR NCBIfam; TIGR02374; nitri_red_nirB; 1.
DR PANTHER; PTHR43809; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR PANTHER; PTHR43809:SF1; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR Pfam; PF04324; Fer2_BFD; 2.
DR Pfam; PF01077; NIR_SIR; 1.
DR Pfam; PF03460; NIR_SIR_ferr; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF18267; Rubredoxin_C; 1.
DR PIRSF; PIRSF037149; NirB; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR PRINTS; PR00397; SIROHAEM.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
DR SUPFAM; SSF56014; Nitrite and sulphite reductase 4Fe-4S domain-like; 1.
DR SUPFAM; SSF55124; Nitrite/Sulfite reductase N-terminal domain-like; 1.
DR PROSITE; PS00365; NIR_SIR; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRNR:PIRNR037149};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|PIRNR:PIRNR037149}; Heme {ECO:0000256|ARBA:ARBA00022617};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063,
KW ECO:0000256|PIRNR:PIRNR037149};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000269097}.
FT DOMAIN 6..282
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
FT DOMAIN 318..385
FT /note="NADH-rubredoxin oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF18267"
FT DOMAIN 421..470
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 485..534
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 560..622
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
FT DOMAIN 630..767
FT /note="Nitrite/sulphite reductase 4Fe-4S"
FT /evidence="ECO:0000259|Pfam:PF01077"
SQ SEQUENCE 812 AA; 88408 MW; B2508D87AA2D6052 CRC64;
MDNAKKLVMI GNGMAGVRTI EHLLKLAPGA YDITIIGAEP HPNYNRILLS SVLAGGADMK
DIVINDWNWY EENRIRLLSG HTAIKIDTKS NTVTTSRGTV AEYDALILAT GSNPFMLPLP
GADKEGVIAF RDIRDCEIMM ETSRKYKKAA VIGGGLLGLE AARGLLHLGM DVSVVHIHPY
LMERQLDQAA SKMLQKELEA QGMKFLLQKN SESIYGRGRV KGLRFTDGSE LEADLIVMAV
GIRPNVELAK SSGIDVNRGV VVNDFMETNV PGVYAVGECA EHRGIAYGLV APLYEQGAVL
AKRLAGVETA GYAGSVTSTK LKVSGVDVFS AGRYDEPSGT RAMRYQDEVE GVYKKIVLQD
DKLVGAVLFG DTGDGAQLFS LIKSGESVKG KEKELLLGFA PGGSSAASPE SRLEAMAGDE
IVCGCNGVSK ATIQDAILNK DCTSVSAIKA CTKASASCGG CKPLVEGLLK VYGGDAAGAP
VKEGICGCTT LSRDEIVAEI KRMELKSVKE VMHVLGWDNP EGCSKCRPSL NYYLGMLWPM
EYKDEKESRF TNERYHANIQ KDGTYSVVPR IYGGVTSPAE LKKIAEVAEK FDVPMVKFTG
GQRLDLLGVK KEDLPKMWEE LDMPSGHAYG KALRTVKTCV GETFCRFGTQ DSIGMGIRLE
KEFERLNTPA KVKLAVSGCP RNCAEATIKD FGVVAIDGGW ELHVGGNGGV HVRATDLLCV
VKTQDEVVEW ASAFLQHYRE NAQWNERTSV WIERVGLDSV KKALENKEDR EALYARIKTA
LSQTVDPWKE IVSERELRKN FESLPEPAQV QI
//
